Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   O4M77_RS02285 Genome accession   NZ_CP114606
Coordinates   492758..493558 (+) Length   266 a.a.
NCBI ID   WP_004782101.1    Uniprot ID   A0AAU0NFS0
Organism   Acinetobacter sp. YWS30-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 487758..498558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4M77_RS02260 (O4M77_02265) ilvC 487847..488863 (+) 1017 WP_005232140.1 ketol-acid reductoisomerase -
  O4M77_RS02265 (O4M77_02270) - 490004..490555 (+) 552 WP_004782094.1 phosphatase PAP2 family protein -
  O4M77_RS02270 (O4M77_02275) iscX 490644..490844 (+) 201 WP_005232138.1 Fe-S cluster assembly protein IscX -
  O4M77_RS02275 (O4M77_02280) ndk 490955..491386 (+) 432 WP_004782097.1 nucleoside-diphosphate kinase -
  O4M77_RS02280 (O4M77_02285) rlmN 491508..492743 (+) 1236 WP_005232136.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  O4M77_RS02285 (O4M77_02290) pilF 492758..493558 (+) 801 WP_004782101.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  O4M77_RS02290 (O4M77_02295) - 493549..494334 (+) 786 WP_323713754.1 RodZ domain-containing protein -
  O4M77_RS02295 (O4M77_02300) ispG 494358..495479 (+) 1122 WP_004782104.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  O4M77_RS02300 (O4M77_02305) hisS 495483..496778 (+) 1296 WP_125279599.1 histidine--tRNA ligase -
  O4M77_RS02305 (O4M77_02310) - 496809..497507 (+) 699 WP_323713755.1 YfgM family protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29962.06 Da        Isoelectric Point: 9.0592

>NTDB_id=766825 O4M77_RS02285 WP_004782101.1 492758..493558(+) (pilF) [Acinetobacter sp. YWS30-1]
MSNLTSKLALVSVVCGSLVLTACQTPDLGQKDPEKATKVRTQLAVEYLKNGDLDSAKRALDQALETNPRDSQANMMMGVL
LQQEGSKPNLEKAEGYFKRAISADPKNAQARNNYGTYLYQLERYKDAIEQFEIAGATLGYDQRFRALENMGRIYLKQGDL
ANAEKSFKQALQANRASYISMLELAEIFYLNQQFPAATQMYEQFVRGVGQKNQSARALWIGIRTARASGDQLGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=766825 O4M77_RS02285 WP_004782101.1 492758..493558(+) (pilF) [Acinetobacter sp. YWS30-1]
TTGAGTAATCTGACATCTAAACTTGCTTTGGTTTCAGTAGTATGTGGGTCTTTGGTTTTAACTGCGTGTCAAACGCCAGA
TCTGGGTCAGAAGGACCCGGAAAAAGCCACCAAGGTTCGTACTCAGTTGGCGGTTGAATATCTTAAAAACGGTGATCTGG
ATTCAGCTAAACGTGCGCTGGATCAGGCACTGGAAACTAATCCGCGTGATTCGCAAGCCAATATGATGATGGGCGTGCTG
CTTCAGCAGGAAGGCAGCAAACCTAATCTTGAAAAGGCCGAAGGTTATTTCAAGCGTGCAATTTCGGCAGATCCAAAAAA
TGCCCAGGCACGAAATAACTACGGCACCTATTTATATCAGCTTGAGCGCTATAAAGATGCGATCGAGCAGTTCGAAATTG
CCGGCGCAACTTTAGGTTATGACCAGCGATTTCGTGCCCTTGAAAATATGGGACGGATTTACCTGAAACAGGGTGATTTG
GCGAATGCTGAAAAATCATTCAAACAGGCACTACAGGCTAATCGCGCTTCGTATATATCAATGTTAGAGTTGGCAGAAAT
TTTTTATCTGAACCAGCAGTTCCCGGCCGCGACTCAAATGTATGAACAGTTTGTACGTGGCGTGGGGCAGAAAAACCAGA
GTGCCCGTGCGCTCTGGATTGGCATTCGCACTGCGCGTGCCAGTGGCGATCAGTTAGGCATGCAGGTTTTGGTCAATCAA
TTACGTGCATTGTTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(108-175)

Tetratricopeptide repeat (TPR_14)

(40-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.068

100

0.771
  pilF Acinetobacter baylyi ADP1

70.037

100

0.703