Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OGU21_RS03960 Genome accession   NZ_CP114305
Coordinates   820430..821410 (+) Length   326 a.a.
NCBI ID   WP_004105710.1    Uniprot ID   A0A9P0U2X2
Organism   Klebsiella oxytoca strain 2022CK-00498     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 815430..826410
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OGU21_RS03930 (OGU21_03935) - 815812..816798 (-) 987 WP_004115390.1 TRAP transporter substrate-binding protein -
  OGU21_RS03935 (OGU21_03940) hemW 817103..818239 (-) 1137 WP_004105720.1 radical SAM family heme chaperone HemW -
  OGU21_RS03940 (OGU21_03945) - 818232..818825 (-) 594 WP_004105717.1 XTP/dITP diphosphatase -
  OGU21_RS03945 (OGU21_03950) yggU 818838..819128 (-) 291 WP_004105716.1 DUF167 family protein YggU -
  OGU21_RS03950 (OGU21_03955) - 819125..819691 (-) 567 WP_004105714.1 YggT family protein -
  OGU21_RS03955 (OGU21_03960) - 819709..820413 (-) 705 WP_016807917.1 YggS family pyridoxal phosphate-dependent enzyme -
  OGU21_RS03960 (OGU21_03965) pilT 820430..821410 (+) 981 WP_004105710.1 type IV pilus twitching motility protein PilT Machinery gene
  OGU21_RS03965 (OGU21_03970) - 821759..822412 (+) 654 WP_004105708.1 LuxR C-terminal-related transcriptional regulator -
  OGU21_RS03970 (OGU21_03975) ruvX 822468..822884 (-) 417 WP_004105707.1 Holliday junction resolvase RuvX -
  OGU21_RS03975 (OGU21_03980) - 822884..823447 (-) 564 WP_004105705.1 YqgE/AlgH family protein -
  OGU21_RS03980 (OGU21_03985) gshB 823624..824571 (-) 948 WP_016807916.1 glutathione synthase -
  OGU21_RS03985 (OGU21_03990) rsmE 824592..825323 (-) 732 WP_004105702.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OGU21_RS03990 (OGU21_03995) endA 825415..826122 (-) 708 WP_004115375.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35124.23 Da        Isoelectric Point: 6.7168

>NTDB_id=765433 OGU21_RS03960 WP_004105710.1 820430..821410(+) (pilT) [Klebsiella oxytoca strain 2022CK-00498]
MELEEIVALSVKHNVSDLHLCNASAPRWRRQGKLEPAPFTSPDISKILHDWLNAEQLAQWQASGQVDFALAPAGGPRLRA
SAFAHSRGLSLALRLLAETCPQLEALGVPAALSELLNEESGLILVTGATGSGKSTTLAAMVGYLNQRLNGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFAAALRAALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAQAVERLVDV
FPAQEKEQVRSQLAGSLCAVLAQKLFPDAAGGRVALYELLVNTPAVANLIREGKTHQLPGVMQTGQQSGMQTFTQSFQQR
TAAGLL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=765433 OGU21_RS03960 WP_004105710.1 820430..821410(+) (pilT) [Klebsiella oxytoca strain 2022CK-00498]
ATGGAACTGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAATGCTTCAGCGCCGCG
CTGGCGTCGCCAGGGAAAGCTGGAGCCTGCGCCTTTTACCTCCCCTGATATCAGCAAAATACTCCATGACTGGCTCAACG
CCGAGCAGCTCGCGCAGTGGCAGGCCAGCGGGCAGGTCGATTTCGCTTTAGCGCCGGCTGGCGGGCCACGCCTTCGCGCC
AGCGCATTTGCCCACAGCCGCGGCCTGTCGCTGGCGCTGCGTCTGCTGGCGGAAACCTGCCCGCAGCTGGAGGCGCTCGG
TGTACCAGCCGCCTTAAGCGAGCTGCTCAATGAGGAGAGCGGGCTTATCCTCGTCACGGGCGCCACCGGCAGCGGGAAAT
CAACTACCCTGGCGGCGATGGTGGGCTATCTTAATCAGCGCCTCAATGGTCATATCCTGACGCTGGAAGATCCGGTCGAA
TTTATTCACCACAGCGAGCGTTGCCTGATCCAGCAGCGGGAAATCGGCCGCCACTGCCCCTCCTTTGCCGCGGCGTTACG
CGCTGCGCTGCGTCAGGATCCGGACGTCATTCTGTTAGGGGAACTGCGCGATAGCGAAACTATCCGCCTGGCACTTACGG
CAGCGGAGACCGGACATCTGGTTATGGCGACGCTGCATACGCGCGGCGCGGCGCAGGCGGTTGAAAGGCTGGTGGATGTC
TTTCCCGCCCAGGAGAAAGAGCAGGTTCGCAGCCAGCTGGCGGGAAGTTTGTGCGCGGTACTGGCACAAAAATTGTTTCC
GGATGCTGCAGGCGGCAGAGTTGCGCTATACGAGCTTTTAGTTAATACCCCAGCAGTGGCGAATTTGATCCGGGAAGGAA
AAACGCATCAGCTGCCCGGCGTGATGCAAACCGGGCAGCAATCTGGCATGCAAACATTTACGCAAAGCTTTCAGCAACGT
ACGGCAGCGGGTCTTTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.152

100

0.506
  pilT Vibrio cholerae strain A1552

50.152

100

0.506
  pilT Neisseria meningitidis 8013

48.182

100

0.488
  pilT Neisseria gonorrhoeae MS11

47.879

100

0.485
  pilT Legionella pneumophila strain Lp02

48.428

97.546

0.472
  pilT Legionella pneumophila strain ERS1305867

48.428

97.546

0.472
  pilT Pseudomonas stutzeri DSM 10701

45.897

100

0.463
  pilT Pseudomonas aeruginosa PAK

45.593

100

0.46
  pilT Acinetobacter baumannii strain A118

45.152

100

0.457
  pilT Acinetobacter baumannii D1279779

45.152

100

0.457
  pilT Acinetobacter baylyi ADP1

45.289

100

0.457
  pilT Acinetobacter nosocomialis M2

44.848

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.479

97.239

0.433
  pilU Pseudomonas stutzeri DSM 10701

41.463

100

0.417
  pilU Vibrio cholerae strain A1552

41.304

98.773

0.408
  pilU Acinetobacter baylyi ADP1

37.888

98.773

0.374