Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   O6U12_RS15315 Genome accession   NZ_CP114899
Coordinates   2873295..2873744 (+) Length   149 a.a.
NCBI ID   WP_124060108.1    Uniprot ID   -
Organism   Bacillus subtilis strain     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2868295..2878744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O6U12_RS15295 (O6U12_15295) hxlR 2869509..2869871 (-) 363 WP_003246265.1 transcriptional activator HxlR -
  O6U12_RS15300 (O6U12_15300) hxlA 2870100..2870732 (+) 633 WP_003234604.1 3-hexulose-6-phosphate synthase -
  O6U12_RS15305 (O6U12_15305) hxlB 2870738..2871295 (+) 558 WP_269767287.1 6-phospho-3-hexuloisomerase -
  O6U12_RS15310 (O6U12_15310) tlpC 2871406..2873127 (+) 1722 WP_095843471.1 methyl-accepting chemotaxis protein TlpC -
  O6U12_RS15315 (O6U12_15315) nucA/comI 2873295..2873744 (+) 450 WP_124060108.1 DNA-entry nuclease Machinery gene
  O6U12_RS15320 (O6U12_15320) nin/comJ 2873771..2874169 (+) 399 WP_021481824.1 DNA-entry nuclease inhibitor Regulator
  O6U12_RS15325 (O6U12_15325) bglC 2874206..2875639 (-) 1434 WP_129133868.1 6-phospho-beta-glucosidase -
  O6U12_RS15330 (O6U12_15330) - 2875711..2876439 (-) 729 WP_021481826.1 AAA family ATPase -
  O6U12_RS15335 (O6U12_15335) - 2876459..2876800 (-) 342 WP_257986415.1 DUF3147 family protein -
  O6U12_RS15340 (O6U12_15340) - 2876788..2877210 (-) 423 WP_257986681.1 MarR family winged helix-turn-helix transcriptional regulator -
  O6U12_RS15345 (O6U12_15345) yckD 2877338..2877670 (-) 333 WP_003246247.1 YckD family protein -
  O6U12_RS15350 (O6U12_15350) yckC 2877752..2878207 (-) 456 WP_137200561.1 RDD family protein -
  O6U12_RS15355 (O6U12_15355) - 2878227..2878424 (-) 198 WP_120363256.1 hypothetical protein -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16456.39 Da        Isoelectric Point: 4.5282

>NTDB_id=767610 O6U12_RS15315 WP_124060108.1 2873295..2873744(+) (nucA/comI) [Bacillus subtilis strain]
MNITTDIIKTILLVIVIIAAAAVGLIKGDFFSADQKTSQTKEYDETISFPSDRYPETAKHIKDAINEGHSEVCTIDRDGA
EERREQSLKDVPSKKGYDRDEWPMAMCKEGGEGASVEYISPADNRGAGSWVGHQLTDYPDGTKVLFTIQ

Nucleotide


Download         Length: 450 bp        

>NTDB_id=767610 O6U12_RS15315 WP_124060108.1 2873295..2873744(+) (nucA/comI) [Bacillus subtilis strain]
GTGAACATCACGACGGACATCATAAAAACGATACTTCTCGTCATCGTCATCATAGCAGCTGCAGCTGTCGGCCTGATAAA
AGGAGACTTTTTCTCAGCTGATCAAAAAACGTCTCAAACGAAAGAATATGATGAAACAATCTCCTTCCCTTCTGACCGGT
ATCCCGAAACGGCCAAGCATATTAAGGATGCGATAAATGAGGGGCACTCAGAGGTGTGCACTATCGACAGAGACGGAGCT
GAGGAACGCCGCGAGCAATCATTAAAGGACGTACCTTCCAAAAAGGGGTATGACAGAGATGAATGGCCAATGGCCATGTG
CAAAGAAGGCGGTGAGGGAGCTTCTGTAGAATACATTTCTCCCGCTGACAACCGCGGAGCAGGCTCTTGGGTCGGGCATC
AGCTTACCGATTACCCAGACGGCACAAAGGTTTTATTCACAATTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(53-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

97.987

100

0.98