Detailed information NTG0770408

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP78_RS03050
• Genome accession: NZ_CP115253
• Coordinates: 658077..659225 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F033
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 653077..664225

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP78_RS03025 (PCP78_03025)	-	654228..654524 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
PCP78_RS03030 (PCP78_03030)	-	654521..655114 (-)	594	WP_003084544.1	YggT family protein	-
PCP78_RS03035 (PCP78_03035)	proC	655125..655946 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP78_RS03040 (PCP78_03040)	-	655958..656650 (-)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP78_RS03045 (PCP78_03045)	pilT	656865..657899 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP78_RS03050 (PCP78_03050)	pilU	658077..659225 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PCP78_RS03055 (PCP78_03055)	czcD	659233..660132 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP78_RS03060 (PCP78_03060)	-	660286..660690 (+)	405	WP_003084560.1	NINE protein	-
PCP78_RS03065 (PCP78_03065)	-	660933..662306 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PCP78_RS03070 (PCP78_03070)	-	662303..663487 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=770408 PCP78_RS03050 WP_003100959.1 658077..659225(+) (pilU) [Pseudomonas aeruginosa strain F033]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=770408 PCP78_RS03050 WP_003100959.1 658077..659225(+) (pilU) [Pseudomonas aeruginosa strain F033]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382