Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   PF449_RS17240 Genome accession   NZ_CP115623
Coordinates   3582518..3582940 (-) Length   140 a.a.
NCBI ID   WP_031961381.1    Uniprot ID   A0A836M1M3
Organism   Acinetobacter baumannii strain 2022CK-00340     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3577518..3587940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF449_RS17210 (PF449_17210) - 3578046..3579080 (+) 1035 WP_001189451.1 lipase secretion chaperone -
  PF449_RS17215 (PF449_17215) - 3579195..3580136 (+) 942 WP_104131029.1 triacylglycerol lipase -
  PF449_RS17220 (PF449_17220) rplS 3580190..3580558 (-) 369 WP_000014562.1 50S ribosomal protein L19 -
  PF449_RS17225 (PF449_17225) trmD 3580766..3581506 (-) 741 WP_031961379.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  PF449_RS17230 (PF449_17230) rimM 3581552..3582100 (-) 549 WP_031961380.1 ribosome maturation factor RimM -
  PF449_RS17235 (PF449_17235) rpsP 3582120..3582371 (-) 252 WP_000260334.1 30S ribosomal protein S16 -
  PF449_RS17240 (PF449_17240) pilE 3582518..3582940 (-) 423 WP_031961381.1 type IV pilin protein Machinery gene
  PF449_RS17245 (PF449_17245) - 3582940..3583503 (-) 564 WP_031961383.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PF449_RS17250 (PF449_17250) - 3583513..3587583 (-) 4071 WP_031961384.1 PilC/PilY family type IV pilus protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15404.52 Da        Isoelectric Point: 7.7994

>NTDB_id=772858 PF449_RS17240 WP_031961381.1 3582518..3582940(-) (pilE) [Acinetobacter baumannii strain 2022CK-00340]
MKKYEGFTLIELMIVVAIIAILAAIAYPSYTQYKIRTNRADVQSEMMQTAQRLQSYYVINHNYTSATLNNGLTTKDYPAS
NPIYTIALVTNSQTWTLTATPISTSSQAGDGIICLNDQGQKFWAKGATVCALSASSSWTE

Nucleotide


Download         Length: 423 bp        

>NTDB_id=772858 PF449_RS17240 WP_031961381.1 3582518..3582940(-) (pilE) [Acinetobacter baumannii strain 2022CK-00340]
ATGAAAAAATATGAAGGTTTTACCCTCATAGAGCTAATGATTGTGGTTGCTATTATTGCAATTTTGGCAGCAATTGCTTA
CCCCTCCTATACACAATATAAAATTCGGACGAATCGTGCAGATGTGCAAAGTGAAATGATGCAAACCGCACAACGTCTAC
AAAGTTACTATGTGATTAATCATAATTATACTAGTGCTACACTCAATAATGGTTTAACTACTAAAGATTATCCAGCATCC
AATCCAATTTATACTATTGCACTAGTCACAAACTCTCAAACATGGACGCTTACTGCAACTCCTATTTCTACTAGCTCACA
AGCAGGTGATGGAATTATTTGTTTAAATGACCAAGGGCAAAAATTTTGGGCAAAAGGTGCCACAGTTTGTGCATTATCTG
CTTCATCGAGTTGGACCGAGTAA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(28-114)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A836M1M3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Acinetobacter baumannii D1279779

64.493

98.571

0.636
  comF Acinetobacter baylyi ADP1

58.156

100

0.586
  pilA Pseudomonas aeruginosa PAK

35.374

100

0.371