Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PG878_RS17985 Genome accession   NZ_CP115873
Coordinates   4237774..4238913 (+) Length   379 a.a.
NCBI ID   WP_209032473.1    Uniprot ID   -
Organism   Xanthomonas hawaiiensis strain A6251     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4232774..4243913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG878_RS17960 (PG878_17945) - 4232986..4234185 (-) 1200 WP_209032471.1 cystathionine gamma-synthase -
  PG878_RS17965 (PG878_17950) - 4234268..4235638 (-) 1371 WP_209032472.1 pyridoxal-phosphate dependent enzyme -
  PG878_RS17970 (PG878_17955) - 4235831..4236049 (-) 219 WP_010340228.1 YdcH family protein -
  PG878_RS17975 (PG878_17960) - 4236392..4237264 (-) 873 WP_209032492.1 hypothetical protein -
  PG878_RS17980 (PG878_17965) - 4237282..4237674 (-) 393 WP_010340230.1 DUF4398 domain-containing protein -
  PG878_RS17985 (PG878_17970) pilU 4237774..4238913 (+) 1140 WP_209032473.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PG878_RS17990 (PG878_17975) maiA 4239537..4240193 (-) 657 WP_209032474.1 maleylacetoacetate isomerase -
  PG878_RS17995 (PG878_17980) - 4240283..4241272 (-) 990 WP_209032475.1 fumarylacetoacetate hydrolase family protein -
  PG878_RS18000 (PG878_17985) - 4241618..4242988 (+) 1371 WP_209032476.1 DEAD/DEAH box helicase -
  PG878_RS18005 (PG878_17990) queD 4243167..4243526 (+) 360 WP_017916250.1 6-carboxytetrahydropterin synthase QueD -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42092.37 Da        Isoelectric Point: 6.2387

>NTDB_id=774121 PG878_RS17985 WP_209032473.1 4237774..4238913(+) (pilU) [Xanthomonas hawaiiensis strain A6251]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGATGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALQDAGR
FRVNVFKQRGEVGMVIRAIRSKIPSIEELHLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDYRNSTTTGHILTIED
PIEYLHKHKLSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLSLNLRAVISQRLVKDNSGRRRPASEVLLNTPMIRDLLRRGQVHEIKQAMEESLEEGMETFDQCL
FRMVKQGQIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYDSGPSAAPRITHGFV

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=774121 PG878_RS17985 WP_209032473.1 4237774..4238913(+) (pilU) [Xanthomonas hawaiiensis strain A6251]
ATGGATATCGGCTATTTCCTGAAGCTGATGACCGAAAAGAACGCCTCGGACATGTTCCTGACCACAGGCGCACCGGTCTA
TATCAAGATCGAAGGCAAGCTGTACCCGCTCGGCGCCACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGGGAGCTGGAATTGAACATGGCCATCGCCCTGCAGGACGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCAAGATCCCCAGCATCGA
GGAGCTGCACCTGCCGCAGGTGCTCAAGGACGTGATCATGACCCCGCGCGGGCTGGTCCTGGTGGTCGGCTCGACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACTACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAT
CCGATCGAATACCTGCACAAGCACAAGCTGTCCATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCCATGCGCGAGGCACCGGACGTGATCCTGATCGGCGAGATCCTGGACGCGGAGACCATGGAGGCGG
CCATCGCCTTCGCCGAGACAGGACACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGTCGCTGAACCTGCGCGCGGTGATCTCGCAGCG
CCTGGTCAAGGACAACAGCGGCCGCCGCCGGCCGGCCTCGGAGGTGCTGCTTAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTCCACGAGATCAAGCAGGCGATGGAGGAGTCGCTGGAGGAAGGCATGGAGACCTTCGACCAGTGCCTG
TTCCGCATGGTCAAGCAGGGCCAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGATTCGCGCGACGGCCTGGCGCTGAA
ATTCCGCCTGTCCGAAGGCGGCAGCGGCGAGCACGACCCCTACGCCGACTACGACAGCGGCCCCAGCGCCGCGCCGCGCA
TCACCCACGGCTTCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(47-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

94.723

0.493
  pilU Vibrio cholerae strain A1552

49.722

94.987

0.472
  pilU Acinetobacter baylyi ADP1

50

93.404

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.18

99.736

0.441
  pilT Pseudomonas aeruginosa PAK

43.491

89.182

0.388
  pilT Acinetobacter baumannii D1279779

42.604

89.182

0.38
  pilT Acinetobacter baumannii strain A118

42.604

89.182

0.38
  pilT Acinetobacter nosocomialis M2

42.604

89.182

0.38
  pilT Acinetobacter baylyi ADP1

41.471

89.71

0.372
  pilT Legionella pneumophila strain ERS1305867

41.716

89.182

0.372
  pilT Legionella pneumophila strain Lp02

41.716

89.182

0.372
  pilT Neisseria meningitidis 8013

40.346

91.557

0.369
  pilT Neisseria gonorrhoeae MS11

40.346

91.557

0.369
  pilT Pseudomonas stutzeri DSM 10701

41.916

88.127

0.369
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364