Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QN223_RS02525 Genome accession   NZ_CP126345
Coordinates   522853..524286 (-) Length   477 a.a.
NCBI ID   WP_109849363.1    Uniprot ID   -
Organism   Escherichia coli strain DX6     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 517853..529286
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QN223_RS02490 (QN223_02490) elbB 518545..519198 (+) 654 WP_001299134.1 isoprenoid biosynthesis glyoxalase ElbB -
  QN223_RS02495 (QN223_02495) mtgA 519195..519923 (+) 729 WP_000047091.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  QN223_RS02500 (QN223_02500) yrbL 519920..520552 (-) 633 WP_000620387.1 PhoP regulatory network protein YrbL -
  QN223_RS02505 (QN223_02505) npr 520765..521037 (-) 273 WP_000216791.1 PTS phosphocarrier protein NPr -
  QN223_RS02510 (QN223_02510) rapZ 521034..521888 (-) 855 WP_000243741.1 RNase adapter RapZ -
  QN223_RS02515 (QN223_02515) ptsN 521934..522425 (-) 492 WP_000183676.1 PTS IIA-like nitrogen regulatory protein PtsN -
  QN223_RS02520 (QN223_02520) hpf 522543..522830 (-) 288 WP_001176599.1 ribosome hibernation promoting factor -
  QN223_RS02525 (QN223_02525) rpoN/rpoN1 522853..524286 (-) 1434 WP_109849363.1 RNA polymerase factor sigma-54 Machinery gene
  QN223_RS02530 (QN223_02530) lptB 524334..525059 (-) 726 WP_000224099.1 LPS export ABC transporter ATP-binding protein -
  QN223_RS02535 (QN223_02535) lptA 525066..525623 (-) 558 WP_000669785.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  QN223_RS02540 (QN223_02540) lptC 525592..526167 (-) 576 WP_000030537.1 LPS export ABC transporter periplasmic protein LptC -
  QN223_RS02545 (QN223_02545) kdsC 526164..526730 (-) 567 WP_000030005.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  QN223_RS02550 (QN223_02550) kdsD 526751..527737 (-) 987 WP_001295557.1 arabinose-5-phosphate isomerase KdsD -
  QN223_RS02555 (QN223_02555) yrbG 527751..528728 (-) 978 WP_000922872.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54092.93 Da        Isoelectric Point: 4.3903

>NTDB_id=767167 QN223_RS02525 WP_109849363.1 522853..524286(-) (rpoN/rpoN1) [Escherichia coli strain DX6]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPE
ELPLDASWDTIYTAGTPSGTSGDYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPRQSIQTGEPEYVIPDVLVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIR
SNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=767167 QN223_RS02525 WP_109849363.1 522853..524286(-) (rpoN/rpoN1) [Escherichia coli strain DX6]
ATGAAGCAAGGTTTGCAACTCAGGCTTAGCCAACAACTGGCGATGACGCCACAGCTCCAACAGGCAATTCGTCTGTTGCA
GTTGTCGACGCTGGAACTTCAGCAGGAGCTACAGCAGGCGCTGGAGAGTAATCCGCTGCTTGAGCAAATCGACACTCATG
AAGAAATCGACACCCGCGAAACGCAAGACAGTGAAACGCTGGACACCGCCGACGCGCTCGAACAAAAAGAGATGCCGGAA
GAGCTGCCGCTCGATGCCAGTTGGGACACCATTTACACCGCTGGTACACCATCCGGCACCAGCGGTGACTACATTGACGA
TGAGCTGCCGGTCTATCAGGGCGAAACGACGCAGACCTTGCAGGATTACCTGATGTGGCAGGTCGAGCTGACACCGTTTT
CCGACACTGACCGCGCTATTGCTACCTCTATCGTCGATGCCGTTGATGACACCGGTTATCTGACTGTCCCGCTGGAAGAT
ATTCTCGAAAGTATGGGCGATGAAGAGATCGACATCGACGAGGTTGAAGCCGTCCTTAAGCGGATCCAACGGTTTGATCC
GGTCGGTGTAGCGGCAAAAGATCTGCGTGACTGCCTGCTAATCCAACTCTCCCAATTCGATAAAACCACGCCGTGGCTGG
AAGAGGCCAGACTGATCATTAGCGATCATCTCGATCTGTTAGCCAATCACGACTTCCGCACTTTAATGCGCGTCACGCGT
CTGAAAGAAGATGTGCTGAAAGAAGCCGTCAATCTGATCCAGTCGCTCGATCCGCGCCCCAGGCAGTCGATCCAGACTGG
CGAACCTGAGTATGTCATTCCAGATGTGCTGGTGCGTAAGCATAACGGTCACTGGACGGTAGAACTCAACAGTGACAGCA
TTCCGCGTCTGCAAATCAACCAGCACTACGCCTCGATGTGCAATAACGCGCGCAACGATGGTGACAGCCAGTTTATCCGC
AGCAATCTGCAGGATGCCAAATGGTTGATCAAGAGTCTGGAAAGCCGTAACGATACGCTACTGCGCGTGAGTCGCTGTAT
CGTTGAACAGCAGCAAGCCTTCTTTGAGCAAGGTGAAGAATATATGAAACCGATGGTACTGGCCGATATCGCCCAGGCTG
TCGAAATGCATGAATCGACGATATCTCGCGTGACCACGCAAAAATACCTGCATAGTCCACGAGGCATTTTTGAACTGAAG
TATTTCTTTTCCAGTCACGTCAATACCGAGGGCGGCGGCGAAGCTTCCTCCACGGCGATTCGTGCGCTGGTGAAGAAATT
AATCGCGGCGGAAAACCCAGCGAAACCGTTGAGCGACAGCAAGTTAACCTCTTTGCTGTCGGAACAAGGTATCATGGTGG
CACGCCGCACTGTTGCGAAGTACCGAGAGTCTTTATCCATTCCGCCGTCAAACCAGCGTAAACAGCTCGTTTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

44.024

100

0.463