Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   O8I28_RS20280 Genome accession   NZ_CP114985
Coordinates   4177982..4179415 (+) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain CUVET19-891     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4172982..4184415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O8I28_RS20250 (O8I28_20245) - 4173549..4174526 (+) 978 WP_022651914.1 calcium/sodium antiporter -
  O8I28_RS20255 (O8I28_20250) kdsD 4174540..4175526 (+) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  O8I28_RS20260 (O8I28_20255) kdsC 4175541..4176107 (+) 567 WP_003861856.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  O8I28_RS20265 (O8I28_20260) lptC 4176104..4176679 (+) 576 WP_003861858.1 LPS export ABC transporter periplasmic protein LptC -
  O8I28_RS20270 (O8I28_20265) lptA 4176648..4177202 (+) 555 WP_057059510.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  O8I28_RS20275 (O8I28_20270) lptB 4177209..4177934 (+) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  O8I28_RS20280 (O8I28_20275) rpoN/rpoN1 4177982..4179415 (+) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  O8I28_RS20285 (O8I28_20280) hpf 4179438..4179725 (+) 288 WP_003861866.1 ribosome hibernation promoting factor -
  O8I28_RS20290 (O8I28_20285) ptsN 4179808..4180299 (+) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  O8I28_RS20295 (O8I28_20290) rapZ 4180345..4181199 (+) 855 WP_003861870.1 RNase adapter RapZ -
  O8I28_RS20300 (O8I28_20295) npr 4181196..4181468 (+) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  O8I28_RS20305 (O8I28_20300) mtgA 4181508..4182233 (-) 726 WP_003861874.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  O8I28_RS20310 (O8I28_20305) elbB 4182230..4182883 (-) 654 WP_003861876.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=767714 O8I28_RS20280 WP_003861864.1 4177982..4179415(+) (rpoN/rpoN1) [Enterobacter hormaechei strain CUVET19-891]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=767714 O8I28_RS20280 WP_003861864.1 4177982..4179415(+) (rpoN/rpoN1) [Enterobacter hormaechei strain CUVET19-891]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTCCAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAGACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCACTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAATCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGTGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCTAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAACCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCACTCGATCCGCGTCCGGGGCAGTCGATTCAGACCAG
CGAACCGGAATATGTCATTCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATAGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48