Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QQA21_RS20595 Genome accession   NZ_CP126955
Coordinates   4165764..4166441 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli O78:H4 strain APEC E123     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4160764..4171441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQA21_RS20560 (QQA21_20560) gspG 4161244..4161681 (+) 438 WP_001202880.1 type II secretion system major pseudopilin GspG -
  QQA21_RS20565 (QQA21_20565) gspH 4161689..4162198 (+) 510 WP_001076048.1 type II secretion system minor pseudopilin GspH -
  QQA21_RS20570 (QQA21_20570) gspI 4162195..4162572 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  QQA21_RS20575 (QQA21_20575) gspJ 4162565..4163152 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  QQA21_RS20580 (QQA21_20580) gspK 4163145..4164128 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  QQA21_RS20585 (QQA21_20585) gspL 4164143..4165306 (+) 1164 WP_001115230.1 type II secretion system protein GspL -
  QQA21_RS20590 (QQA21_20590) gspM 4165303..4165764 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  QQA21_RS20595 (QQA21_20595) pilD 4165764..4166441 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  QQA21_RS20600 (QQA21_20600) bfr 4166470..4166946 (-) 477 WP_000675504.1 bacterioferritin -
  QQA21_RS20605 (QQA21_20605) bfd 4167018..4167212 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QQA21_RS20610 (QQA21_20610) chiA 4167381..4170074 (-) 2694 WP_000773151.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=769461 QQA21_RS20595 WP_000178161.1 4165764..4166441(+) (pilD) [Escherichia coli O78:H4 strain APEC E123]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=769461 QQA21_RS20595 WP_000178161.1 4165764..4166441(+) (pilD) [Escherichia coli O78:H4 strain APEC E123]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378