Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   O4546_RS01200 Genome accession   NZ_CP114590
Coordinates   255425..257170 (+) Length   581 a.a.
NCBI ID   WP_269587305.1    Uniprot ID   -
Organism   Salinivibrio kushneri strain TGB19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 250425..262170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4546_RS01180 (O4546_01180) - 252861..253724 (+) 864 WP_269587301.1 hypothetical protein -
  O4546_RS01185 (O4546_01185) - 253721..254302 (+) 582 WP_269587302.1 PilN domain-containing protein -
  O4546_RS01190 (O4546_01190) - 254299..254880 (+) 582 WP_269587303.1 type 4a pilus biogenesis protein PilO -
  O4546_RS01195 (O4546_01195) - 254901..255428 (+) 528 WP_269587304.1 pilus assembly protein PilP -
  O4546_RS01200 (O4546_01200) pilQ 255425..257170 (+) 1746 WP_269587305.1 type IV pilus secretin PilQ Machinery gene
  O4546_RS01205 (O4546_01205) aroK 257315..257833 (+) 519 WP_025739049.1 shikimate kinase AroK -
  O4546_RS01210 (O4546_01210) aroB 257853..258932 (+) 1080 WP_069361857.1 3-dehydroquinate synthase -
  O4546_RS01215 (O4546_01215) - 258944..260569 (+) 1626 WP_269587306.1 AAA family ATPase -
  O4546_RS01220 (O4546_01220) dam 260642..261472 (+) 831 WP_269587307.1 adenine-specific DNA-methyltransferase -
  O4546_RS01225 (O4546_01225) - 261494..261682 (-) 189 WP_069361854.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 62744.24 Da        Isoelectric Point: 5.2652

>NTDB_id=766675 O4546_RS01200 WP_269587305.1 255425..257170(+) (pilQ) [Salinivibrio kushneri strain TGB19]
MKASRVKRTLGSGLAQAVIVTVFVCGMVWSTVVGAAEAVTLVQAQLTSPTPKETQISLRFDSASVMADFRQLSPKQLMIR
LPQVVVKPDLLGRMKGQETGLPVNAVHITQVAGYTKIEIDAQQAMTFDYQQQHQQLIITLRDADPATAQAPDPDAPISIN
FQDIPVRAVLQLIAEQNQFNLVTTDSVQGNITMRIDGVPWQDVLDVILRVKGLDKRQRGDVMLVAPRKELMAQEKQDLEM
AQQADALLPLSSAVITVNYAKAADVAALIRGQGQGVSLLSPRGAVSLDSRTNAIIIRDIEKNIDRIRTLVSHLDVPVKQV
EIEARIVTVEQGSVDELGVRWGLLNRNGSTTVGPSVEGNLMWDSNVTGQGPESLNEAATDGVNMGDFLNVNLAAASPSAS
SMAMQVAKLGNDLLLDLELSALESERKAEVVSSPRLITTNKKAAYIEQGTELPYLEASSSGAVAVSFKKAVLSLSVTPQI
TPNNTLVLDLKLTQDMPEKAVVAGKGEAMSISTKRINTQVLAENGETVVLGGIFQQTMINKETQVPLLGDIPLVGNLFKH
QLKDHAKRELLIFVTPKIVTD

