Detailed information NTG0770134

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP50_RS01380
• Genome accession: NZ_CP115245
• Coordinates: 284319..285467 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F041
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 279319..290467

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP50_RS01360 (PCP50_01360)	-	280057..281241 (-)	1185	WP_003110017.1	cystathionine gamma-synthase	-
PCP50_RS01365 (PCP50_01365)	-	281238..282611 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PCP50_RS01370 (PCP50_01370)	-	282854..283258 (-)	405	WP_003084560.1	NINE protein	-
PCP50_RS01375 (PCP50_01375)	czcD	283412..284311 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP50_RS01380 (PCP50_01380)	pilU	284319..285467 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PCP50_RS01385 (PCP50_01385)	pilT	285645..286679 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP50_RS01390 (PCP50_01390)	-	286894..287586 (+)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP50_RS01395 (PCP50_01395)	proC	287598..288419 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP50_RS01400 (PCP50_01400)	-	288430..289023 (+)	594	WP_003084544.1	YggT family protein	-
PCP50_RS01405 (PCP50_01405)	-	289020..289316 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=770134 PCP50_RS01380 WP_003100959.1 284319..285467(-) (pilU) [Pseudomonas aeruginosa strain F041]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=770134 PCP50_RS01380 WP_003100959.1 284319..285467(-) (pilU) [Pseudomonas aeruginosa strain F041]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAAACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382