Detailed information NTG0768127

Bioinformatically predicted

Overview

• Name: hexA
• Type: Machinery gene
• Locus tag: LLE01_RS12945
• Genome accession: NZ_CP126675
• Coordinates: 2363368..2365890 (-)
• Length: 840 a.a.
• NCBI ID: WP_010906385.1
• Uniprot ID: Q9CDK9
• Organism: Lactococcus lactis subsp. lactis strain E.0.1
• Function: DNA mismatch repair (predicted from homology) Homologous recombination

Genomic Context

Location: 2358368..2370890

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLE01_RS12925 (LLE01_12895)	ruvB	2358626..2359627 (-)	1002	WP_003131994.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
LLE01_RS12930 (LLE01_12900)	ruvA	2359753..2360346 (-)	594	WP_003131995.1	Holliday junction branch migration protein RuvA	Machinery gene
LLE01_RS12935 (LLE01_12905)	hexB	2360459..2362429 (-)	1971	WP_031297070.1	DNA mismatch repair endonuclease MutL	Machinery gene
LLE01_RS12940 (LLE01_12910)	-	2362558..2363253 (-)	696	WP_010906384.1	hypothetical protein	-
LLE01_RS12945 (LLE01_12915)	hexA	2363368..2365890 (-)	2523	WP_010906385.1	DNA mismatch repair protein MutS	Machinery gene
LLE01_RS12950 (LLE01_12920)	-	2366084..2366431 (-)	348	WP_010906386.1	RicAFT regulatory complex protein RicA family protein	-
LLE01_RS12955 (LLE01_12925)	-	2366800..2367102 (-)	303	WP_161908452.1	helix-turn-helix domain-containing protein	-
LLE01_RS12960 (LLE01_12930)	-	2367235..2368188 (+)	954	WP_010906389.1	hypothetical protein	-
LLE01_RS12965 (LLE01_12935)	-	2368371..2369252 (-)	882	WP_010906390.1	NAD(P)-dependent oxidoreductase	-
LLE01_RS12970 (LLE01_12940)	-	2369294..2369500 (-)	207	WP_003132002.1	hypothetical protein	-
LLE01_RS12980 (LLE01_12950)	-	2369859..2370236 (-)	378	WP_003132003.1	DUF1634 domain-containing protein	-

Sequence

Protein

• Length: 840 a.a.
• Molecular weight: 94272.26 Da
• Isoelectric Point: 4.6787

>NTDB_id=768127 LLE01_RS12945 WP_010906385.1 2363368..2365890(-) (hexA) [Lactococcus lactis subsp. lactis strain E.0.1]
MAEKISPGMQQYLDIKQDYPDAFLLFRMGDFYELFYDDAVNAAQILELTLTSRNKNSENPIPMAGVPHHAAAEYIDKLVD
LGYKVAVAEQMEDPKKAVGIVKRAVTQVITPGTTIDTANSVDNNFLVAIDFKDKHYALSYMDLSTGEFKVTELSEFSAVV
GEIASLKAREIVAGFTLDESQLKVFERQMNLLISEQLEIPENLLIDLSGLTALENQVASKLLAYVKETQMRDLSHLQEVE
HYEIKDFLQMDFATKSSLELTANKRENKKHGTLYWLLDETKTAMGTRMLRSWIDRPLISNSAIQKRMEIVQIFLDHFFER
SDLIEALKGVYDLERLASRVSFGKAVPVDFLQLANSLSNVPAIKYILGMLNEAPLEELKSQLDEIPELSGLINSAISENA
SRTITEGGIIKKGYNAQLDKYREALENGTSWIAKLEADEKAKTGISTLRIDYNRKDGYYFHITQSQLNSVPEHFYRKATL
KNSERFGSQELTEIEEIMLEAREKSSSLEYDLFMGLRTETEQYIGRLQALAKTIAEIDCLQSLSVVAEKQGYIRPTLTDG
SRIVEIKGGRHAVVEAVMGAQEYVPNDIELPEQTDIQLITGPNMSGKSTYMRQFALTVIMAQIGSFVPAETANLPIFDAI
FTRIGASDNLISGESTFMVEMSEANHAIQKASSRSLIIFDELGRGTATYDGMALAQAIIEYVHDHIGAKTLFATHYHELT
DLDEALDHLDNVHVATLEQNGNVTFLHKITEGPADKSYGIHVAKIAGLPQPLLERADLILQKLENKPLPAKKVADEQEQL
SLFDFTENSSEIIEKIKRQNVDNMTAREALNFLWELKDSL

