Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   O3276_RS22250 Genome accession   NZ_CP114771
Coordinates   5318721..5320763 (+) Length   680 a.a.
NCBI ID   WP_269673267.1    Uniprot ID   -
Organism   Endozoicomonas sp. GU-1 strain Ap1-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5313721..5325763
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O3276_RS22230 (O3276_22230) comM 5315698..5316762 (+) 1065 WP_101745016.1 pilus assembly protein PilM Machinery gene
  O3276_RS22235 (O3276_22235) - 5316762..5317325 (+) 564 WP_269673264.1 PilN domain-containing protein -
  O3276_RS22240 (O3276_22240) - 5317322..5317933 (+) 612 WP_269673265.1 type 4a pilus biogenesis protein PilO -
  O3276_RS22245 (O3276_22245) - 5317930..5318466 (+) 537 WP_269673266.1 pilus assembly protein PilP -
  O3276_RS22250 (O3276_22250) pilQ 5318721..5320763 (+) 2043 WP_269673267.1 type IV pilus secretin PilQ family protein Machinery gene
  O3276_RS22255 (O3276_22255) aroK 5321054..5321575 (+) 522 WP_101745020.1 shikimate kinase AroK -
  O3276_RS22260 (O3276_22260) aroB 5321590..5322671 (+) 1082 Protein_4366 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 680 a.a.        Molecular weight: 73960.92 Da        Isoelectric Point: 4.5107

>NTDB_id=767017 O3276_RS22250 WP_269673267.1 5318721..5320763(+) (pilQ) [Endozoicomonas sp. GU-1 strain Ap1-2]
MNISPLLQARAVRGLLYTLLAMLPISAWGVTLKNIDTAALPGEMVEIRLSFDGAAPVAHGYSIEKPPRISIDLPFTRSEL
PKYNEIGFNNAKSVTVLEAGDRTRLVINLQQPTRFSTQADGRTLSIYLGENNSTTAVQRELPQRNQSSYQAAEMRRGITD
IDFQRGDDGEGEVIIALSDARIPMDMNEQGGRIRLEFQGNVLPQSLRNRLDVIDFATPIKFIDAAVEDGNSIITIEPKGE
FEYLAYQTDNLLTVSVKAPDPKDRSRRKAGLSYKGDKLSLNFQDIEVRAVLQLIADFTDLNLVASDTVGGNVTLRLQNVP
WDQALDIVLKAKGLDKRLEGNVLTVAPAAEIAAREREQLENEKQIRELAPVYTDLVQINYADAEEIATVLKGAEGASLLT
ERGSVQVVARTNSLLIKDTQAKLDELRALIDQLDIPIRQVMIEARIVTLSSNFQEELGVRWSGSKNDVLKGDNRDLSIGG
GQDDDVFTDLSVTGGASSAIAIGFSTNGGTILNLELSALLSDGGGEVISQPKVITADKKTAIIKSGKEIPYEEKTSSGAT
SVAFKDAVLGLEVTPQITPDGRVIMDIKINNDDTTDQASNNVPIISTNEITTQVLVEDGETVVLGGVFKQSKKQGVIKVP
VLGDIPLVGGLFRNKTESDDKEELLVFITPRIITEGVSLR

