Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   F0318_RS03900 Genome accession   NZ_CP126598
Coordinates   792985..793965 (+) Length   326 a.a.
NCBI ID   WP_071999886.1    Uniprot ID   -
Organism   Escherichia coli strain Ec1120     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 787985..798965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F0318_RS03870 (F0318_0003865) yggM 788500..789507 (+) 1008 WP_000745217.1 DUF1202 family protein -
  F0318_RS03875 (F0318_0003870) hemW 789662..790798 (-) 1137 WP_000239928.1 radical SAM family heme chaperone HemW -
  F0318_RS03880 (F0318_0003875) rdgB 790791..791384 (-) 594 WP_001174735.1 XTP/dITP diphosphatase -
  F0318_RS03885 (F0318_0003880) yggU 791392..791682 (-) 291 WP_149488052.1 DUF167 family protein YggU -
  F0318_RS03890 (F0318_0003885) yggT 791679..792245 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  F0318_RS03895 (F0318_0003890) yggS 792263..792967 (-) 705 WP_042004255.1 pyridoxal phosphate homeostasis protein -
  F0318_RS03900 (F0318_0003895) pilT 792985..793965 (+) 981 WP_071999886.1 type IV pilus twitching motility protein PilT Machinery gene
  F0318_RS03905 (F0318_0003900) ruvX 794140..794556 (-) 417 WP_000017111.1 Holliday junction resolvase RuvX -
  F0318_RS03910 (F0318_0003905) yqgE 794556..795119 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  F0318_RS03915 (F0318_0003910) gshB 795228..796178 (-) 951 WP_000593273.1 glutathione synthase -
  F0318_RS03920 (F0318_0003915) rsmE 796191..796922 (-) 732 WP_001300912.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  F0318_RS03925 (F0318_0003920) endA 797002..797709 (-) 708 WP_000286500.1 deoxyribonuclease I -
  F0318_RS03930 (F0318_0003925) yggI 797804..798301 (-) 498 WP_149488053.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35976.14 Da        Isoelectric Point: 5.7980

>NTDB_id=767570 F0318_RS03900 WP_071999886.1 792985..793965(+) (pilT) [Escherichia coli strain Ec1120]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDTPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=767570 F0318_RS03900 WP_071999886.1 792985..793965(+) (pilT) [Escherichia coli strain Ec1120]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATACGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGATTGCGTGGC
AGCGCATTCGCGCAACGGCAAGGTATTTCTCTGGCGTTACGGCTGTTACCTTCGCACTGCCCGCAGCTCGAACAGCTTGG
CGCACCAACGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTTGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGGAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTACTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGA
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.541

100

0.497
  pilT Vibrio cholerae strain A1552

49.541

100

0.497
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.483

100

0.466
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.483

100

0.466
  pilT Pseudomonas aeruginosa PAK

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.954

100

0.451
  pilT Legionella pneumophila strain Lp02

44.954

100

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.824

97.546

0.408
  pilU Vibrio cholerae strain A1552

39.514

100

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.554

100

0.393
  pilB Legionella pneumophila strain ERS1305867

30.89

100

0.362