Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   LPH32_RS16600 Genome accession   NZ_AP025214
Coordinates   3413497..3414174 (-) Length   225 a.a.
NCBI ID   WP_000178169.1    Uniprot ID   -
Organism   Escherichia coli strain 2017.09.02CC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3408497..3419174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPH32_RS16585 (VEGS19_A31530) chiA 3409855..3412557 (+) 2703 WP_000773176.1 bifunctional chitinase/lysozyme -
  LPH32_RS16590 (VEGS19_A31540) bfd 3412726..3412920 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  LPH32_RS16595 (VEGS19_A31550) bfr 3412992..3413468 (+) 477 WP_000675505.1 bacterioferritin -
  LPH32_RS16600 (VEGS19_A31560) pilD 3413497..3414174 (-) 678 WP_000178169.1 prepilin peptidase GspO Machinery gene
  LPH32_RS16605 (VEGS19_A31570) gspM 3414174..3414635 (-) 462 WP_000597914.1 type II secretion system protein GspM -
  LPH32_RS16610 (VEGS19_A31580) gspL 3414632..3415795 (-) 1164 WP_001115245.1 type II secretion system protein GspL -
  LPH32_RS16615 (VEGS19_A31590) gspK 3415810..3416793 (-) 984 WP_001058528.1 type II secretion system minor pseudopilin GspK -
  LPH32_RS16620 (VEGS19_A31600) gspJ 3416786..3417373 (-) 588 WP_000610629.1 type II secretion system minor pseudopilin GspJ -
  LPH32_RS16625 (VEGS19_A31610) gspI 3417366..3417743 (-) 378 WP_001041738.1 type II secretion system minor pseudopilin GspI -
  LPH32_RS16630 (VEGS19_A31620) gspH 3417740..3418249 (-) 510 WP_001076031.1 type II secretion system minor pseudopilin GspH -
  LPH32_RS16635 (VEGS19_A31630) gspG 3418257..3418694 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24812.67 Da        Isoelectric Point: 7.9585

>NTDB_id=76943 LPH32_RS16600 WP_000178169.1 3413497..3414174(-) (pilD) [Escherichia coli strain 2017.09.02CC]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=76943 LPH32_RS16600 WP_000178169.1 3413497..3414174(-) (pilD) [Escherichia coli strain 2017.09.02CC]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTAATTGCCTCACTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCGTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.382

Multiple sequence alignment