Detailed information NTG0769862

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PCP25_RS04240
• Genome accession: NZ_CP115233
• Coordinates: 856380..857528 (-)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain F049
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 851380..862528

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PCP25_RS04220 (PCP25_04225)	-	852118..853302 (-)	1185	WP_003100953.1	cystathionine gamma-synthase	-
PCP25_RS04225 (PCP25_04230)	-	853299..854672 (-)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
PCP25_RS04230 (PCP25_04235)	-	854915..855319 (-)	405	WP_003084560.1	NINE protein	-
PCP25_RS04235 (PCP25_04240)	czcD	855473..856372 (+)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
PCP25_RS04240 (PCP25_04245)	pilU	856380..857528 (-)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
PCP25_RS04245 (PCP25_04250)	pilT	857706..858740 (-)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
PCP25_RS04250 (PCP25_04255)	-	858955..859647 (+)	693	WP_010793543.1	YggS family pyridoxal phosphate-dependent enzyme	-
PCP25_RS04255 (PCP25_04260)	proC	859659..860480 (+)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
PCP25_RS04260 (PCP25_04265)	-	860491..861084 (+)	594	WP_003084544.1	YggT family protein	-
PCP25_RS04265 (PCP25_04270)	-	861081..861377 (+)	297	WP_031628111.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=769862 PCP25_RS04240 WP_003100959.1 856380..857528(-) (pilU) [Pseudomonas aeruginosa strain F049]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=769862 PCP25_RS04240 WP_003100959.1 856380..857528(-) (pilU) [Pseudomonas aeruginosa strain F049]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382