Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PF325_RS21275 Genome accession   NZ_CP115689
Coordinates   4358222..4359655 (+) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain 2020CK-00204     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4353222..4364655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF325_RS21245 (PF325_21245) - 4353789..4354766 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  PF325_RS21250 (PF325_21250) kdsD 4354780..4355766 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  PF325_RS21255 (PF325_21255) kdsC 4355781..4356347 (+) 567 WP_017693909.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PF325_RS21260 (PF325_21260) lptC 4356344..4356919 (+) 576 WP_032103286.1 LPS export ABC transporter periplasmic protein LptC -
  PF325_RS21265 (PF325_21265) lptA 4356888..4357442 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PF325_RS21270 (PF325_21270) lptB 4357449..4358174 (+) 726 WP_023304493.1 LPS export ABC transporter ATP-binding protein -
  PF325_RS21275 (PF325_21275) rpoN/rpoN1 4358222..4359655 (+) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  PF325_RS21280 (PF325_21280) hpf 4359678..4359965 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  PF325_RS21285 (PF325_21285) ptsN 4360048..4360539 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PF325_RS21290 (PF325_21290) rapZ 4360585..4361439 (+) 855 WP_003861870.1 RNase adapter RapZ -
  PF325_RS21295 (PF325_21295) npr 4361436..4361708 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  PF325_RS21300 (PF325_21300) mtgA 4361748..4362473 (-) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PF325_RS21305 (PF325_21305) elbB 4362470..4363123 (-) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=773483 PF325_RS21275 WP_003861864.1 4358222..4359655(+) (rpoN/rpoN1) [Enterobacter hormaechei strain 2020CK-00204]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=773483 PF325_RS21275 WP_003861864.1 4358222..4359655(+) (rpoN/rpoN1) [Enterobacter hormaechei strain 2020CK-00204]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACTCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATCCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48