Nucleotide

• Length: 2523 bp

>NTDB_id=768127 LLE01_RS12945 WP_010906385.1 2363368..2365890(-) (hexA) [Lactococcus lactis subsp. lactis strain E.0.1]
ATGGCTGAAAAAATTTCACCAGGAATGCAACAATATTTAGACATCAAACAAGATTATCCCGATGCTTTCTTGCTCTTTCG
GATGGGTGATTTTTATGAGCTTTTCTATGATGATGCAGTGAATGCTGCCCAAATATTAGAATTAACCCTGACTTCAAGAA
ATAAAAATTCAGAAAATCCAATTCCAATGGCTGGTGTTCCTCACCATGCAGCAGCTGAATACATTGATAAATTGGTTGAT
TTAGGCTACAAAGTGGCTGTTGCTGAACAAATGGAAGACCCGAAAAAAGCGGTCGGAATTGTTAAACGGGCAGTAACACA
GGTGATTACCCCAGGGACAACGATTGACACTGCAAATTCGGTTGATAATAATTTCTTAGTGGCAATTGACTTCAAAGATA
AACATTATGCTCTTTCCTATATGGACTTATCAACTGGGGAATTCAAGGTGACAGAGCTCAGCGAATTTTCAGCGGTGGTG
GGAGAAATTGCTTCACTCAAGGCGCGGGAAATTGTAGCTGGTTTTACTTTAGATGAAAGTCAGTTAAAAGTTTTTGAAAG
ACAAATGAATCTTTTGATTTCAGAACAACTTGAAATTCCTGAAAATTTACTGATAGATTTGTCAGGATTGACCGCCTTAG
AAAATCAAGTCGCTTCAAAATTGTTGGCTTATGTCAAAGAAACACAAATGCGTGATTTGAGTCACTTACAAGAAGTTGAA
CATTATGAAATTAAAGACTTTTTGCAAATGGACTTTGCGACAAAATCATCTTTAGAGCTGACGGCAAATAAAAGAGAAAA
TAAAAAACATGGCACACTTTATTGGTTGCTTGATGAGACGAAAACAGCGATGGGAACCAGAATGTTACGGTCGTGGATTG
ACCGTCCTTTGATTTCAAATTCAGCCATTCAAAAACGAATGGAAATTGTTCAAATTTTCTTAGACCATTTCTTTGAGCGT
TCCGATTTGATAGAAGCCTTAAAAGGGGTCTATGATTTAGAACGTTTGGCTTCACGTGTTTCTTTTGGTAAGGCTGTTCC
GGTTGATTTTTTGCAGCTGGCTAATTCTTTATCAAATGTTCCTGCTATCAAATATATTTTAGGAATGCTTAATGAAGCTC
CATTAGAAGAGTTAAAAAGCCAATTAGACGAAATTCCAGAATTATCTGGTTTAATTAATTCAGCTATTAGTGAGAATGCC
TCAAGAACGATTACTGAAGGGGGCATTATTAAAAAAGGCTACAATGCTCAATTAGATAAATATCGGGAGGCGCTTGAAAA
TGGGACTTCTTGGATTGCAAAATTGGAAGCAGATGAAAAAGCCAAGACAGGAATTTCCACACTACGAATTGATTATAATC
GTAAGGATGGTTATTATTTTCATATTACTCAAAGTCAGTTGAATAGTGTTCCTGAACATTTTTATCGGAAGGCAACTTTG
AAAAATTCTGAGCGTTTTGGTTCTCAGGAATTAACTGAAATTGAAGAAATTATGTTGGAAGCGCGTGAAAAATCATCATC
TCTAGAATATGATTTATTCATGGGCCTTCGGACTGAAACGGAGCAATATATTGGCAGACTACAAGCTCTAGCCAAAACAA
TTGCTGAAATTGATTGTTTACAAAGTCTATCTGTCGTTGCCGAAAAACAAGGATATATCCGTCCGACGCTTACAGACGGT
TCAAGAATTGTTGAAATTAAAGGCGGTCGTCATGCGGTCGTTGAAGCGGTCATGGGTGCTCAAGAATATGTGCCAAATGA
TATTGAACTGCCTGAACAAACAGATATTCAACTCATCACAGGACCGAATATGTCAGGTAAATCAACCTACATGCGTCAAT
TTGCTTTGACGGTGATTATGGCTCAAATTGGATCTTTTGTTCCAGCAGAAACAGCCAATTTACCAATTTTTGATGCCATC
TTTACACGGATTGGTGCCAGTGATAACTTGATTTCAGGGGAATCGACCTTTATGGTTGAAATGTCTGAGGCCAATCATGC
CATTCAAAAGGCAAGCAGTCGCAGCCTGATTATTTTTGATGAATTAGGGCGAGGAACGGCAACTTATGATGGGATGGCTC
TAGCTCAAGCAATTATTGAATATGTTCATGACCATATTGGGGCAAAGACCTTGTTTGCTACGCATTATCATGAATTGACA
GACTTAGATGAAGCGCTCGATCATCTTGATAATGTCCATGTTGCTACCTTGGAGCAAAATGGGAATGTGACTTTTTTGCA
TAAAATAACAGAGGGGCCAGCTGATAAATCCTATGGGATTCATGTGGCGAAAATTGCAGGTTTACCACAGCCTTTGTTAG
AGCGAGCAGATTTGATTTTGCAGAAGTTAGAAAATAAACCTTTGCCTGCTAAAAAAGTTGCTGATGAACAAGAACAACTT
AGTTTATTTGATTTTACCGAAAATTCTTCAGAAATTATTGAAAAGATAAAAAGGCAAAATGTTGATAATATGACTGCAAG
AGAGGCATTGAATTTCTTGTGGGAACTTAAAGATTCACTTTGA

Domains

Predicted by InterProScan.

• MutS domain II (MutS_II) (124-237)

• MutS domain I (MutS_I) (7-116)

• MutS family domain IV (MutS_IV) (413-503)

• MutS domain III (MutS_III) (254-544)

• MutS domain V (MutS_V) (598-784)

3D structure

• AlphaFold DB: Q9CDK9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
hexA	Streptococcus pneumoniae R6	64.573	100	0.649
mutS	Pseudomonas stutzeri strain ATCC 17587	37.806	100	0.386