Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PCQ86_RS16480 Genome accession   NZ_CP115182
Coordinates   3394218..3395366 (+) Length   382 a.a.
NCBI ID   WP_196995883.1    Uniprot ID   -
Organism   Diaphorobacter sp. ED-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3389218..3400366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCQ86_RS16460 coaD 3390073..3390570 (-) 498 WP_270174230.1 pantetheine-phosphate adenylyltransferase -
  PCQ86_RS16465 rsmD 3390716..3391339 (-) 624 WP_270174231.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  PCQ86_RS16470 - 3391336..3392718 (-) 1383 WP_270174232.1 pitrilysin family protein -
  PCQ86_RS16475 - 3392734..3394188 (-) 1455 WP_220637910.1 pitrilysin family protein -
  PCQ86_RS16480 pilA 3394218..3395366 (+) 1149 WP_196995883.1 signal recognition particle-docking protein FtsY Machinery gene
  PCQ86_RS16485 cutA 3395417..3395779 (-) 363 WP_270174233.1 divalent-cation tolerance protein CutA -
  PCQ86_RS16490 rpoH 3395970..3396905 (+) 936 WP_270174234.1 RNA polymerase sigma factor RpoH -
  PCQ86_RS16495 - 3397032..3398042 (+) 1011 WP_270174235.1 tripartite tricarboxylate transporter substrate binding protein -
  PCQ86_RS16500 - 3398290..3399072 (+) 783 WP_270174236.1 Crp/Fnr family transcriptional regulator -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 39755.51 Da        Isoelectric Point: 6.0008

>NTDB_id=768686 PCQ86_RS16480 WP_196995883.1 3394218..3395366(+) (pilA) [Diaphorobacter sp. ED-3]
MFSFFKKKSPAPAEPALAPEDVASPAAPPAADAPASGGAFGWLRKPFGAGATSVAEPAAPSAGAPAAIDVPLAVPEPTPK
PGGARQGWMDRLKSGLRKTGSSIATVFTGTQINDALYDELEEALLMADTGVKATQHLLEDLKRRVKETKTTEPAAVKGLL
ADALADLLRPLEKPLVIGEHTPTVIMVAGVNGAGKTTSIGKLTKHLADHGESVLLAAADTFRAAAREQLGVWATRNTVEI
VSQEGGDPAAVSFDAVTAGRARGKDVVLVDTAGRLPTQLHLMEELKKIRRVVTKADASAPHEVLLVIDGNTGQNALAQVR
AFDDALQLTGLIVTKLDGTAKGGVLAAIAQERPVPVYFIGVGEKVEDLETFSAREFAQALLT

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=768686 PCQ86_RS16480 WP_196995883.1 3394218..3395366(+) (pilA) [Diaphorobacter sp. ED-3]
ATGTTCAGTTTTTTCAAGAAAAAATCGCCCGCCCCCGCCGAACCCGCCCTTGCACCCGAGGACGTGGCCAGCCCTGCGGC
GCCGCCCGCGGCCGACGCCCCCGCCAGTGGCGGAGCATTCGGCTGGCTGCGCAAGCCCTTTGGCGCAGGCGCCACCAGCG
TGGCGGAGCCGGCTGCCCCTTCGGCCGGCGCCCCCGCAGCCATCGACGTGCCCCTTGCCGTCCCCGAGCCCACCCCGAAG
CCCGGGGGCGCACGCCAGGGCTGGATGGACCGGCTGAAATCCGGCCTGCGCAAGACCGGCAGCAGCATCGCGACCGTCTT
CACGGGCACGCAGATCAACGACGCGCTGTACGACGAACTGGAGGAAGCCCTGCTCATGGCCGACACCGGGGTGAAAGCCA
CACAGCACCTGCTGGAGGACCTCAAGCGCCGCGTGAAAGAGACCAAGACCACCGAGCCTGCTGCCGTAAAGGGACTGCTG
GCCGATGCGCTGGCCGACCTGCTGCGGCCGCTGGAAAAGCCGCTGGTGATCGGCGAGCACACACCCACCGTGATCATGGT
GGCCGGCGTGAACGGCGCAGGCAAGACCACCTCCATCGGCAAGCTCACCAAGCACCTGGCCGACCATGGCGAGAGCGTGC
TGCTGGCCGCCGCCGATACCTTCCGCGCCGCGGCGCGCGAGCAGTTGGGTGTCTGGGCCACGCGCAACACGGTGGAGATC
GTCAGCCAGGAAGGGGGCGACCCTGCCGCCGTGAGCTTTGACGCCGTTACCGCCGGCCGCGCGCGCGGCAAAGATGTGGT
GCTGGTGGACACCGCCGGCCGCCTGCCTACGCAGCTGCACCTGATGGAAGAGCTGAAGAAGATCCGCCGCGTGGTCACCA
AGGCCGATGCCAGCGCCCCGCACGAGGTGCTACTGGTGATCGACGGCAACACCGGCCAGAACGCCCTGGCGCAGGTGCGC
GCGTTCGACGACGCGCTTCAACTCACCGGCCTGATCGTCACCAAACTGGACGGCACGGCCAAGGGCGGCGTGCTGGCCGC
CATCGCGCAGGAGCGGCCCGTGCCCGTGTACTTCATTGGCGTGGGCGAGAAAGTGGAAGACCTGGAGACCTTCAGCGCAC
GCGAATTCGCCCAGGCCCTGCTGACCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(182-381)

SRP54-type protein, helical bundle domain (SRP54_N)

(101-164)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.24

100

0.547