Detailed information NTG0765946

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: O3S71_RS20755
• Genome accession: NZ_CP114406
• Coordinates: 4013224..4016949 (-)
• Length: 1241 a.a.
• NCBI ID: WP_016077977.1
• Uniprot ID: A0A9X6Z472
• Organism: Bacillus thuringiensis serovar tenebrionis strain NB-176
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 4008224..4021949

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
O3S71_RS20705	-	4009059..4009343 (+)	285	WP_016077976.1	hypothetical protein	-
O3S71_RS20710	-	4009379..4010278 (-)	900	WP_001213078.1	fumarylacetoacetate hydrolase family protein	-
O3S71_RS20715	-	4010525..4010704 (+)	180	WP_000462848.1	aspartyl-phosphate phosphatase Spo0E family protein	-
O3S71_RS20720	gerPA	4010801..4011022 (+)	222	WP_001111188.1	spore germination protein GerPA	-
O3S71_RS20725	gerPB	4011037..4011243 (+)	207	WP_001012509.1	spore germination protein GerPB	-
O3S71_RS20730	gerPC	4011311..4011925 (+)	615	WP_001070761.1	spore germination protein GerPC	-
O3S71_RS20735	gerPD	4011932..4012126 (+)	195	WP_001052807.1	spore germination protein GerPD	-
O3S71_RS20740	-	4012142..4012528 (+)	387	WP_000902327.1	spore germination protein GerPE	-
O3S71_RS20745	gerPF	4012571..4012786 (+)	216	WP_001141566.1	spore germination protein GerPF	-
O3S71_RS20750	-	4012924..4013211 (-)	288	WP_000255718.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
O3S71_RS20755	addA	4013224..4016949 (-)	3726	WP_016077977.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
O3S71_RS20760	addB	4016946..4020461 (-)	3516	WP_016077978.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
O3S71_RS20765	lepB	4020577..4021140 (-)	564	WP_000751896.1	signal peptidase I	-
O3S71_RS20770	-	4021197..4021790 (-)	594	WP_000347517.1	TVP38/TMEM64 family protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142800.96 Da
• Isoelectric Point: 4.7855

>NTDB_id=765946 O3S71_RS20755 WP_016077977.1 4013224..4016949(-) (addA) [Bacillus thuringiensis serovar tenebrionis strain NB-176]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADVALLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMNPSAVALQ
YRNKFTEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEDLATLRAHGKKGSFYEVMSSFLKGAPLEEEQELHEKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTNWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEDATSYRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSEETILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EDRYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVNIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=765946 O3S71_RS20755 WP_016077977.1 4013224..4016949(-) (addA) [Bacillus thuringiensis serovar tenebrionis strain NB-176]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCTGTTGTAGCGAACGGACG
TGATATTTTAGTTGCGGCTGCAGCTGGATCAGGTAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAAAATCGAATT
GGGGAAGCGTTAGAAAAGGTATTAATTGATGAACCAGGCTCTCAGCATGTAAGAAAGCAACTGAGCCTATTAAATAAAGC
TTCCATTTCAACAATTCACTCATTCTGTTTACAAGTAATTAGAGGATATTACTACATGCTGGATGTTGATCCTCGTTTCC
GTATTGCGAATCAAACAGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTACGGAATCGAA
GATAATACAATCTTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGATGATGACTTACAACGTATGATTTTAGC
GCTTCATACAGAGTCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGACGTAGAAGGAA
AGACAATTGAAGATTTAGTATATGCTTCTTATTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAGGCAACTGAACTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATGTAGCATTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAAGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTA
AAGAAATTACAAGAAGAGTTATTTAGCCGCAAACCTGAAAGTTTCTTACGAGATTTTCAAGATATGCACCCAGTATTAGA
AAAGCTTGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAATCCGTCAGCAGTGGCGCTCCAA
TATCGTAATAAATTTACTGAAGTATTAGTCGATGAATATCAAGATACGAACTTTGTACAGGAATCCATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAGCAGTCGATTTATCGTTTCCGACTAGCTG
AACCAGGACTATTCCTAGGAAAGTATAAACGCTTCACACAAGAAGGATTGGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTACTAGCAGGTACGAACTTCATTTTCAAACAAATTATGGGCGAAGAAGTTGGAGAGAT
TGACTACGATGCTGACGCTGAATTAAAACTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCAGAATTACTCTGTA
TTCAGCAAACGGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCAGAAGTCGAAAAAGCACAGCTTGAAGCTCGCCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCCGGTTATGAAGTGTATGACCGTAAAACGGATAGTATGCGTCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCGCCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCGG
TATATGCTGACCTTGCGACTGGTTATTTTGAAGCAACGGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATCCCGCTTGCAGCTGTACTTCGTTCCCCAATCGTTGGATTAAACGATGAAGACCTTGCGACGCTTCG
TGCTCACGGAAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGTGCACCGCTTGAAGAGGAACAAGAAC
TTCATGAAAAACTAGAGTGGTTTTATAACTTACTGCAAGGATGGCGTGAATTTGCACGTCAACAATCACTTTCTGATTTA
ATTTGGAAAGTGTACGGCGAGACAGGTTATTACGACTTCGTTGGCGGTTTACCAGCTGGAAAGCAAAGGCAGGCAAACTT
ACGCGTACTATATGACCGTGCAAGACAATATGAAGCAACATCATTTAGAGGATTATTCCGCTTCTTACGTTTTATTGAAC
GTATTTTAGAACGCGGTGATGATATGGGGACGGCGAGGGCCCTCGGTGAACAAGAAGACGTTGTTCGCATCATGACGATC
CATAAAAGTAAAGGGCTAGAGTTCCCGGTTGTATTTGTAGCTGGACTCGGCCGTCGTTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTCCATAAAGATTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATACACGACATTAT
CACAGCTTGCGATTAAACGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTACTATACGTAGCGTTGACGCGT
GCGAAAGAGAAGTTAATTTTAATCGGAACAGTTAAGGATGCAAATAAGGAAATGGAAAAATGGCTTGATGCGAGGGAACA
TAGTGAATGGTTATTACCGGATCACATACGTGCCGGAGCGTCTTGTTATTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGACAAGGAAGTATTCCAGATGAAATTTACGGGTATGACACGAACTGGAAA
GTAGAAGTTGTGGACGGTAACACGCTACTTGCACCAGAGCCAGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCGCTTCG
TGAGAAAAAGGCTGTTCCCCTGCAAAGTGAACGGAAAGAAGAAGTGTACGACAGATTAATGTGGAAGTACGGATATGAGG
ATGCAACATCTTATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAACTACGTGCGCCAATTAAAACACGTCCGCGCTTTATGGAGAAAAAAGGATTAACGTACGCAGAGCGTGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGGTTGAAGTTCTGCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTCGAGCAGGCGGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GATTTAGGTAAAAGGGTACTAGCGGCGAAAAGTGTTGAGCGTGAAGTGCCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGTGAAGAAACGATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGACG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGCGGATTCGAACAAGCAAAACCAATTTTA
GAAGATAGATATAAAGTACAGCTTTCGTTATATGCAAAAGCGCTGGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGGAATCATGTTGTAAATATTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.168	100	0.534