Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   QQA32_RS02205 Genome accession   NZ_CP126926
Coordinates   465586..466263 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli O2:K1:H5 strain APEC E19035     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 460586..471263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QQA32_RS02190 (QQA32_02190) chiA 461944..464646 (+) 2703 WP_000773166.1 bifunctional chitinase/lysozyme -
  QQA32_RS02195 (QQA32_02195) bfd 464815..465009 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  QQA32_RS02200 (QQA32_02200) bfr 465081..465557 (+) 477 WP_000675504.1 bacterioferritin -
  QQA32_RS02205 (QQA32_02205) pilD 465586..466263 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  QQA32_RS02210 (QQA32_02210) gspM 466263..466724 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  QQA32_RS02215 (QQA32_02215) gspL 466721..467884 (-) 1164 WP_001332436.1 type II secretion system protein GspL -
  QQA32_RS02220 (QQA32_02220) gspK 467899..468882 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  QQA32_RS02225 (QQA32_02225) gspJ 468875..469462 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  QQA32_RS02230 (QQA32_02230) gspI 469455..469832 (-) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  QQA32_RS02235 (QQA32_02235) gspH 469829..470338 (-) 510 WP_001076038.1 type II secretion system minor pseudopilin GspH -
  QQA32_RS02240 (QQA32_02240) gspG 470346..470783 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=768481 QQA32_RS02205 WP_000178175.1 465586..466263(-) (pilD) [Escherichia coli O2:K1:H5 strain APEC E19035]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=768481 QQA32_RS02205 WP_000178175.1 465586..466263(-) (pilD) [Escherichia coli O2:K1:H5 strain APEC E19035]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387