Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   QIH85_RS08120 Genome accession   NZ_CP126010
Coordinates   1749224..1750678 (+) Length   484 a.a.
NCBI ID   WP_011084688.1    Uniprot ID   P30332
Organism   Bradyrhizobium japonicum strain N03G-Bj     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1744224..1755678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QIH85_RS08105 (QIH85_08105) - 1744431..1745045 (+) 615 WP_011084684.1 J domain-containing protein -
  QIH85_RS08110 (QIH85_08110) - 1746145..1746930 (+) 786 WP_018645272.1 LuxR family transcriptional regulator -
  QIH85_RS08115 (QIH85_08115) - 1747753..1747941 (-) 189 Protein_1612 hypothetical protein -
  QIH85_RS08120 (QIH85_08120) rpoN/rpoN1 1749224..1750678 (+) 1455 WP_011084688.1 RNA polymerase factor sigma-54 Machinery gene
  QIH85_RS08125 (QIH85_08125) - 1751363..1751701 (-) 339 WP_275937772.1 hypothetical protein -
  QIH85_RS08130 (QIH85_08130) - 1751774..1751944 (+) 171 WP_014497906.1 hypothetical protein -
  QIH85_RS08135 (QIH85_08135) - 1752502..1752846 (+) 345 WP_085973967.1 hypothetical protein -
  QIH85_RS08140 (QIH85_08140) - 1752843..1753205 (+) 363 WP_026312353.1 Wadjet anti-phage system protein JetD domain-containing protein -
  QIH85_RS08145 (QIH85_08145) - 1753285..1753510 (+) 226 Protein_1618 IS5/IS1182 family transposase -
  QIH85_RS08150 (QIH85_08150) - 1753585..1753803 (-) 219 WP_161966258.1 hypothetical protein -
  QIH85_RS08155 (QIH85_08155) - 1754163..1755548 (+) 1386 WP_011084694.1 sugar ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 484 a.a.        Molecular weight: 53772.80 Da        Isoelectric Point: 4.7595

>NTDB_id=766223 QIH85_RS08120 WP_011084688.1 1749224..1750678(+) (rpoN/rpoN1) [Bradyrhizobium japonicum strain N03G-Bj]
MALTQRLEFRQSQSLVMSPQLMQAIKLLQLSNLDLMTFVEEELECNPLLERASDDAAGAEAPTEVDQVSGDQLAEAQVRD
ARDGAMTTYTEWGGGGSGDEDYNLEAFVASETTLSDHLAEQLSVAFTAPAQRMIGQYLIDLVDEAGYLPPDLGQAAERLG
ATQEDVEHVLAVLQEFDPPGVCARNLRECLAIQLRELDRYDPAMQALVEHLDLLAKRDIASLRKLCGVDDEDIADMIDEL
RRLSPKPGMKFGSARLQTMVPDVYVRPAPDGGWHVELNSDTLPRVLVNQTYYSKLSKKIGKDVDKSYFNDALQNATWLVR
ALDQRARTILKVATEIVRQQDGFFTLGVAHLRPLNLKAVAEAIQMHESTVSRVTANKYMATNRGTFELKYFFTASIPSAD
GGEAHSAEAVRHRIKQLIESEEPSAVLSDDAIVERLRVSGIDIARRTVAKYREAMRIRSSVQRRRDNMWSTMNSRASGGT
GLDK

Nucleotide


Download         Length: 1455 bp        

>NTDB_id=766223 QIH85_RS08120 WP_011084688.1 1749224..1750678(+) (rpoN/rpoN1) [Bradyrhizobium japonicum strain N03G-Bj]
ATGGCGCTCACGCAAAGATTAGAGTTCCGGCAATCCCAGTCGCTGGTCATGTCGCCGCAGCTGATGCAGGCGATCAAGCT
GCTGCAATTATCTAATCTCGACCTCATGACCTTCGTGGAGGAAGAGCTGGAGTGTAATCCGCTGCTCGAGCGTGCCAGTG
ACGATGCAGCTGGGGCCGAAGCCCCGACTGAGGTCGATCAGGTCAGCGGCGATCAGCTGGCCGAGGCCCAAGTGCGCGAC
GCCCGGGATGGCGCCATGACCACCTATACCGAATGGGGTGGCGGCGGCTCGGGTGACGAAGACTACAATCTCGAAGCGTT
TGTCGCGTCCGAGACAACATTGTCCGACCACCTGGCCGAACAACTGTCGGTCGCATTCACCGCGCCGGCGCAGCGCATGA
TCGGGCAGTATCTGATCGATCTCGTCGACGAAGCCGGCTATCTGCCGCCGGATCTCGGCCAGGCCGCCGAGCGCCTCGGC
GCAACGCAGGAGGATGTCGAGCACGTTCTTGCCGTCCTGCAGGAGTTCGATCCGCCCGGCGTCTGTGCGCGTAACTTGCG
CGAGTGCCTGGCGATCCAGCTCCGCGAGCTCGATAGATACGATCCGGCGATGCAGGCCCTCGTCGAGCATCTCGATCTCC
TCGCCAAGCGCGACATCGCGAGCTTGCGCAAGCTCTGCGGCGTCGACGACGAGGACATCGCCGACATGATCGACGAGCTC
CGCCGGCTCAGTCCCAAGCCGGGCATGAAGTTCGGGTCGGCGCGGCTGCAGACGATGGTACCCGACGTCTATGTCCGTCC
GGCTCCTGATGGCGGCTGGCATGTCGAGCTCAACAGCGACACCTTGCCGCGCGTGCTGGTCAACCAGACCTATTATTCCA
AGCTGTCGAAGAAGATCGGCAAGGACGTCGATAAGTCCTACTTCAACGACGCGCTGCAGAACGCGACCTGGCTGGTGCGC
GCGCTCGACCAGCGCGCCCGCACCATCCTGAAAGTTGCGACCGAGATCGTGCGTCAGCAGGACGGCTTCTTTACCCTTGG
TGTTGCGCATTTGCGGCCGCTGAATCTAAAGGCCGTTGCCGAGGCCATCCAGATGCATGAATCCACGGTGTCGCGCGTCA
CCGCCAACAAATACATGGCAACAAATCGCGGCACATTCGAGTTGAAATATTTCTTCACGGCATCGATCCCTTCGGCGGAT
GGCGGTGAGGCGCATTCCGCTGAAGCGGTGCGTCACCGCATCAAGCAGCTGATCGAATCCGAAGAGCCGTCAGCGGTTCT
GTCCGATGACGCGATCGTTGAGCGCCTGCGAGTCTCGGGCATTGATATTGCCCGCCGCACGGTCGCGAAGTACCGCGAAG
CCATGCGCATTCGGTCCTCGGTGCAGCGCCGCCGCGACAATATGTGGTCAACGATGAACAGTAGAGCATCGGGCGGAACT
GGCCTCGATAAATAA

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(6-50)

Sigma-54, DNA binding domain (Sigma54_DBD)

(306-465)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(104-291)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P30332

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

38.337

100

0.39