Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   O6P33_RS12300 Genome accession   NZ_CP114976
Coordinates   2640182..2641546 (-) Length   454 a.a.
NCBI ID   WP_269818061.1    Uniprot ID   -
Organism   Denitrificimonas caeni strain CE14     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2635182..2646546
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O6P33_RS12275 (O6P33_12275) sfsA 2636316..2637020 (-) 705 WP_269818057.1 DNA/RNA nuclease SfsA -
  O6P33_RS12280 (O6P33_12280) - 2637010..2638194 (-) 1185 WP_269818058.1 pyridoxal phosphate-dependent aminotransferase -
  O6P33_RS12285 (O6P33_12285) - 2638229..2639035 (-) 807 WP_269819533.1 cobalamin-binding protein -
  O6P33_RS12290 (O6P33_12290) - 2639041..2639460 (-) 420 WP_269818059.1 MFS transporter -
  O6P33_RS12295 (O6P33_12295) ribA 2639457..2640080 (-) 624 WP_269818060.1 GTP cyclohydrolase II -
  O6P33_RS12300 (O6P33_12300) radA 2640182..2641546 (-) 1365 WP_269818061.1 DNA repair protein RadA Machinery gene
  O6P33_RS12305 (O6P33_12305) - 2641705..2641974 (-) 270 WP_269818062.1 hypothetical protein -
  O6P33_RS12310 (O6P33_12310) - 2642133..2642384 (+) 252 WP_269818063.1 hypothetical protein -
  O6P33_RS12315 (O6P33_12315) - 2642381..2643001 (-) 621 WP_269818064.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  O6P33_RS12320 (O6P33_12320) - 2643136..2643675 (+) 540 WP_269818066.1 DUF4136 domain-containing protein -
  O6P33_RS12325 (O6P33_12325) - 2643761..2644213 (+) 453 WP_269818067.1 DMT family transporter -
  O6P33_RS12330 (O6P33_12330) dnaB 2644360..2645754 (-) 1395 WP_269818068.1 replicative DNA helicase -
  O6P33_RS12335 (O6P33_12335) rplI 2645879..2646325 (-) 447 WP_269818069.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49000.57 Da        Isoelectric Point: 7.4050

>NTDB_id=767659 O6P33_RS12300 WP_269818061.1 2640182..2641546(-) (radA) [Denitrificimonas caeni strain CE14]
MAKAKRMYGCTECGATFPKWAGQCGACGTWNTLMETLIEERVAEKSGRAGWAGDQAELKTLAQVSVEKVPRFSTQSSELD
RVLGGGLVDGSVVLLGGDPGIGKSTILLQTLCNMAESISALYVTGEESQQQVAMRARRLELPQDKLKVMTETCIETILAV
ARREKPRVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGSLAGPRVLEHMVDTVLYFEG
ESDGRLRMLRAVKNRFGAVNELGVFGMTDRGLKEVSNPSAIFLNRAQEAVSGSVVMATWEGSRPMLIEVQALVDTSHLGN
PRRVTVGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAIISSLRNRPLEHGLLVFGEIGLSGE
IRPVPSGQERLKEAAKHGFKRAIVPKANAPRETLAGLQVIAVTRLEEALDALFD

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=767659 O6P33_RS12300 WP_269818061.1 2640182..2641546(-) (radA) [Denitrificimonas caeni strain CE14]
ATGGCCAAGGCCAAACGCATGTATGGCTGTACAGAGTGTGGGGCGACTTTTCCAAAGTGGGCAGGACAGTGTGGCGCCTG
TGGTACTTGGAACACTTTAATGGAAACCCTGATTGAAGAGCGCGTTGCCGAAAAAAGCGGTCGCGCCGGCTGGGCTGGTG
ATCAGGCTGAGCTGAAAACCCTTGCGCAAGTCAGCGTTGAAAAGGTGCCACGTTTCTCGACCCAATCCAGCGAGCTTGAT
CGTGTACTTGGCGGTGGTTTAGTAGATGGCTCGGTGGTCTTGCTTGGCGGTGATCCAGGCATTGGTAAGTCCACAATTTT
GCTACAGACGTTGTGCAATATGGCCGAAAGCATTTCAGCTTTATATGTGACCGGTGAAGAGTCGCAGCAGCAGGTGGCCA
TGCGCGCACGGCGGTTAGAGTTACCGCAAGATAAGCTCAAAGTCATGACCGAAACCTGCATCGAAACAATTTTAGCTGTC
GCGCGTCGGGAAAAGCCGCGGGTGATGGTGATTGACTCCATTCAAACCATCTTTACCGAACAGTTGCAGTCCGCTCCCGG
TGGTGTGTCCCAGGTGCGTGAAAGTGCAGCTTTATTGGTGCGTTATGCGAAACAAAGCGGTACTGCGATTTTCTTAGTGG
GCCACGTGACCAAAGAAGGCTCGCTGGCCGGGCCACGGGTATTGGAGCACATGGTTGATACCGTGCTGTATTTTGAAGGT
GAATCCGACGGTCGCTTGCGCATGTTGCGCGCAGTGAAAAACCGTTTTGGTGCAGTCAATGAGCTGGGCGTGTTTGGCAT
GACGGACCGTGGCTTAAAAGAAGTCTCCAACCCATCAGCCATTTTTCTCAATCGCGCCCAAGAGGCAGTGTCCGGCAGTG
TGGTGATGGCCACTTGGGAAGGCTCGCGGCCGATGCTGATTGAGGTGCAGGCCTTAGTGGATACCAGTCACTTGGGCAAT
CCGCGCCGCGTTACTGTGGGCTTGGATCAAAACCGTTTAGCCATGTTGCTGGCTGTATTGCACCGTCACGGTGGTATTCC
CACCCATGATCAAGACGTGTTTTTGAACGTGGTCGGCGGCGTAAAGGTGCTGGAAACGGCATCCGACTTGGCGTTAATGG
CAGCAATTATTTCCAGTTTGCGCAATCGCCCCCTGGAGCACGGGTTGCTGGTGTTTGGTGAAATTGGCTTGTCGGGGGAG
ATTCGCCCAGTACCCAGTGGTCAAGAACGCCTCAAAGAAGCAGCCAAACATGGCTTTAAACGGGCCATTGTGCCAAAAGC
CAATGCACCGCGAGAAACCCTCGCTGGCCTGCAGGTGATTGCGGTGACGCGCCTAGAAGAAGCCCTCGATGCCTTGTTTG
ATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-220)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(350-428)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.458

100

0.485
  radA Streptococcus mitis SK321

46.507

100

0.469
  radA Streptococcus pneumoniae D39

46.916

100

0.469
  radA Streptococcus pneumoniae TIGR4

46.916

100

0.469
  radA Streptococcus pneumoniae R6

46.916

100

0.469
  radA Streptococcus pneumoniae Rx1

46.916

100

0.469
  radA Streptococcus mitis NCTC 12261

46.288

100

0.467