Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O3276_RS18635 Genome accession   NZ_CP114771
Coordinates   4508822..4510534 (-) Length   570 a.a.
NCBI ID   WP_269676017.1    Uniprot ID   -
Organism   Endozoicomonas sp. GU-1 strain Ap1-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4503822..4515534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O3276_RS18625 (O3276_18625) - 4503827..4505149 (-) 1323 WP_269672660.1 hypothetical protein -
  O3276_RS18630 (O3276_18630) putA 4505553..4508732 (+) 3180 WP_269676016.1 bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA -
  O3276_RS18635 (O3276_18635) pilB 4508822..4510534 (-) 1713 WP_269676017.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O3276_RS18640 (O3276_18640) - 4510806..4512758 (-) 1953 WP_332328135.1 DUF1302 family protein -
  O3276_RS18645 (O3276_18645) - 4513159..4514514 (-) 1356 WP_269672662.1 ankyrin repeat domain-containing protein -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 62301.15 Da        Isoelectric Point: 4.9632

>NTDB_id=767012 O3276_RS18635 WP_269676017.1 4508822..4510534(-) (pilB) [Endozoicomonas sp. GU-1 strain Ap1-2]
MANKPLTGLAARLLADQLMEEADIHNAEAAAAKANKPLARYLLEKKLVNGNALTLATAEEFGLPVIDLRAFDKEHFVKDV
VEEKLMRKHNVLPLIKRGSHLFAAVSDPSNLQGLDEIRFNTGLTIHTVLSEPEILEKMIDTALDDTGGMFADDDGMVGLE
DLEVSAVDDSGDDALGTSGGDGEDDTPVVKFVNKMLLSAIKGGASDLHFEPYERTYRVRFRTDGVLHEVNRPPVSLGSRI
ASRLKIMSAMDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVLRILDPSSAKMGIDALGYEEEQKEMYMTALH
QPQGMILVTGPTGSGKTVSLYTGLNILNTNERNISTAEDPVEINLEGINQVNVNPKQGMDFSQALRAFLRQDPDIIMVGE
IRDLETANIAIKAAQTGHMVMSTLHTNSAAETLTRLQNMGVPSFNIATSVSLIIAQRLARRLCSQCKVKVDIPKETLLEE
GFTEAQADSAEIFGPKGCDNCKGGYKGRVGIYEVVSITPGMQQIIMAEGNSLEISEQAQKEGFNNLRQSGLLKALQGVTS
LEEVNRVTQD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=767012 O3276_RS18635 WP_269676017.1 4508822..4510534(-) (pilB) [Endozoicomonas sp. GU-1 strain Ap1-2]
ATGGCAAACAAACCTCTGACCGGGCTGGCGGCCCGCCTGCTGGCCGATCAATTGATGGAAGAGGCCGACATTCACAACGC
TGAAGCCGCTGCTGCCAAGGCCAACAAACCGCTCGCCCGATATCTGCTGGAAAAAAAGCTGGTCAACGGTAATGCCCTGA
CGCTCGCCACTGCCGAAGAGTTTGGCCTGCCAGTGATCGATCTCAGAGCCTTTGACAAAGAGCATTTTGTCAAAGACGTC
GTCGAAGAAAAACTGATGCGGAAGCACAATGTGCTGCCGCTGATCAAGCGGGGCTCACACCTGTTTGCCGCCGTTTCTGA
TCCGTCCAACCTTCAGGGCCTGGATGAAATCCGCTTTAATACCGGCCTGACCATTCATACGGTCCTGTCAGAGCCCGAAA
TTCTGGAGAAGATGATTGATACTGCCCTGGATGATACCGGTGGCATGTTTGCCGACGACGATGGGATGGTCGGTCTTGAA
GATCTTGAAGTCAGTGCGGTTGATGATTCAGGAGATGATGCCCTGGGAACCTCCGGGGGCGACGGCGAGGATGACACGCC
GGTAGTGAAGTTCGTCAATAAAATGCTGCTGTCCGCCATCAAAGGCGGTGCGTCGGATCTGCACTTTGAGCCCTATGAAA
GAACCTATCGGGTTCGTTTCAGAACCGACGGTGTGCTCCATGAAGTCAATCGCCCCCCGGTCAGCCTCGGGTCCAGGATT
GCTTCACGACTGAAGATCATGTCGGCCATGGATATTTCCGAGCGCAGAAAGCCTCAGGATGGCCGGATAAAAATGAAGCT
GTCGAAAAGCAAGGCCATCGACTTTCGGGTCAACACGCTGCCGACGCTCTGGGGTGAGAAAATCGTACTTCGTATCCTCG
ATCCTTCCAGCGCCAAAATGGGGATTGATGCTCTGGGCTACGAAGAAGAGCAGAAAGAGATGTATATGACCGCCCTGCAC
CAGCCACAGGGCATGATTCTGGTTACCGGACCCACCGGTTCGGGAAAAACGGTATCGCTCTATACCGGTTTGAATATTCT
CAATACCAATGAGCGCAACATTTCCACCGCGGAGGACCCGGTGGAAATTAACCTGGAAGGTATTAACCAGGTTAACGTCA
ATCCCAAACAGGGTATGGACTTCAGCCAGGCGTTGCGGGCTTTCCTGCGTCAGGATCCGGATATCATCATGGTGGGTGAG
ATACGGGACCTGGAAACCGCCAACATTGCCATCAAGGCGGCCCAGACCGGCCATATGGTGATGTCCACCCTGCACACCAA
CAGCGCGGCAGAGACCCTGACCCGTTTGCAGAACATGGGGGTGCCATCATTTAACATCGCAACCTCGGTCAGCCTGATTA
TTGCCCAGCGTCTGGCACGAAGACTGTGCAGTCAGTGCAAGGTAAAAGTTGACATCCCGAAAGAAACACTGCTGGAAGAG
GGCTTTACCGAAGCGCAGGCGGACTCCGCCGAGATTTTTGGCCCTAAAGGCTGTGACAACTGCAAGGGCGGCTATAAAGG
TCGTGTGGGTATTTATGAGGTGGTGTCGATCACCCCCGGCATGCAGCAAATCATTATGGCCGAAGGTAACTCCCTGGAAA
TCTCGGAACAGGCCCAGAAAGAAGGCTTTAACAACCTTCGCCAATCGGGGCTATTAAAGGCGCTGCAAGGGGTTACCAGC
CTTGAAGAGGTGAACCGGGTTACACAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-145)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.035

100

0.604
  pilB Acinetobacter baylyi ADP1

57.319

99.474

0.57
  pilB Legionella pneumophila strain ERS1305867

54.386

100

0.544
  pilB Vibrio cholerae strain A1552

49.644

98.596

0.489
  pilF Neisseria gonorrhoeae MS11

47.719

100

0.477
  pilB Vibrio campbellii strain DS40M4

50

92.632

0.463
  pilB Vibrio parahaemolyticus RIMD 2210633

51.468

89.649

0.461
  pilB/pilB1 Synechocystis sp. PCC 6803

39.885

91.93

0.367