Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QMY53_RS12500 Genome accession   NZ_CP127265
Coordinates   2550092..2550769 (-) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli TW14425     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2545092..2555769
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMY53_RS12485 (QMY53_02447) chiA 2546459..2549152 (+) 2694 WP_000773151.1 bifunctional chitinase/lysozyme -
  QMY53_RS12490 (QMY53_02448) bfd 2549321..2549515 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QMY53_RS12495 (QMY53_02449) bfr 2549587..2550063 (+) 477 WP_000675504.1 bacterioferritin -
  QMY53_RS12500 (QMY53_02450) pilD 2550092..2550769 (-) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  QMY53_RS12505 (QMY53_02451) gspM 2550769..2551230 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  QMY53_RS12510 (QMY53_02452) gspL 2551227..2552390 (-) 1164 WP_001115230.1 type II secretion system protein GspL -
  QMY53_RS12515 (QMY53_02453) gspK 2552405..2553388 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  QMY53_RS12520 (QMY53_02454) gspJ 2553381..2553968 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  QMY53_RS12525 (QMY53_02455) gspI 2553961..2554338 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  QMY53_RS12530 (QMY53_02456) gspH 2554335..2554844 (-) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  QMY53_RS12535 (QMY53_02457) gspG 2554852..2555289 (-) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=771828 QMY53_RS12500 WP_000178161.1 2550092..2550769(-) (pilD) [Escherichia coli TW14425]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=771828 QMY53_RS12500 WP_000178161.1 2550092..2550769(-) (pilD) [Escherichia coli TW14425]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378