Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   H7K58_RS02305 Genome accession   NZ_AP022188
Coordinates   489118..490557 (+) Length   479 a.a.
NCBI ID   WP_182787431.1    Uniprot ID   -
Organism   Aeromonas media strain WP7-W18-ESBL-02     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484118..495557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K58_RS02275 (WP7W18E02_04380) mlaF 484696..485496 (-) 801 WP_005331323.1 phospholipid ABC transporter ATP-binding protein MlaF -
  H7K58_RS02280 (WP7W18E02_04390) - 485703..486698 (+) 996 WP_041204878.1 KpsF/GutQ family sugar-phosphate isomerase -
  H7K58_RS02285 (WP7W18E02_04400) kdsC 486698..487252 (+) 555 WP_005331333.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  H7K58_RS02290 (WP7W18E02_04410) lptC 487249..487809 (+) 561 WP_005331338.1 LPS export ABC transporter periplasmic protein LptC -
  H7K58_RS02295 (WP7W18E02_04420) lptA 487796..488314 (+) 519 WP_041204879.1 lipopolysaccharide transport periplasmic protein LptA -
  H7K58_RS02300 (WP7W18E02_04430) lptB 488327..489052 (+) 726 WP_010675872.1 LPS export ABC transporter ATP-binding protein -
  H7K58_RS02305 (WP7W18E02_04440) rpoN/rpoN1 489118..490557 (+) 1440 WP_182787431.1 RNA polymerase factor sigma-54 Machinery gene
  H7K58_RS02310 (WP7W18E02_04450) hpf 490578..490865 (+) 288 WP_005305957.1 ribosome hibernation promoting factor -
  H7K58_RS02315 (WP7W18E02_04460) ptsN 490868..491314 (+) 447 WP_010675870.1 PTS IIA-like nitrogen regulatory protein PtsN -
  H7K58_RS02320 (WP7W18E02_04470) rapZ 491355..492221 (+) 867 WP_182787433.1 RNase adapter RapZ -
  H7K58_RS02325 (WP7W18E02_04480) npr 492242..492514 (+) 273 WP_005331359.1 PTS phosphocarrier protein NPr -
  H7K58_RS02330 - 492533..493592 (-) 1060 Protein_443 PTS sugar transporter subunit IIA -
  H7K58_RS02335 (WP7W18E02_04520) degS 493695..494816 (-) 1122 WP_041204883.1 outer membrane-stress sensor serine endopeptidase DegS -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53787.80 Da        Isoelectric Point: 4.4457

>NTDB_id=76996 H7K58_RS02305 WP_182787431.1 489118..490557(+) (rpoN/rpoN1) [Aeromonas media strain WP7-W18-ESBL-02]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAHETSPIEPQDASQLDSCDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGAKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=76996 H7K58_RS02305 WP_182787431.1 489118..490557(+) (rpoN/rpoN1) [Aeromonas media strain WP7-W18-ESBL-02]
ATGAAGCCGACATTACAGTTGCGTCTCGGCCAGTCCCTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCACGAAACCAGTCCGATAGAACCCCAGGACGCCAGTCAACTCGACTCCTGTGACGCAATGGCACAGGACAAGATGCCG
GATGAACTGCCGGTGGACACTACCTGGGATGAGGTCTATTCCGCCGGCACGGCTCAGAGCGCTGGCCCCATCCATGACGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAAAATCTGCAGGATTATCTGTTGTGGCAGATGCGACTGACGCCGTTCA
GTGATCTGGATGCCGCGATTGCCCTCGCCATCATCGATGCCATCGATGAGTCTGGCTACCTGACCGTTTCTCTCGAAGAT
ATTCAGGCTGCAGTCAGCAGTGAAGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTTGGCATCGCCGCTCGCTCGGTACAGGAGTGTTTGTTGATCCAGCTTGGCCAGTATTCGCCGGATCTGCCCT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGACTTGCTCGGCAACCGTGACTACCGCACCTTGGCGCGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTACTGGATCTCATCCAGCAGCTGGAGCCTAGACCCGGTAATGGCATCAT
CCAGAGCGAATCGCAATACGTGATCCCGGATGTCTCCGTGGTCAAGAAGGGGGCGAAGTGGGTGGTTGAACTCAACCCGG
ATTCGGCGCCGCGCATTCGTGTCAACGAGGCCTATGCGGCAATGGCACGCAATGCCCGCAGCAGCACGGATACTCAGTTC
ATTCGTTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGAAACGACACCCTGCTCAAGGTGGCAAG
CTGCATAGTCGAGCAGCAACAGGGTTTCTTCGAGTACGGTGAAGAGGCCATGAAGCCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGTGCCCTGATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.063

100

0.489

Multiple sequence alignment