Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   GVV68_RS25265 Genome accession   NZ_CP115307
Coordinates   4131550..4132593 (-) Length   347 a.a.
NCBI ID   WP_001013178.1    Uniprot ID   -
Organism   Bacillus cereus strain BC-01     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4126550..4137593
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GVV68_RS25220 (GVV68_25225) - 4126981..4127181 (-) 201 WP_000106082.1 YqzE family protein -
  GVV68_RS25225 (GVV68_25230) aroK 4127220..4127717 (-) 498 WP_000836657.1 shikimate kinase AroK -
  GVV68_RS25230 (GVV68_25235) - 4127833..4128483 (-) 651 WP_000183859.1 2OG-Fe(II) oxygenase -
  GVV68_RS25235 (GVV68_25240) comGG 4128660..4129031 (-) 372 WP_001231273.1 competence type IV pilus minor pilin ComGG -
  GVV68_RS25240 (GVV68_25245) comGF 4129028..4129498 (-) 471 WP_000923099.1 competence type IV pilus minor pilin ComGF -
  GVV68_RS25245 (GVV68_25250) - 4129468..4129770 (-) 303 WP_000829473.1 hypothetical protein -
  GVV68_RS25250 (GVV68_25255) comGD 4129763..4130218 (-) 456 WP_000813142.1 competence type IV pilus minor pilin ComGD -
  GVV68_RS25255 (GVV68_25260) comGC 4130215..4130514 (-) 300 WP_001178686.1 competence type IV pilus major pilin ComGC -
  GVV68_RS25260 (GVV68_25265) comGB 4130526..4131557 (-) 1032 WP_000475183.1 competence type IV pilus assembly protein ComGB -
  GVV68_RS25265 (GVV68_25270) comGA 4131550..4132593 (-) 1044 WP_001013178.1 competence type IV pilus ATPase ComGA Machinery gene
  GVV68_RS25270 (GVV68_25275) - 4132799..4133494 (+) 696 WP_000434124.1 helix-turn-helix transcriptional regulator -
  GVV68_RS25275 (GVV68_25280) - 4133621..4133863 (+) 243 WP_000375619.1 DUF2626 domain-containing protein -
  GVV68_RS25280 (GVV68_25285) - 4133971..4135365 (+) 1395 WP_001094333.1 L-cystine transporter -
  GVV68_RS25285 (GVV68_25290) - 4135445..4135837 (-) 393 WP_000875390.1 hypothetical protein -
  GVV68_RS25290 (GVV68_25295) - 4135925..4136140 (-) 216 WP_001008326.1 DUF3912 family protein -
  GVV68_RS25295 (GVV68_25300) - 4136393..4136704 (+) 312 WP_001093237.1 hypothetical protein -
  GVV68_RS25300 (GVV68_25305) - 4136739..4137227 (-) 489 WP_000764261.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39127.50 Da        Isoelectric Point: 8.5482

>NTDB_id=771791 GVV68_RS25265 WP_001013178.1 4131550..4132593(-) (comGA) [Bacillus cereus strain BC-01]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYEAGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGHELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=771791 GVV68_RS25265 WP_001013178.1 4131550..4132593(-) (comGA) [Bacillus cereus strain BC-01]
ATGAATGGGATTGAAATTTTCGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCAGTACAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTTGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTGTTTACTGGACCAACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAAGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAGCAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGGGATGAGGAAACAGCAAAAATAGCTATAAGGGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTACTATATGGACATGAGTTAAAACAAGCACTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.196

100

0.562