Detailed information NTG0000077

Experimentally validated

Overview

• Name: ydiP
• Type: Auxiliary factor
• Locus tag: BSU_06070
• Genome accession: NC_000964
• Coordinates: 656528..657697 (+)
• Length: 389 a.a.
• NCBI ID: NP_388488.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis str. 168
• Function: digesting foreign DNA Defense system

Function

The frequency of transformation with plasmid pHV1401, which had three XhoI sites, was much lower in the wild-type strain, while it was somewhat higher in mutant strains carrying disrupted ydiO, ydiP, ydiR, ydiS, or ydjA.

Genomic Context

Location: 651528..662697

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BSU_06040 (BSU06040)	ydiM	653432..653812 (+)	381	NP_388485.1	hypothetical protein; prophage 3 region	-
BSU_06048 (BSU06048)	ydzU	654071..654343 (+)	273	YP_003097688.1	hypothetical protein; prophage 3 region	-
BSU_06060 (BSU06060)	ydiO	655223..656506 (+)	1284	NP_388487.1	DNA-methyltransferase (cytosine-specific); prophage 3 region	Auxiliary factor
BSU_06070 (BSU06070)	ydiP	656528..657697 (+)	1170	NP_388488.1	DNA-methyltransferase (cytosine-specific); defective prophage 3	Auxiliary factor
BSU_06090 (BSU06090)	ydiR	659623..660564 (+)	942	NP_388490.1	type-2 restriction enzyme BsuMI component BsuRA (YdiR); prophage region 3	Auxiliary factor
BSU_06100 (BSU06100)	ydiS	660596..661627 (+)	1032	NP_388491.1	type-2 restriction enzyme BsuMI component BsuRB (YdiS); prophage region 3	Auxiliary factor

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 43284.24 Da
• Isoelectric Point: 6.8247

>NTDB_id=77 BSU_06070 NP_388488.1 656528..657697(+) (ydiP) [Bacillus subtilis subsp. subtilis str. 168]
MKVVSLFSGIGGIELGLHQSGHTTEIFCEVDPLAKAVLSKNFPGVKIEDDINEIRELPSCDLVAAGFPCQDLSQAGGKEG
IDGSRSGLVKKLFELIEKKEHANRPPWILIENVPYMLRLNRGKAMSYLTSVLSELGYTWAYRTVDARCFGLPQRRHRVIL
LASLFEDPKDVIFSQDHSEPDLDGKPSVVDHSNYYGFYWTEGLRGVGWAREAVPPIKCGSSVGIASPPAVWSPYEDIVGT
INIRDAERLQGFPEDWTNITTETGKDIKEGARWRLVGNAVSVRVSKWIGENLSQPKGSISDFEGELVTKTWPSAAWGYGD
KKYKVPVSKWVANTEQIAISEFLNHPLKPLSARALNGFLGRAARCTNVNYSDEFINSLERCKDRQLQKV

Nucleotide

• Length: 1170 bp

>NTDB_id=77 BSU_06070 NP_388488.1 656528..657697(+) (ydiP) [Bacillus subtilis subsp. subtilis str. 168]
TTGAAAGTTGTTAGTTTATTCTCCGGCATAGGCGGAATAGAGCTTGGCCTTCATCAGTCAGGACATACAACAGAAATATT
TTGTGAGGTTGATCCTTTGGCAAAAGCTGTTTTATCAAAAAACTTCCCTGGCGTTAAGATAGAAGATGATATTAATGAAA
TTAGAGAGTTACCAAGCTGTGATTTAGTTGCGGCAGGGTTTCCATGCCAAGATTTAAGTCAAGCTGGAGGTAAAGAAGGG
ATTGACGGATCACGCTCCGGTTTAGTTAAGAAACTATTTGAACTTATAGAAAAGAAAGAACATGCAAATCGCCCTCCATG
GATACTAATTGAAAATGTCCCATATATGTTAAGGTTAAACCGTGGTAAAGCTATGTCATATTTAACATCTGTCCTTTCTG
AATTAGGTTATACTTGGGCATATCGAACTGTTGATGCTCGTTGTTTTGGTTTACCGCAACGAAGACATAGAGTTATTTTG
TTAGCCTCTTTATTTGAAGATCCAAAAGATGTGATTTTTTCACAGGATCATTCAGAACCAGATTTAGATGGAAAGCCTTC
TGTTGTGGATCATTCCAACTATTATGGTTTTTATTGGACGGAAGGGTTAAGAGGTGTCGGCTGGGCACGTGAAGCAGTTC
CACCTATAAAATGCGGATCGTCTGTAGGTATAGCCTCTCCACCTGCAGTTTGGTCTCCTTACGAGGATATAGTAGGGACC
ATTAATATAAGGGATGCTGAGCGCCTACAAGGATTCCCGGAAGATTGGACAAATATCACTACTGAAACAGGTAAGGATAT
AAAAGAAGGTGCCAGATGGAGATTGGTGGGAAATGCTGTTTCGGTAAGGGTTTCTAAATGGATTGGTGAAAACTTAAGTC
AGCCAAAAGGGTCGATATCAGATTTTGAAGGTGAATTAGTGACCAAGACTTGGCCAAGTGCAGCATGGGGGTACGGAGAT
AAAAAGTATAAAGTTCCCGTATCAAAATGGGTTGCAAACACAGAACAAATAGCTATTTCAGAGTTTTTAAATCATCCATT
AAAACCTTTATCTGCTAGGGCATTAAACGGTTTTTTAGGTAGAGCAGCCAGGTGTACAAATGTAAATTATTCGGATGAAT
TTATTAACTCGTTAGAACGTTGTAAGGATAGGCAACTTCAAAAGGTTTAA

Domains

Predicted by InterproScan.

• C-5 cytosine-specific DNA methylase (DNA_methylase) (1-170)

• C-5 cytosine-specific DNA methylase (DNA_methylase) (241-293)

References

• [1]: Hideyuki Ohshima et al. (2002) Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg. Journal of Bacteriology 184(2):381-9. [PMID: 11751814]