Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   O4J62_RS10475 Genome accession   NZ_CP114601
Coordinates   2178489..2179277 (-) Length   262 a.a.
NCBI ID   WP_371971077.1    Uniprot ID   -
Organism   Acinetobacter towneri strain TT1-3_T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2173489..2184277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4J62_RS10455 (O4J62_10455) - 2174514..2175215 (-) 702 WP_004975103.1 YfgM family protein -
  O4J62_RS10460 (O4J62_10460) hisS 2175246..2176541 (-) 1296 WP_323712469.1 histidine--tRNA ligase -
  O4J62_RS10465 (O4J62_10465) ispG 2176545..2177666 (-) 1122 WP_004975100.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  O4J62_RS10470 (O4J62_10470) - 2177689..2178498 (-) 810 WP_004975098.1 helix-turn-helix domain-containing protein -
  O4J62_RS10475 (O4J62_10475) pilF 2178489..2179277 (-) 789 WP_371971077.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  O4J62_RS10480 (O4J62_10480) rlmN 2179353..2180585 (-) 1233 WP_004975094.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  O4J62_RS10485 (O4J62_10485) ndk 2180722..2181153 (-) 432 WP_004975092.1 nucleoside-diphosphate kinase -
  O4J62_RS10490 (O4J62_10490) iscX 2181315..2181515 (-) 201 WP_004975089.1 Fe-S cluster assembly protein IscX -
  O4J62_RS10495 (O4J62_10495) - 2181617..2182168 (-) 552 WP_004975087.1 phosphatase PAP2 family protein -
  O4J62_RS10500 (O4J62_10500) - 2182194..2183477 (-) 1284 WP_323712470.1 glycosyltransferase family 1 protein -
  O4J62_RS10505 (O4J62_10505) - 2183739..2184164 (-) 426 WP_179990495.1 hypothetical protein -

Sequence

Protein


Download         Length: 262 a.a.        Molecular weight: 29451.33 Da        Isoelectric Point: 8.7703

>NTDB_id=766807 O4J62_RS10475 WP_371971077.1 2178489..2179277(-) (pilF) [Acinetobacter towneri strain TT1-3_T]
MAQITTVCISAIFAAACQTTAPTLGQKDPEKAVQVRTQLAAEYLKNGDLDSAKRALDQALESDSRDSTANMMMGVLLQQE
GSKLNLEKADAYFKRAIAADPKNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDIENAE
KSFKQALQANRDSYVSMLELSEILYLRQQFAPARQLYEQFVRAVGQKNQGARALWIGIRLARANGDQLGTQVLVNQLRAL
FPNSQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 789 bp        

>NTDB_id=766807 O4J62_RS10475 WP_371971077.1 2178489..2179277(-) (pilF) [Acinetobacter towneri strain TT1-3_T]
ATCGCACAAATTACAACTGTTTGTATTTCGGCAATTTTTGCTGCAGCTTGTCAAACCACAGCTCCAACCTTAGGGCAAAA
AGACCCTGAAAAAGCGGTGCAAGTACGTACGCAGTTGGCAGCAGAATACTTAAAAAATGGCGATTTAGACTCAGCCAAAC
GCGCACTTGATCAAGCTTTGGAAAGCGATTCACGTGATTCCACCGCTAATATGATGATGGGCGTGTTGCTGCAACAAGAA
GGCAGCAAGCTGAATTTAGAAAAAGCCGATGCGTATTTTAAGCGTGCCATTGCCGCTGACCCAAAAAATGCACAAGCGCG
TAACAACTATGGTACGTATTTATATCAAGTAGAACGCTATAATGATGCGATTGAGCAGCTCAGTATTGCAGGTGCAACAC
TGGGTTATGATCAACGCTTTAGAGCATTGGAAAATGTAGGTCGTATTTACTTGAAATTAGGCGATATAGAGAATGCCGAA
AAGTCATTCAAACAAGCCTTGCAAGCCAATCGCGATTCTTATGTTTCAATGTTAGAGTTGTCGGAAATTTTATATTTAAG
ACAACAATTTGCACCCGCGCGACAATTGTATGAACAATTTGTACGTGCTGTGGGGCAAAAAAATCAGGGTGCGCGTGCCT
TATGGATTGGGATTCGTTTAGCACGTGCCAATGGCGATCAATTGGGAACTCAGGTTTTAGTGAACCAGCTGCGTGCATTA
TTTCCCAATAGCCAAGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(93-125)

Tetratricopeptide repeat (TPR_16)

(39-76)

Tetratricopeptide repeat (TPR_16)

(147-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.778

96.183

0.748
  pilF Acinetobacter baylyi ADP1

72.047

96.947

0.698