Detailed information NTG0771436

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP88_RS06220
• Genome accession: NZ_CP115291
• Coordinates: 1298387..1299535 (+)
• Length: 382 a.a.
• NCBI ID: WP_003110016.1
• Uniprot ID: A0A0H2ZL88
• Organism: Pseudomonas aeruginosa strain F003
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1293387..1304535

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP88_RS06195 (PCP88_06195)	-	1294538..1294834 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PCP88_RS06200 (PCP88_06200)	-	1294831..1295424 (-)	594	WP_003084544.1	YggT family protein	-
PCP88_RS06205 (PCP88_06205)	proC	1295435..1296256 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP88_RS06210 (PCP88_06210)	-	1296268..1296960 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP88_RS06215 (PCP88_06215)	pilT	1297175..1298209 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP88_RS06220 (PCP88_06220)	pilU	1298387..1299535 (+)	1149	WP_003110016.1	type IV pilus ATPase PilU	Machinery gene
PCP88_RS06225 (PCP88_06225)	czcD	1299543..1300442 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP88_RS06230 (PCP88_06230)	-	1300596..1301000 (+)	405	WP_003084560.1	NINE protein	-
PCP88_RS06235 (PCP88_06235)	-	1301243..1302616 (+)	1374	WP_003084562.1	pyridoxal-phosphate dependent enzyme	-
PCP88_RS06240 (PCP88_06240)	-	1302613..1303797 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42559.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=771436 PCP88_RS06220 WP_003110016.1 1298387..1299535(+) (pilU) [Pseudomonas aeruginosa strain F003]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSNLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=771436 PCP88_RS06220 WP_003110016.1 1298387..1299535(+) (pilU) [Pseudomonas aeruginosa strain F003]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAACCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: A0A0H2ZL88

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.22	100	0.882
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382