Detailed information    

experimental Experimentally validated

Overview

Name   ydiS   Type   Auxiliary factor
Locus tag   BSU_06100 Genome accession   NC_000964
Coordinates   660596..661627 (+) Length   343 a.a.
NCBI ID   NP_388491.1    Uniprot ID   A0A6M3Z7Y4
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   digesting foreign DNA   
Defense system

Function

The ratio of the frequency of transformation with the plasmid without an XhoI site to the frequency of transformation with the plasmid with three XhoI sites was more than 10 times higher when the ydiR-ydiS-ydjA operon-disrupted strains were used.


Genomic Context

Location: 655596..666627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_06070 (BSU06070) ydiP 656528..657697 (+) 1170 NP_388488.1 DNA-methyltransferase (cytosine-specific); defective prophage 3 Auxiliary factor
  BSU_06090 (BSU06090) ydiR 659623..660564 (+) 942 NP_388490.1 type-2 restriction enzyme BsuMI component BsuRA (YdiR); prophage region 3 Auxiliary factor
  BSU_06100 (BSU06100) ydiS 660596..661627 (+) 1032 NP_388491.1 type-2 restriction enzyme BsuMI component BsuRB (YdiS); prophage region 3 Auxiliary factor
  BSU_06110 (BSU06110) ydiA 661630..663027 (+) 1398 NP_388492.1 type-2 restriction enzyme BsuMI component BsuRC (YdjA); prophage region 3 Auxiliary factor
  BSU_06120 (BSU06120) ydjB 663601..663936 (+) 336 NP_388493.1 hypothetical protein; prophage region 3 -
  BSU_06130 (BSU06130) ydjC 664319..664669 (+) 351 NP_388494.1 conserved hypothetical protein; prophage region 3 -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 40032.86 Da        Isoelectric Point: 8.7715

>NTDB_id=74 BSU_06100 NP_388491.1 660596..661627(+) (ydiS) [Bacillus subtilis subsp. subtilis str. 168]
MEISKQTSDLLLSLEKKKGTLPKFSVLRSIPRNRIIYGAPGTGKSNYLEREVGKIFGDNPYVFTRVTFFPGYTYGQFIGA
YKPVPIYKKLSGEEEIFSSNFRDKMENFEPMIDYQFVPGPFIDVLIKALKNRYTNFILIIEEINRANAASVFGDIFQLLD
RNKNGESDYPVTFGPDIMNYLARNGIKDEMIKLPSNFFIWATMNNADQGVLPLDTAFKRRWSFEYLELEKYRKAVDSWKL
SLRYKGHNKVIMWNDFRDIINKRLKGKVPEDKLLGPFFLKESELWNQNVFKNKLLYYLKEDVFKHNPTIDFLNASTFSEL
IEKYDGSDNIFTFDIDDSSFVSD

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=74 BSU_06100 NP_388491.1 660596..661627(+) (ydiS) [Bacillus subtilis subsp. subtilis str. 168]
ATGGAAATATCCAAACAAACTTCAGATTTATTATTGAGCCTAGAAAAGAAAAAAGGCACTTTACCAAAATTCAGCGTTTT
ACGCAGTATTCCTAGGAATAGGATTATTTATGGTGCACCAGGAACAGGGAAGAGTAATTATCTAGAAAGGGAAGTGGGAA
AGATCTTTGGTGATAATCCATATGTATTCACCCGAGTTACTTTTTTCCCTGGTTATACATATGGACAATTTATAGGTGCT
TATAAACCGGTTCCTATATATAAAAAATTAAGCGGTGAGGAAGAGATATTTAGTTCAAACTTTAGGGATAAGATGGAAAA
CTTTGAGCCAATGATTGATTATCAATTTGTTCCTGGCCCATTTATCGATGTATTAATTAAAGCTCTGAAAAATAGATACA
CTAATTTTATATTAATTATCGAAGAAATTAATCGTGCCAATGCAGCAAGTGTTTTTGGTGATATTTTTCAATTATTAGAC
AGAAATAAAAACGGTGAAAGTGATTATCCTGTTACATTCGGGCCGGACATTATGAATTATTTAGCGAGAAACGGAATTAA
AGATGAAATGATTAAGCTACCATCCAATTTCTTTATTTGGGCAACTATGAATAATGCGGATCAGGGTGTTTTACCGCTCG
ATACCGCTTTTAAGAGAAGGTGGTCATTTGAATATTTAGAGTTGGAAAAGTATAGAAAGGCAGTGGACTCTTGGAAGCTG
AGCCTCAGATATAAAGGTCATAATAAAGTAATTATGTGGAATGATTTCCGAGACATTATTAATAAGCGTCTTAAAGGAAA
AGTACCAGAGGATAAGCTGCTTGGACCTTTTTTCCTGAAAGAAAGTGAACTGTGGAATCAGAATGTATTTAAAAATAAAC
TGCTTTATTATTTGAAGGAAGATGTTTTCAAGCATAATCCAACGATTGATTTTTTAAATGCTAGTACTTTCTCTGAGCTA
ATTGAAAAATATGATGGCTCGGATAACATTTTTACTTTTGACATAGATGATTCGTCTTTTGTGAGTGATTAA

Domains

Predicted by InterproScan.

AAA domain (dynein-related subfamily) (AAA_5)

(35-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M3Z7Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Hideyuki Ohshima et al. (2002) Molecular organization of intrinsic restriction and modification genes BsuM of Bacillus subtilis Marburg. Journal of Bacteriology 184(2):381-9. [PMID: 11751814]