Nucleotide


Download         Length: 2043 bp        

>NTDB_id=767017 O3276_RS22250 WP_269673267.1 5318721..5320763(+) (pilQ) [Endozoicomonas sp. GU-1 strain Ap1-2]
ATGAATATTAGTCCCCTGTTGCAGGCCAGGGCTGTCCGAGGGCTTCTGTATACGTTGCTGGCCATGTTGCCTATTTCCGC
GTGGGGTGTCACCCTGAAAAACATCGATACAGCGGCATTGCCGGGTGAGATGGTTGAGATACGTTTGAGCTTTGATGGCG
CGGCACCGGTTGCTCATGGCTACAGCATTGAGAAGCCTCCCCGGATTTCCATAGACCTGCCTTTTACCCGCAGTGAACTC
CCCAAGTACAACGAGATTGGTTTTAATAATGCCAAAAGTGTGACTGTGCTTGAGGCTGGAGACAGAACCCGCCTGGTTAT
TAATCTTCAGCAGCCTACCCGCTTCAGCACCCAGGCTGATGGCAGGACGCTGAGCATTTACCTTGGCGAGAATAACAGCA
CCACGGCGGTGCAAAGGGAGTTACCTCAGCGGAATCAGTCATCCTATCAAGCTGCAGAAATGCGAAGAGGTATTACCGAT
ATTGATTTCCAGCGAGGTGATGATGGTGAGGGCGAGGTTATCATCGCCCTGTCCGATGCCAGGATTCCGATGGATATGAA
TGAGCAGGGTGGGCGTATCCGTCTGGAGTTTCAGGGCAATGTATTGCCACAGAGCCTGAGAAATCGCCTGGATGTTATCG
ACTTTGCTACACCGATCAAGTTTATTGATGCCGCTGTTGAGGATGGTAACAGCATTATTACCATAGAGCCCAAGGGAGAG
TTTGAGTATCTGGCCTATCAGACAGACAACTTGCTGACTGTCAGTGTCAAGGCTCCAGACCCTAAAGATCGCAGTAGACG
AAAAGCAGGGCTCTCCTATAAAGGAGATAAGCTGTCGTTAAACTTTCAGGATATTGAAGTCCGGGCGGTGCTTCAGTTGA
TCGCTGATTTTACCGATCTGAATCTGGTGGCATCGGATACGGTTGGGGGGAATGTAACCCTGCGACTGCAAAATGTTCCC
TGGGATCAGGCGCTTGATATTGTGCTTAAGGCTAAAGGGCTCGATAAGCGTTTGGAAGGCAATGTTCTTACCGTGGCACC
GGCAGCGGAAATTGCTGCACGTGAACGTGAGCAGCTGGAGAACGAAAAACAGATTCGTGAGTTAGCCCCGGTATATACCG
ATCTGGTTCAGATTAATTACGCCGACGCTGAAGAAATTGCCACGGTACTGAAAGGCGCAGAAGGTGCCAGCCTGCTGACA
GAGAGGGGCTCGGTTCAGGTGGTGGCAAGAACCAATAGTCTGTTGATCAAGGATACGCAGGCCAAGCTGGATGAATTAAG
AGCTTTGATCGATCAGCTGGATATTCCAATTCGGCAGGTGATGATTGAGGCTCGTATCGTGACACTCAGCTCCAACTTCC
AGGAAGAACTGGGTGTTAGGTGGAGTGGGTCAAAAAATGATGTGCTCAAGGGCGATAACAGGGATCTCTCGATTGGTGGT
GGGCAGGATGATGATGTATTTACTGACCTGAGTGTCACTGGAGGGGCTTCAAGTGCTATTGCTATCGGCTTCTCCACCAA
TGGTGGCACGATTTTGAACCTTGAGCTGTCTGCGCTGTTGAGTGATGGCGGCGGTGAAGTCATTTCACAGCCAAAAGTTA
TTACCGCCGATAAGAAAACGGCGATAATCAAATCAGGTAAAGAAATTCCGTACGAAGAGAAAACCTCCAGTGGGGCCACT
TCTGTTGCGTTCAAGGATGCGGTGCTGGGGCTGGAAGTGACACCTCAGATTACCCCGGATGGTCGAGTTATTATGGATAT
CAAGATCAATAATGACGATACCACTGATCAGGCATCCAATAACGTTCCAATTATTTCTACCAATGAGATTACTACCCAGG
TTCTGGTTGAAGATGGTGAAACAGTCGTTCTTGGTGGTGTCTTTAAGCAGAGTAAAAAACAAGGTGTTATCAAAGTACCA
GTCCTGGGTGATATTCCCCTGGTCGGTGGTTTGTTCCGCAATAAAACAGAGAGTGATGATAAAGAAGAACTTCTGGTCTT
TATAACGCCCAGAATTATTACTGAAGGTGTTTCATTGCGTTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(375-439)

Bacterial type II and III secretion system protein (Secretin)

(519-673)

Secretin and TonB N terminus short domain (STN)

(301-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

49.498

100

0.507
  pilQ Acinetobacter baumannii D1279779

40.449

100

0.424
  pilQ Acinetobacter baumannii strain A118

40.449

100

0.424
  comQ Acinetobacter baylyi ADP1

40.171

100

0.415
  pilQ Legionella pneumophila strain Lp02

40.33

98.088

0.396
  pilQ Legionella pneumophila strain ERS1305867

40.03

98.088

0.393