Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   O2U05_RS03645 Genome accession   NZ_CP114519
Coordinates   559363..560736 (+) Length   457 a.a.
NCBI ID   WP_003702635.1    Uniprot ID   F5VC94
Organism   Ligilactobacillus salivarius strain S91     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 554363..565736
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O2U05_RS03615 (O2U05_03610) - 554584..555528 (+) 945 WP_003700695.1 AEC family transporter -
  O2U05_RS03620 (O2U05_03615) - 555550..556224 (+) 675 WP_283472285.1 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase -
  O2U05_RS03625 (O2U05_03620) - 556389..556742 (-) 354 WP_003710547.1 bacteriocin immunity protein -
  O2U05_RS03630 (O2U05_03625) - 556952..558286 (-) 1335 WP_003706508.1 C1 family peptidase -
  O2U05_RS03635 (O2U05_03630) - 558375..558653 (-) 279 WP_283472290.1 GNAT family N-acetyltransferase -
  O2U05_RS03640 (O2U05_03635) - 558809..559345 (+) 537 WP_283472293.1 dUTP diphosphatase -
  O2U05_RS03645 (O2U05_03640) radA 559363..560736 (+) 1374 WP_003702635.1 DNA repair protein RadA Machinery gene
  O2U05_RS03650 (O2U05_03645) - 560762..561874 (+) 1113 WP_003700688.1 PIN/TRAM domain-containing protein -
  O2U05_RS03655 (O2U05_03650) gltX 562005..563492 (+) 1488 WP_283472300.1 glutamate--tRNA ligase -
  O2U05_RS03660 (O2U05_03655) cysS 563644..565056 (+) 1413 WP_283472302.1 cysteine--tRNA ligase -
  O2U05_RS03665 (O2U05_03660) - 565049..565456 (+) 408 WP_003700685.1 Mini-ribonuclease 3 -

Sequence

Protein


Download         Length: 457 a.a.        Molecular weight: 49722.37 Da        Isoelectric Point: 8.0254

>NTDB_id=766216 O2U05_RS03645 WP_003702635.1 559363..560736(+) (radA) [Ligilactobacillus salivarius strain S91]
MAKTKTRYVCQNCEYISPRYLGRCPNCGSWNSLVEEIEKKESSTKAQPRVSISGTTVKPKLIDDVSTVETPRFKTNLEEL
NRVLGGGIVPGSLVLIGGDPGIGKSTLLLQVSGQLSTLGKKVLYVSGEESATQIKLRADRLGVHGTELYLYPETDMDSIR
KNIELLKPDFVVIDSVQTMQEPEMTAAVGSVSQIREVTGDLMQIAKTNGITVFVVGHVTKGGAIAGPKILEHMVDTVLYF
EGDQHRTFRILRAVKNRFGSTNEIGIFEMKNGGLSEVANPSEIFLEERLKGATGSAVVASLEGTRPILVELQALVTPTAF
GNAKRTTTGLDHNRVALLMAVLEKRAGLLLQNQDAYLKAAGGVKLDEPAIDLAIAVSLVSSYRDAETRPTDCFIGEVGLT
GEIRRVNRIEQRVVEAAKLGFKRVFLPKNNLDGWMPPKEIEVVGVSTLNQALKLIFS

Nucleotide


Download         Length: 1374 bp        

>NTDB_id=766216 O2U05_RS03645 WP_003702635.1 559363..560736(+) (radA) [Ligilactobacillus salivarius strain S91]
TTGGCGAAAACTAAGACAAGATATGTTTGTCAAAATTGTGAATATATCTCTCCACGTTATTTAGGACGCTGCCCTAATTG
TGGTTCTTGGAATAGCTTGGTAGAGGAAATAGAGAAAAAGGAAAGTTCAACTAAAGCACAGCCTAGAGTTAGCATCTCTG
GTACAACAGTGAAACCTAAATTAATTGATGATGTTTCTACTGTGGAAACTCCAAGATTTAAAACTAATTTAGAAGAATTG
AATCGTGTTTTAGGTGGCGGAATTGTTCCCGGATCTTTAGTGTTAATTGGTGGAGATCCAGGTATTGGCAAGTCTACATT
ATTACTACAAGTTTCTGGACAATTATCTACTCTTGGGAAAAAGGTTCTTTATGTTTCTGGGGAAGAAAGTGCAACTCAAA
TTAAATTACGTGCGGATCGTTTAGGAGTTCATGGAACTGAGCTATATTTATATCCTGAAACAGATATGGATAGTATTAGA
AAAAATATTGAACTTTTAAAGCCTGATTTTGTTGTAATCGATTCGGTGCAGACAATGCAGGAGCCAGAAATGACAGCCGC
AGTTGGAAGTGTTTCTCAAATCAGGGAAGTAACTGGAGATTTGATGCAAATTGCGAAAACTAATGGGATAACTGTCTTTG
TTGTAGGACATGTTACTAAAGGGGGAGCAATTGCAGGACCTAAAATTTTAGAACACATGGTAGATACTGTTCTTTATTTT
GAGGGAGATCAACATAGAACATTTAGAATTTTACGAGCGGTTAAAAATAGATTTGGTTCAACTAATGAAATTGGAATTTT
TGAAATGAAAAATGGAGGATTAAGTGAAGTTGCTAATCCATCAGAGATTTTTCTAGAAGAAAGATTAAAAGGAGCTACGG
GATCAGCAGTCGTTGCTTCGCTTGAAGGGACTAGGCCTATATTGGTAGAATTACAAGCACTAGTAACACCGACTGCATTT
GGAAATGCTAAGCGAACAACAACTGGTTTAGATCATAATCGAGTTGCGTTACTGATGGCGGTACTTGAAAAAAGAGCTGG
TTTGTTATTACAAAATCAGGATGCTTATTTGAAGGCTGCTGGTGGAGTGAAGTTAGATGAGCCAGCAATAGATTTAGCCA
TTGCTGTTAGTTTAGTTTCTAGTTATCGCGATGCAGAAACAAGACCAACTGACTGTTTTATAGGGGAAGTGGGATTAACT
GGTGAAATAAGACGAGTTAATCGTATTGAACAGCGTGTTGTTGAGGCTGCTAAGCTAGGATTTAAGCGAGTATTTTTACC
AAAGAACAACTTAGATGGTTGGATGCCTCCAAAAGAGATTGAAGTAGTAGGCGTAAGTACACTAAATCAAGCATTAAAAC
TAATTTTTAGTTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-430)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-222)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5VC94

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.49

100

0.685
  radA Streptococcus mitis SK321

68.271

100

0.683
  radA Streptococcus pneumoniae Rx1

68.271

100

0.683
  radA Streptococcus pneumoniae D39

68.271

100

0.683
  radA Streptococcus pneumoniae R6

68.271

100

0.683
  radA Streptococcus pneumoniae TIGR4

68.271

100

0.683
  radA Bacillus subtilis subsp. subtilis str. 168

65.563

99.125

0.65