Detailed information NTG0772978

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PF552_RS14615
• Genome accession: NZ_CP115632
• Coordinates: 3019244..3020362 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 2022CK-00063
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3014244..3025362

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF552_RS14605 (PF552_14605)	-	3017360..3018052 (-)	693	WP_031959811.1	YggS family pyridoxal phosphate-dependent enzyme	-
PF552_RS14610 (PF552_14610)	pilT	3018179..3019216 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
PF552_RS14615 (PF552_14615)	pilU	3019244..3020362 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PF552_RS14620 (PF552_14620)	fur	3020432..3020869 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
PF552_RS14625 (PF552_14625)	bamE	3020981..3021379 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
PF552_RS14630 (PF552_14630)	-	3021434..3021757 (-)	324	WP_000442585.1	RnfH family protein	-
PF552_RS14635 (PF552_14635)	-	3021757..3022824 (-)	1068	WP_031959810.1	membrane protein	-
PF552_RS14640 (PF552_14640)	-	3022977..3023432 (-)	456	WP_000782976.1	bacteriohemerythrin	-
PF552_RS14645 (PF552_14645)	-	3023685..3024533 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=772978 PF552_RS14615 WP_000347036.1 3019244..3020362(+) (pilU) [Acinetobacter baumannii strain 2022CK-00063]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=772978 PF552_RS14615 WP_000347036.1 3019244..3020362(+) (pilU) [Acinetobacter baumannii strain 2022CK-00063]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392