Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   O4598_RS01140 Genome accession   NZ_CP114586
Coordinates   235612..237357 (+) Length   581 a.a.
NCBI ID   WP_269599868.1    Uniprot ID   -
Organism   Salinivibrio kushneri strain TGB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 230612..242357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4598_RS01120 (O4598_01120) - 233015..233911 (+) 897 WP_077659173.1 hypothetical protein -
  O4598_RS01125 (O4598_01125) - 233908..234489 (+) 582 WP_077608179.1 PilN domain-containing protein -
  O4598_RS01130 (O4598_01130) - 234486..235067 (+) 582 WP_269587303.1 type 4a pilus biogenesis protein PilO -
  O4598_RS01135 (O4598_01135) - 235088..235615 (+) 528 WP_269599867.1 pilus assembly protein PilP -
  O4598_RS01140 (O4598_01140) pilQ 235612..237357 (+) 1746 WP_269599868.1 type IV pilus secretin PilQ Machinery gene
  O4598_RS01145 (O4598_01145) aroK 237502..238020 (+) 519 WP_025739049.1 shikimate kinase AroK -
  O4598_RS01150 (O4598_01150) aroB 238040..239119 (+) 1080 WP_069361857.1 3-dehydroquinate synthase -
  O4598_RS01155 (O4598_01155) - 239131..240756 (+) 1626 WP_269599869.1 AAA family ATPase -
  O4598_RS01160 (O4598_01160) dam 240829..241659 (+) 831 WP_077485800.1 adenine-specific DNA-methyltransferase -
  O4598_RS01165 (O4598_01165) - 241681..241869 (-) 189 WP_069361854.1 DUF2970 domain-containing protein -

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 62718.22 Da        Isoelectric Point: 5.2599

>NTDB_id=766615 O4598_RS01140 WP_269599868.1 235612..237357(+) (pilQ) [Salinivibrio kushneri strain TGB4]
MKASRVKRTLGSGLAQAVIVTVFVCGMVWSTVVGAAEAVTLVQAQLTSPTPQETQISLRFDSASVMADFRQLSPKQLMIR
LPQVVVKPDLLGRMKGQETGLPVNAVHITQVAGYTKIEIDAQQAMTFDYQQQHQQLIITLRDADPATAQAPDPDAPISIN
FQDIPVRAVLQLIAEQNQFNLVTTDSVQGNITMRIDGVPWQDVLDVILRVKGLDKRQRGDVMLVAPRKELMAQEKQDLEM
AQQADALLPLSSAVITVNYAKAADVAALIRGQGQGVSLLSPRGAVSLDSRTNAIIIRDIEKNIDRIRTLVSHLDVPVKQV
EIEARIVTVEQGSVDELGVRWGLLNRNGSTTVGPSVEGNLMWDSNMTGQGPESLNEAATDGVNMGDFLNVNLAAASPSAS
SMAMQVAKLGNDLLLDLELSALESERKAEVVSSPRLITTNKKAAYIEQGTELPYLEASSSGAVAVSFKKAVLSLSVTPQI
TPNNTLVLDLKLTQDMPEKAVVAGKGEAMSISTKRINTQVLAENGETVVLGGIFQQTMINKETQVPLLGDIPLVGNLFKH
QLKDHAKRELLIFVTPKIVTG

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=766615 O4598_RS01140 WP_269599868.1 235612..237357(+) (pilQ) [Salinivibrio kushneri strain TGB4]
ATGAAAGCAAGCAGGGTAAAACGCACACTCGGATCAGGACTGGCGCAAGCTGTCATTGTGACTGTTTTCGTTTGTGGGAT
GGTATGGAGTACCGTTGTGGGGGCCGCTGAGGCAGTGACTTTAGTGCAGGCGCAACTTACGTCTCCCACGCCGCAAGAGA
CGCAGATCAGCTTACGTTTTGACAGTGCCTCGGTGATGGCGGACTTTCGCCAGTTGAGCCCTAAGCAGCTTATGATTCGA
TTACCTCAGGTGGTCGTCAAGCCAGACTTACTTGGGCGGATGAAAGGGCAAGAGACGGGGTTGCCTGTGAACGCCGTTCA
CATCACCCAGGTCGCAGGCTACACCAAGATAGAAATTGATGCCCAGCAAGCGATGACGTTTGATTACCAACAACAGCATC
AGCAATTAATTATCACCTTGCGCGACGCCGATCCGGCGACCGCCCAAGCCCCTGATCCCGACGCACCGATTTCCATCAAT
TTCCAAGATATTCCTGTGCGGGCGGTGCTACAACTGATTGCCGAACAAAATCAATTTAATTTGGTGACCACTGACAGTGT
GCAGGGCAATATCACCATGCGTATCGACGGGGTACCCTGGCAAGATGTGTTGGATGTGATTCTCAGGGTCAAGGGGTTAG
ACAAGCGCCAACGTGGGGATGTGATGTTAGTGGCCCCTCGTAAAGAACTGATGGCGCAAGAAAAACAAGATCTCGAGATG
GCGCAACAAGCAGACGCGCTGTTGCCTTTGTCTTCTGCCGTGATCACCGTTAACTACGCCAAAGCCGCTGACGTCGCGGC
GTTAATCCGAGGCCAGGGGCAGGGAGTCAGCTTGCTGTCTCCTCGTGGTGCCGTCTCACTCGATTCGCGCACCAATGCGA
TCATCATTCGTGATATCGAGAAGAACATCGATCGTATTCGCACGTTAGTGAGTCATTTAGATGTGCCGGTTAAGCAAGTC
GAGATAGAGGCACGCATTGTGACTGTTGAGCAAGGCTCAGTGGATGAACTCGGTGTGCGCTGGGGGCTATTGAATCGAAA
TGGCTCAACCACGGTTGGTCCGTCTGTGGAAGGTAACTTGATGTGGGATAGCAATATGACGGGGCAAGGGCCTGAAAGCT
TAAACGAGGCGGCGACAGACGGCGTAAATATGGGGGACTTTCTCAATGTGAACCTCGCGGCTGCGAGCCCTTCCGCCTCA
AGCATGGCGATGCAAGTGGCGAAGCTCGGCAATGACCTTCTACTCGATTTGGAGCTGTCGGCACTTGAAAGTGAAAGGAA
AGCAGAAGTGGTATCCAGCCCTCGTCTGATCACCACCAATAAAAAAGCGGCCTACATTGAACAAGGGACAGAGCTGCCTT
ACCTTGAGGCGTCTTCAAGCGGTGCGGTGGCGGTGTCGTTTAAAAAAGCGGTGTTGAGCTTATCGGTAACCCCACAAATT
ACGCCCAACAATACGCTGGTCTTGGATTTAAAGTTAACCCAAGATATGCCCGAAAAAGCGGTAGTGGCCGGAAAAGGCGA
AGCGATGTCGATCAGTACCAAGCGCATTAATACCCAAGTGTTGGCGGAAAATGGTGAAACTGTCGTCTTAGGGGGGATCT
TTCAGCAAACCATGATCAACAAAGAGACCCAGGTGCCGCTGCTGGGGGATATTCCGTTGGTTGGTAACCTGTTTAAGCAC
CAGTTAAAAGACCATGCAAAACGCGAACTTCTGATCTTTGTGACCCCTAAAATCGTCACAGGCTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(253-319)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

48.976

100

0.494
  pilQ Vibrio cholerae strain A1552

48.976

100

0.494
  pilQ Vibrio campbellii strain DS40M4

54.42

87.608

0.477
  comQ Acinetobacter baylyi ADP1

39.739

92.255

0.367