Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   LPH31_RS01895 Genome accession   NZ_AP025220
Coordinates   411757..412434 (-) Length   225 a.a.
NCBI ID   WP_229430222.1    Uniprot ID   -
Organism   Escherichia coli strain 2017.11.01CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 406757..417434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPH31_RS01880 (VEGS20_A03660) chiA 408124..410817 (+) 2694 WP_104774102.1 bifunctional chitinase/lysozyme -
  LPH31_RS01885 (VEGS20_A03670) bfd 410986..411180 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  LPH31_RS01890 (VEGS20_A03680) bfr 411252..411728 (+) 477 WP_000675504.1 bacterioferritin -
  LPH31_RS01895 (VEGS20_A03690) pilD 411757..412434 (-) 678 WP_229430222.1 prepilin peptidase GspO Machinery gene
  LPH31_RS01900 (VEGS20_A03700) gspM 412434..412895 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  LPH31_RS01905 (VEGS20_A03710) gspL 412892..414055 (-) 1164 WP_072778696.1 type II secretion system protein GspL -
  LPH31_RS01910 (VEGS20_A03720) gspK 414070..415053 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  LPH31_RS01915 (VEGS20_A03730) gspJ 415046..415634 (-) 589 Protein_380 type II secretion system minor pseudopilin GspJ -
  LPH31_RS01920 (VEGS20_A03740) gspI 415627..416004 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  LPH31_RS01925 (VEGS20_A03750) gspH 416001..416510 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  LPH31_RS01930 (VEGS20_A03760) gspG 416518..416955 (-) 438 WP_077250035.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24902.73 Da        Isoelectric Point: 7.6352

>NTDB_id=76975 LPH31_RS01895 WP_229430222.1 411757..412434(-) (pilD) [Escherichia coli strain 2017.11.01CC]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYASVSQLLQRWTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=76975 LPH31_RS01895 WP_229430222.1 411757..412434(-) (pilD) [Escherichia coli strain 2017.11.01CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCAGCGTTTCACAACTTCTGCAACGCTGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.171

100

0.369

Multiple sequence alignment