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=766675 O4546_RS01200 WP_269587305.1 255425..257170(+) (pilQ) [Salinivibrio kushneri strain TGB19]
ATGAAAGCAAGCAGGGTAAAACGCACACTCGGATCAGGACTGGCGCAAGCTGTCATTGTGACTGTTTTCGTTTGTGGGAT
GGTATGGAGTACCGTTGTGGGGGCCGCTGAGGCAGTGACCTTAGTGCAGGCGCAACTTACGTCTCCCACGCCGAAAGAGA
CGCAGATCAGCTTACGTTTTGACAGTGCCTCGGTGATGGCGGACTTTCGCCAGTTGAGCCCTAAGCAGCTTATGATTCGA
TTACCTCAGGTGGTCGTCAAGCCAGACTTACTTGGGCGGATGAAAGGGCAAGAGACGGGGTTGCCTGTGAACGCCGTTCA
CATCACCCAGGTCGCAGGCTACACCAAGATAGAAATTGATGCCCAGCAAGCGATGACGTTTGATTACCAACAACAGCATC
AGCAATTAATTATCACCTTGCGCGACGCCGATCCGGCGACCGCCCAAGCCCCTGATCCCGATGCACCGATTTCCATCAAT
TTCCAAGATATTCCTGTGCGGGCGGTGTTACAACTGATTGCCGAGCAAAATCAATTTAATTTGGTGACCACTGACAGTGT
GCAGGGCAATATCACCATGCGTATCGACGGGGTACCCTGGCAAGATGTGCTGGATGTGATTCTCAGGGTCAAGGGGTTAG
ACAAGCGCCAACGTGGGGATGTGATGTTAGTGGCCCCTCGTAAAGAACTGATGGCGCAAGAAAAACAAGATCTCGAGATG
GCGCAACAAGCAGACGCGCTGTTGCCTTTGTCTTCTGCCGTGATCACCGTTAACTACGCCAAAGCCGCTGACGTCGCGGC
GTTAATCCGAGGCCAGGGGCAGGGAGTCAGCTTGCTGTCTCCTCGTGGTGCCGTCTCACTCGATTCGCGCACCAATGCAA
TCATCATTCGTGATATCGAGAAGAACATCGATCGTATTCGCACGTTAGTGAGTCATTTAGATGTGCCGGTCAAGCAAGTC
GAGATAGAGGCACGCATTGTGACTGTTGAGCAAGGCTCAGTGGATGAACTCGGTGTGCGCTGGGGGCTATTGAATCGAAA
TGGCTCAACCACGGTTGGTCCGTCTGTGGAAGGTAACTTGATGTGGGATAGCAATGTGACGGGGCAAGGGCCTGAAAGCT
TAAACGAGGCGGCGACAGACGGCGTAAATATGGGGGACTTTCTCAATGTGAACCTCGCGGCTGCGAGCCCTTCCGCCTCA
AGCATGGCGATGCAAGTGGCGAAGCTCGGCAATGACCTTCTACTCGATTTGGAGCTGTCGGCACTTGAAAGTGAAAGGAA
AGCAGAAGTGGTATCCAGCCCTCGTCTGATCACCACCAATAAAAAAGCGGCCTACATTGAACAAGGGACAGAGCTGCCTT
ACCTTGAGGCGTCTTCAAGCGGTGCGGTGGCGGTGTCGTTTAAAAAAGCGGTGTTGAGCTTATCGGTGACCCCACAAATT
ACGCCCAACAATACGCTGGTCTTGGATTTAAAGTTAACCCAAGATATGCCCGAAAAAGCGGTGGTGGCCGGAAAAGGCGA
AGCGATGTCGATCAGTACCAAGCGCATTAATACCCAAGTGTTGGCGGAAAATGGTGAAACTGTCGTCTTAGGGGGGATCT
TTCAGCAAACCATGATCAACAAAGAGACCCAAGTGCCGCTGTTGGGGGATATTCCGTTGGTTGGTAACCTGTTTAAGCAC
CAGTTAAAAGACCATGCAAAACGTGAACTTCTGATCTTTGTGACCCCTAAAATCGTCACAGACTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(421-579)

Bacterial type II/III secretion system short domain (Secretin_N)

(253-319)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

49.147

100

0.496
  pilQ Vibrio cholerae strain A1552

49.147

100

0.496
  pilQ Vibrio campbellii strain DS40M4

51.625

95.353

0.492
  comQ Acinetobacter baylyi ADP1

39.777

92.599

0.368
  pilQ Acinetobacter baumannii strain A118

37.95

95.697

0.363
  pilQ Acinetobacter baumannii D1279779

37.95

95.697

0.363