Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   O8I27_RS20515 Genome accession   NZ_CP115000
Coordinates   4270093..4271526 (+) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain CUVET22-793     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4265093..4276526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O8I27_RS20485 (O8I27_20485) - 4265660..4266637 (+) 978 WP_003861852.1 calcium/sodium antiporter -
  O8I27_RS20490 (O8I27_20490) kdsD 4266651..4267637 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  O8I27_RS20495 (O8I27_20495) kdsC 4267652..4268218 (+) 567 WP_063135409.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  O8I27_RS20500 (O8I27_20500) lptC 4268215..4268790 (+) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  O8I27_RS20505 (O8I27_20505) lptA 4268759..4269313 (+) 555 WP_006812136.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  O8I27_RS20510 (O8I27_20510) lptB 4269320..4270045 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  O8I27_RS20515 (O8I27_20515) rpoN/rpoN1 4270093..4271526 (+) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  O8I27_RS20520 (O8I27_20520) hpf 4271549..4271836 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  O8I27_RS20525 (O8I27_20525) ptsN 4271919..4272410 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  O8I27_RS20530 (O8I27_20530) rapZ 4272456..4273310 (+) 855 WP_003861870.1 RNase adapter RapZ -
  O8I27_RS20535 (O8I27_20535) npr 4273307..4273579 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  O8I27_RS20540 (O8I27_20540) mtgA 4273619..4274344 (-) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  O8I27_RS20545 (O8I27_20545) elbB 4274341..4274994 (-) 654 WP_032672055.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=767780 O8I27_RS20515 WP_003861864.1 4270093..4271526(+) (rpoN/rpoN1) [Enterobacter hormaechei strain CUVET22-793]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=767780 O8I27_RS20515 WP_003861864.1 4270093..4271526(+) (rpoN/rpoN1) [Enterobacter hormaechei strain CUVET22-793]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGCGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACCCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACTCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTTCTGAAACGCGTTCAGCGTTTCGACCC
GATAGGTGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACACGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATCCCTGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATATGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGCCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAATATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCTATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48