Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QQ972_RS02065 Genome accession   NZ_CP127255
Coordinates   401611..402288 (+) Length   225 a.a.
NCBI ID   WP_285984922.1    Uniprot ID   -
Organism   Escherichia coli strain Q5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 396611..407288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQ972_RS02030 (QQ972_02030) gspG 397091..397528 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  QQ972_RS02035 (QQ972_02035) gspH 397536..398045 (+) 510 WP_001076035.1 type II secretion system minor pseudopilin GspH -
  QQ972_RS02040 (QQ972_02040) gspI 398042..398419 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  QQ972_RS02045 (QQ972_02045) gspJ 398412..398999 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  QQ972_RS02050 (QQ972_02050) gspK 398992..399975 (+) 984 WP_001058507.1 type II secretion system minor pseudopilin GspK -
  QQ972_RS02055 (QQ972_02055) gspL 399990..401153 (+) 1164 WP_285984921.1 type II secretion system protein GspL -
  QQ972_RS02060 (QQ972_02060) gspM 401150..401611 (+) 462 WP_001445363.1 type II secretion system protein GspM -
  QQ972_RS02065 (QQ972_02065) pilD 401611..402288 (+) 678 WP_285984922.1 prepilin peptidase GspO Machinery gene
  QQ972_RS02070 (QQ972_02070) bfr 402317..402793 (-) 477 WP_000675504.1 bacterioferritin -
  QQ972_RS02075 (QQ972_02075) bfd 402865..403058 (-) 194 Protein_403 bacterioferritin-associated ferredoxin -
  QQ972_RS02080 (QQ972_02080) chiA 403227..405929 (-) 2703 WP_089638693.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24854.75 Da        Isoelectric Point: 7.6364

>NTDB_id=771633 QQ972_RS02065 WP_285984922.1 401611..402288(+) (pilD) [Escherichia coli strain Q5]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLILPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=771633 QQ972_RS02065 WP_285984922.1 401611..402288(+) (pilD) [Escherichia coli strain Q5]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCATCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.567

100

0.378