Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H7R61_RS20815 Genome accession   NZ_AP022126
Coordinates   4282065..4283045 (-) Length   326 a.a.
NCBI ID   WP_023337759.1    Uniprot ID   A0A2J0PIG5
Organism   Enterobacter cloacae strain WP5-S18-CRE-02     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4277065..4288045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R61_RS20785 (WP5S18C02_40810) - 4277954..4278451 (+) 498 WP_014885186.1 SprT family zinc-dependent metalloprotease -
  H7R61_RS20790 (WP5S18C02_40820) endA 4278546..4279253 (+) 708 WP_014885187.1 deoxyribonuclease I -
  H7R61_RS20795 (WP5S18C02_40830) rsmE 4279305..4280036 (+) 732 WP_023337758.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H7R61_RS20800 (WP5S18C02_40840) gshB 4280056..4281003 (+) 948 WP_023331278.1 glutathione synthase -
  H7R61_RS20805 (WP5S18C02_40850) - 4281078..4281638 (+) 561 WP_047363978.1 YqgE/AlgH family protein -
  H7R61_RS20810 (WP5S18C02_40860) ruvX 4281638..4282054 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  H7R61_RS20815 (WP5S18C02_40870) pilT 4282065..4283045 (-) 981 WP_023337759.1 type IV pilus twitching motility protein PilT Machinery gene
  H7R61_RS20820 (WP5S18C02_40880) - 4283063..4283764 (+) 702 WP_023337760.1 YggS family pyridoxal phosphate-dependent enzyme -
  H7R61_RS20825 (WP5S18C02_40890) - 4283786..4284352 (+) 567 WP_014885192.1 YggT family protein -
  H7R61_RS20830 (WP5S18C02_40900) yggU 4284349..4284645 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  H7R61_RS20835 (WP5S18C02_40910) - 4284649..4285242 (+) 594 WP_023331280.1 XTP/dITP diphosphatase -
  H7R61_RS20840 (WP5S18C02_40920) hemW 4285235..4286377 (+) 1143 WP_014885195.1 radical SAM family heme chaperone HemW -
  H7R61_RS20845 (WP5S18C02_40930) - 4286441..4286779 (+) 339 WP_023331282.1 endonuclease domain-containing protein -
  H7R61_RS20850 (WP5S18C02_40940) - 4286830..4287546 (-) 717 WP_014885197.1 DUF2884 domain-containing protein -
  H7R61_RS20855 (WP5S18C02_40950) - 4287604..4287930 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35648.71 Da        Isoelectric Point: 6.4113

>NTDB_id=76805 H7R61_RS20815 WP_023337759.1 4282065..4283045(-) (pilT) [Enterobacter cloacae strain WP5-S18-CRE-02]
MDVEEMVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVGALLKAWLNDEQQGSWWAHGQVDFAVTVAEGQRLRG
SAFKQINGVSIALRLLPRTCSPLSLLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
YLYRSERCLIQQREIGQHCPSFVEALRGALREDPDVILLGELRDAETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTPAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSMAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=76805 H7R61_RS20815 WP_023337759.1 4282065..4283045(-) (pilT) [Enterobacter cloacae strain WP5-S18-CRE-02]
ATGGATGTGGAAGAAATGGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGCCGGTCAGGACGCCTTGAACCCGCGCCGTTTCCGCCGCCAGATGTCGGAGCGTTATTAAAAGCGTGGCTCAATG
ATGAGCAGCAGGGAAGCTGGTGGGCTCACGGTCAGGTCGATTTTGCCGTGACGGTGGCAGAAGGCCAGCGTCTGCGCGGG
AGTGCGTTTAAGCAGATTAACGGGGTGTCCATCGCCCTGCGGTTGCTGCCACGCACCTGTTCGCCACTCTCTTTGCTGGG
CGTGCCGCGTGCCATTCCGGAACTGTTATCCAGTGACAACGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGCAAAT
CTACCACTCTGGCGGCGATGGTCGATTTCCTCAACCACCATACTGACGGGCATATCCTCACGCTTGAAGATCCGGTGGAG
TATCTGTACCGGAGCGAACGCTGCCTGATTCAGCAGCGGGAAATAGGTCAGCACTGCCCGTCTTTTGTCGAGGCGCTGCG
CGGAGCGTTACGCGAGGATCCAGATGTGATTTTGCTGGGGGAGCTGCGTGATGCAGAGACGATCCGTCTCGCGTTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACACTGCATACGCGGGGTGCCTCGCAGGCCATTGAGCGGCTGGTGGACACC
TTCCCGGCGCAGGAGAAAGACCCGGTACGTAACCAGCTGGCCGGTAGCCTGCGTGCGGTGCTGGCACAGAGGCTGCTTCC
CGACCTGCAGGGTGGGCGTGTCGCGCTGTATGAACTGCTGGTGAATACCCCGGCGGCGGCAAATCTGATTCGTGAAGGAA
AAACCTGGCAACTGCCCGGTATCATTCAAACAGGCCAGCAGGCGGGAATGCAGAACTTTGATCAAAGCATGGCGGAGAGA
CGGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(73-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2J0PIG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.494
  pilT Vibrio cholerae strain A1552

49.235

100

0.494
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Neisseria meningitidis 8013

46.646

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.383
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383

Multiple sequence alignment