Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O4M85_RS25800 Genome accession   NZ_CP114761
Coordinates   5508804..5510504 (+) Length   566 a.a.
NCBI ID   WP_003120945.1    Uniprot ID   A0A1L7HA63
Organism   Pseudomonas aeruginosa strain NF143349     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5503804..5515504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4M85_RS25775 (O4M85_25775) ampD 5503841..5504407 (-) 567 WP_009875882.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  O4M85_RS25780 (O4M85_25780) - 5504558..5506813 (-) 2256 WP_019726810.1 DUF1631 domain-containing protein -
  O4M85_RS25785 (O4M85_25785) nadC 5507099..5507947 (+) 849 WP_003116246.1 carboxylating nicotinate-nucleotide diphosphorylase -
  O4M85_RS25795 (O4M85_25795) - 5508100..5508573 (-) 474 WP_015503553.1 pilin -
  O4M85_RS25800 (O4M85_25800) pilB 5508804..5510504 (+) 1701 WP_003120945.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O4M85_RS25805 (O4M85_25805) pilC 5510508..5511725 (+) 1218 WP_010792214.1 type II secretion system F family protein Machinery gene
  O4M85_RS25810 (O4M85_25810) pilD 5511728..5512600 (+) 873 WP_019726811.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  O4M85_RS25815 (O4M85_25815) coaE 5512597..5513208 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  O4M85_RS25820 (O4M85_25820) yacG 5513205..5513405 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  O4M85_RS25825 (O4M85_25825) - 5513442..5513651 (-) 210 WP_003120946.1 hypothetical protein -
  O4M85_RS25830 (O4M85_25830) - 5513757..5514446 (-) 690 WP_003103868.1 energy-coupling factor ABC transporter permease -
  O4M85_RS25835 (O4M85_25835) - 5514443..5514913 (-) 471 WP_019486073.1 hypothetical protein -
  O4M85_RS25840 (O4M85_25840) - 5514910..5515335 (-) 426 WP_003103865.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62385.25 Da        Isoelectric Point: 5.7055

>NTDB_id=766955 O4M85_RS25800 WP_003120945.1 5508804..5510504(+) (pilB) [Pseudomonas aeruginosa strain NF143349]
MNDSIQLSGLSRQLVQANLLDEKTAVQAQAQAQRNKLSLVTHLVQNKLVSGLALAELSAEQFGIAYCDLNSLDKESFPRD
AISEKLVRQHRVIPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGLAIDKLFESATDGLAGLDDVDLE
GLDIGSAEKSTQEDTSAEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKSKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPRETLLHEGFPE
DKIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=766955 O4M85_RS25800 WP_003120945.1 5508804..5510504(+) (pilB) [Pseudomonas aeruginosa strain NF143349]
ATGAACGACAGCATCCAACTGAGCGGCCTGTCCCGACAGCTCGTCCAAGCCAATCTACTCGACGAAAAGACCGCCGTGCA
GGCCCAGGCGCAGGCGCAGCGCAACAAGCTGTCGCTGGTGACGCACCTGGTGCAGAACAAGCTGGTAAGCGGCCTGGCCC
TGGCCGAATTATCCGCCGAGCAGTTCGGTATCGCCTACTGCGACCTGAATAGCCTGGACAAGGAAAGCTTTCCGCGGGAC
GCCATCAGCGAAAAGCTGGTCCGCCAGCACCGGGTCATCCCTTTGTGGCGGCGCGGCAACAAGCTCTTCGTAGGCATCTC
CGATCCCGCCAACCACCAGGCCATCAATGATGTCCAGTTCAGCACCGGCCTGACGACCGAAGCGATTCTGGTCGAAGACG
ACAAGCTTGGCCTGGCCATCGACAAACTGTTCGAAAGCGCTACCGATGGTCTCGCAGGCCTCGACGATGTTGACCTCGAG
GGACTGGATATCGGTAGCGCGGAAAAGTCCACCCAGGAGGATACCAGCGCAGAAGCGGACGACGCTCCTGTAGTACGTTT
CGTCAACAAGATGCTGCTGGATGCCATAAAGGGTGGCTCTTCCGACCTGCACTTCGAGCCCTACGAGAAGATCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAAGTGGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGACATTTCCGAACGGCGCAAACCGCAGGACGGACGGATCAAGATGCGCGTGTCGAAGAGCAA
GTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CACAAATGGGCATCGACGCCCTCGGCTACGAGGAGGACCAGAAGGAACTCTACCTGGCCGCACTCAAGCAGCCACAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTATCGCTATACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATTTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGACATATGGTGATGTCCACCCTACACACCAACAGCGCCGCCGA
GACCCTGACTCGCCTGCTGAACATGGGCGTGCCGGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGAGGTGCCGAGGGAAACCCTGCTTCACGAGGGCTTTCCGGAA
GACAAGATCGGTACCTTCAAGCTTTATTCGCCGGTGGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGGTAT
TTATGAAGTAGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L7HA63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.692

100

0.583
  pilB Acinetobacter baylyi ADP1

57.371

99.47

0.571
  pilB Legionella pneumophila strain ERS1305867

55.124

100

0.551
  pilB Vibrio parahaemolyticus RIMD 2210633

51.064

99.647

0.509
  pilF Neisseria gonorrhoeae MS11

50.712

99.293

0.504
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilB Vibrio campbellii strain DS40M4

49.823

99.647

0.496
  pilF Thermus thermophilus HB27

39.572

99.117

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.607

93.11

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

38.563

93.463

0.36