Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PCQ86_RS02730 Genome accession   NZ_CP115182
Coordinates   553839..555416 (+) Length   525 a.a.
NCBI ID   WP_270174813.1    Uniprot ID   -
Organism   Diaphorobacter sp. ED-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 548839..560416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCQ86_RS02705 argS 549126..550829 (+) 1704 WP_270174812.1 arginine--tRNA ligase -
  PCQ86_RS02710 - 550842..551468 (+) 627 WP_047349343.1 SPOR domain-containing protein -
  PCQ86_RS02715 - 551527..552177 (+) 651 WP_011803808.1 thiol:disulfide interchange protein DsbA/DsbL -
  PCQ86_RS02720 lptA 552351..553010 (+) 660 WP_041835854.1 lipopolysaccharide transport periplasmic protein LptA -
  PCQ86_RS02725 lptB 553034..553786 (+) 753 WP_012655406.1 LPS export ABC transporter ATP-binding protein -
  PCQ86_RS02730 rpoN/rpoN1 553839..555416 (+) 1578 WP_270174813.1 RNA polymerase factor sigma-54 Machinery gene
  PCQ86_RS02735 - 555420..555863 (+) 444 WP_012655408.1 thioesterase family protein -
  PCQ86_RS02740 - 555860..557173 (+) 1314 WP_270174814.1 THUMP domain-containing protein -
  PCQ86_RS02745 - 557163..557648 (+) 486 WP_270174815.1 putative toxin-antitoxin system toxin component, PIN family -
  PCQ86_RS02750 - 557636..558937 (+) 1302 WP_011803815.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  PCQ86_RS02755 - 559420..560250 (+) 831 WP_270174816.1 UPF0149 family protein -

Sequence

Protein


Download         Length: 525 a.a.        Molecular weight: 57463.56 Da        Isoelectric Point: 4.5772

>NTDB_id=768674 PCQ86_RS02730 WP_270174813.1 553839..555416(+) (rpoN/rpoN1) [Diaphorobacter sp. ED-3]
MKPGLSLRVSQHLALTPQLQQSIRLLQLSTLELAQEVEQMLDENPFLERTLEEAPREEFGLAQADAPVSEQDREVEDAIY
SGAVSAGQESATSQNDAETSTAESTAEPADWEGDGSVDLAPDDSEWGGDAPARNGAGAGSDDEVDATELARSQESLTAFL
HRQALALRLSELDMAALRFLIESLNDDGYLDESLQDLAASLAGTEDPEQLDELVHRFTVALRLLQSLEPAGVGARGLAEC
LTLQLRAMDQDEEGDPDVIQAALAICGQPLDLLAKRDVRRLAQACGTSEERTRAAMALIARLEPRPGRRFADVDRNIIVP
DVIVKKTGRGGQQNFSVQLNPDVMPRLRVHDIYAGALRGGRGEGHQALQARLQEARWFIKNIQQRFDTILRVSRAIVERQ
KNFFVHGELAMRPLVLRDIADELGLHESTISRVTTAKYMATPQGTYELKYFFGSGLGTETGGNASSTAVRALIKQFIAAE
NPHKPLSDSQIAEMLKEQGIECARRTVAKYREALKIAPANLRKAL

Nucleotide


Download         Length: 1578 bp        

>NTDB_id=768674 PCQ86_RS02730 WP_270174813.1 553839..555416(+) (rpoN/rpoN1) [Diaphorobacter sp. ED-3]
ATGAAGCCCGGCCTGTCGCTGCGCGTCTCGCAGCACCTGGCGCTCACACCGCAGTTGCAGCAGTCGATCCGCCTGCTGCA
GCTGTCCACGCTGGAGCTGGCCCAGGAGGTCGAGCAAATGCTCGACGAGAACCCCTTCCTGGAGCGCACGCTGGAAGAGG
CCCCGCGCGAGGAGTTCGGCCTGGCCCAGGCCGATGCACCGGTGAGCGAGCAAGACCGAGAAGTCGAAGATGCTATTTAT
TCAGGAGCTGTCAGCGCAGGGCAGGAAAGCGCTACAAGCCAAAATGATGCAGAAACCTCCACAGCCGAATCCACGGCCGA
ACCCGCGGACTGGGAAGGCGACGGCTCGGTAGACCTGGCGCCCGACGACAGCGAGTGGGGCGGCGACGCCCCGGCACGCA
ACGGTGCCGGCGCGGGGAGCGACGACGAGGTGGACGCCACCGAGCTGGCCCGCAGCCAGGAGTCGCTCACGGCCTTCCTG
CACCGCCAGGCGCTGGCGCTGCGCCTGTCCGAGCTGGACATGGCCGCGCTGCGCTTCCTCATCGAATCGCTGAACGACGA
CGGCTACCTGGACGAGTCGCTGCAGGACCTGGCCGCCAGCCTGGCGGGCACCGAAGACCCCGAGCAACTCGATGAATTGG
TGCACCGCTTCACCGTGGCGCTGCGCCTGCTGCAAAGCCTGGAGCCCGCGGGCGTGGGCGCGCGCGGACTGGCCGAATGC
CTCACGCTGCAGTTGCGTGCGATGGACCAGGACGAAGAGGGCGACCCCGACGTGATCCAGGCCGCGCTGGCCATTTGCGG
CCAGCCGCTGGACCTGCTGGCCAAGCGCGACGTGCGCCGCCTGGCGCAGGCCTGCGGCACCAGCGAAGAGCGCACGCGCG
CTGCGATGGCCTTGATCGCGCGGCTGGAGCCACGCCCGGGCAGGCGCTTTGCCGACGTGGACCGCAACATCATCGTGCCC
GACGTGATCGTCAAGAAAACCGGCCGCGGCGGACAGCAGAACTTCAGCGTGCAGCTCAACCCGGACGTGATGCCGCGCCT
GCGCGTGCACGACATCTACGCCGGCGCGTTGCGCGGCGGCCGTGGCGAAGGCCACCAGGCGCTGCAGGCACGGCTGCAGG
AGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGTTTTGACACCATCCTGCGCGTGTCGCGCGCCATCGTGGAGCGGCAG
AAGAACTTCTTCGTGCATGGCGAACTGGCCATGCGCCCGCTGGTGCTGCGCGACATTGCCGATGAACTGGGCCTGCACGA
GTCCACCATCAGCCGCGTGACCACCGCCAAGTACATGGCCACACCCCAGGGCACGTACGAGCTCAAATACTTCTTCGGCT
CGGGGCTGGGCACCGAAACCGGCGGCAACGCCTCCAGTACGGCCGTGCGCGCGCTCATCAAGCAGTTCATCGCGGCCGAG
AACCCGCACAAGCCGCTGTCGGACAGCCAGATCGCCGAGATGCTCAAGGAGCAGGGCATTGAATGTGCGCGCCGCACCGT
GGCCAAATACCGTGAGGCGCTCAAGATCGCCCCGGCCAACCTCCGCAAGGCACTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(367-523)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(147-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.93

100

0.533