Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PF449_RS02595 Genome accession   NZ_CP115623
Coordinates   545558..546358 (+) Length   266 a.a.
NCBI ID   WP_004738086.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain 2022CK-00340     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 540558..551358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF449_RS02570 (PF449_02570) - 541468..542766 (+) 1299 WP_109067365.1 glycosyltransferase family 1 protein -
  PF449_RS02575 (PF449_02575) - 542791..543342 (+) 552 WP_000669565.1 phosphatase PAP2 family protein -
  PF449_RS02580 (PF449_02580) iscX 543442..543642 (+) 201 WP_000524329.1 Fe-S cluster assembly protein IscX -
  PF449_RS02585 (PF449_02585) ndk 543756..544187 (+) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  PF449_RS02590 (PF449_02590) rlmN 544310..545542 (+) 1233 WP_031959661.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  PF449_RS02595 (PF449_02595) pilF 545558..546358 (+) 801 WP_004738086.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  PF449_RS02600 (PF449_02600) - 546349..547140 (+) 792 WP_031959662.1 helix-turn-helix domain-containing protein -
  PF449_RS02605 (PF449_02605) ispG 547162..548277 (+) 1116 WP_000572095.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  PF449_RS02610 (PF449_02610) hisS 548274..549566 (+) 1293 WP_000095254.1 histidine--tRNA ligase -
  PF449_RS02615 (PF449_02615) - 549595..550293 (+) 699 WP_000058788.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30094.44 Da        Isoelectric Point: 9.2307

>NTDB_id=772840 PF449_RS02595 WP_004738086.1 545558..546358(+) (pilF) [Acinetobacter baumannii strain 2022CK-00340]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRSLDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQARNNYGTYLYQMERYNDAIEQFRIAGATLGYDQRYQALENLGRIYLKLGDI
ASAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGLRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=772840 PF449_RS02595 WP_004738086.1 545558..546358(+) (pilF) [Acinetobacter baumannii strain 2022CK-00340]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGTCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ATTCTGCAAAGCGTTCTCTTGACCAAGCGTTAAGTGTCGATAGTCGTGATGCAACGGCAAATATGATGATGGGTATTTTA
TTGCAGCAAGAAGGCAGTAAACCCAATTTAGAAAAAGCAGAACATTACTTTAAGCGTGCAATTTCGTCTGAACCAGACAA
TGCTCAGGCACGTAATAATTATGGTACGTATTTGTACCAAATGGAGCGTTATAATGATGCTATTGAGCAATTTCGTATTG
CTGGTGCAACATTAGGCTATGATCAACGTTACCAGGCCTTAGAAAATCTGGGGCGAATTTATTTAAAACTAGGCGATATT
GCGAGTGCTGAAAAAACATTCAAACAAGCACTTCTTGCTAACCGCGACTCTTACATTTCAATGTTAGAGTTAGCAGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAACAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCACGCGCACTTTGGATTGGTTTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTTTTAGTAAACCAG
TTACGTGCTTTATTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-68)

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

100

100

1
  pilF Acinetobacter baylyi ADP1

74.231

97.744

0.726