Detailed information    

experimental Experimentally validated

Overview

Name   comH   Type   Machinery gene
Locus tag   C694_RS07860 Genome accession   NC_018939
Coordinates   1605979..1607418 (-) Length   479 a.a.
NCBI ID   WP_000749005.1    Uniprot ID   A0AAC8N2R8
Organism   Helicobacter pylori 26695     
Function   import of DNA into the periplasm   
DNA binding and uptake

Function

ComH is a DNA-binding protein required for the import of DNA into the periplasm. Its C-terminal domain displays strong affinity for double-stranded DNA and is sufficient for the accumulation of DNA in the periplasm, but not for DNA internalisation into the cytoplasm. The N-terminal region of the protein allows the interaction of ComH with a periplasmic domain of the inner-membrane channel ComEC, which is known to mediate the translocation of DNA into the cytoplasm.


Genomic Context

Location: 1600979..1612418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C694_RS07835 (C694_07890) recG 1602111..1603982 (-) 1872 WP_001158370.1 ATP-dependent DNA helicase RecG -
  C694_RS07840 (C694_07895) - 1604064..1604411 (+) 348 WP_000837022.1 lipoprotein -
  C694_RS07845 (C694_07900) - 1604415..1605050 (+) 636 WP_000765634.1 outer membrane protein -
  C694_RS07850 (C694_07905) - 1605047..1605799 (-) 753 WP_000767556.1 exodeoxyribonuclease III -
  C694_RS07860 (C694_07910) comH 1605979..1607418 (-) 1440 WP_000749005.1 hypothetical protein Machinery gene
  C694_RS07870 (C694_07920) dnaA 1607647..1609020 (-) 1374 WP_000380647.1 chromosomal replication initiator protein DnaA -
  C694_RS07875 (C694_07925) - 1609173..1609715 (+) 543 WP_000923009.1 hypothetical protein -
  C694_RS07880 (C694_07930) - 1609758..1609997 (+) 240 WP_000461837.1 DUF2443 domain-containing protein -
  C694_RS07885 (C694_07935) glmS 1609998..1611791 (+) 1794 WP_000334435.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 54783.74 Da        Isoelectric Point: 7.0430

>NTDB_id=1207 C694_RS07860 WP_000749005.1 1605979..1607418(-) (comH) [Helicobacter pylori 26695]
MKKSLCLSFFLTFSNPLQALVIELLEEIKTSPHKGTFKAKVLDSKKPRQVLGVYNISPHKKLTLTITHISTAIVYQPLDE
KLSLETTLNPNRPTIPRNTQIVFSSKELKESHPHQMPSLNAPMQKPQNKPHSSQQPSQNFSYPEPKLGSKNSKNSLLQPL
AIPSKISPTNETQTPTNDTKPPLKHSSEDQESNLFITPPTEKTLPNNTSNADISENNESNENKDNVEKQAIRDANIKEFA
CGKWVYDDENLQAYRPSILKRVDEDKQTATDITPCDYSTAENKSGKIITPYTKISVHKTEPLEEPQTFEAKNNFAILQAR
SSTEKCKRARARKDGTTRQCYLIEEPLKQAWESEYEITTQLVKAIYERPKQDDQVEPTFYETSELAYSSTRKSEITHNEL
NLNEKFMEFVEVYEGHYLNDIIKESSEYKEWVKNHVRFKEGVCMALEIEEQPRAKSTPLSIENSRVVCVKKGNYLFNEV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=1207 C694_RS07860 WP_000749005.1 1605979..1607418(-) (comH) [Helicobacter pylori 26695]
ATGAAAAAATCCCTTTGTCTGTCTTTCTTTCTGACTTTCTCTAACCCTCTTCAAGCCCTTGTGATCGAGCTTTTAGAAGA
AATCAAAACTTCGCCGCATAAAGGCACTTTTAAGGCTAAAGTCCTTGATTCTAAAAAACCAAGACAAGTTTTAGGCGTTT
ATAATATCTCCCCACACAAAAAACTCACGCTCACTATCACCCACATATCCACTGCAATCGTCTATCAACCCCTTGATGAA
AAACTTTCTTTAGAAACAACCTTAAACCCTAACCGCCCTACTATCCCTAGAAACACCCAGATTGTTTTTTCTTCAAAAGA
ATTGAAAGAGTCGCACCCGCACCAAATGCCTTCTTTAAACGCGCCCATGCAAAAACCACAAAACAAACCCCATTCATCGC
AACAACCTTCTCAAAACTTTTCTTACCCAGAGCCCAAACTAGGCTCTAAAAACTCTAAAAACAGCCTTTTACAGCCTTTA
GCAATTCCTAGCAAAATAAGTCCCACTAACGAAACTCAAACGCCAACAAACGACACTAAACCCCCTTTAAAGCATTCTTC
AGAAGATCAAGAAAGCAACCTCTTTATAACGCCACCCACTGAAAAAACGCTCCCTAACAACACCTCTAACGCTGATATTA
GTGAAAACAATGAAAGCAATGAGAATAAAGATAATGTGGAAAAACAAGCCATTAGAGATGCTAATATTAAAGAATTTGCA
TGCGGGAAGTGGGTCTATGACGATGAAAATTTACAAGCCTACCGCCCAAGCATTTTAAAACGCGTTGATGAAGACAAACA
AACTGCAACAGATATTACCCCTTGCGATTACAGCACCGCTGAAAATAAAAGCGGTAAAATCATTACCCCCTATACTAAAA
TCTCCGTTCATAAAACAGAGCCTTTAGAAGAGCCACAAACTTTTGAAGCTAAAAATAATTTCGCCATTCTTCAAGCCAGA
AGCTCTACAGAAAAATGCAAAAGGGCTAGAGCAAGAAAAGACGGCACGACTAGGCAATGCTATCTAATAGAAGAGCCTTT
AAAACAAGCATGGGAGAGTGAGTATGAAATCACCACGCAATTAGTGAAAGCCATTTATGAGCGCCCCAAACAAGACGATC
AAGTAGAGCCGACTTTTTATGAAACCAGCGAATTGGCTTATTCTTCCACACGAAAAAGCGAAATAACGCACAATGAATTG
AATTTGAATGAAAAATTCATGGAATTTGTGGAAGTGTATGAGGGGCATTATTTAAACGATATAATTAAAGAGAGCAGTGA
ATATAAAGAATGGGTTAAAAACCATGTGCGCTTTAAAGAAGGGGTGTGCATGGCTTTAGAAATAGAAGAACAGCCACGAG
CTAAAAGCACGCCTTTGAGTATTGAAAACTCTCGTGTGGTATGTGTCAAAAAGGGGAATTATTTATTCAACGAAGTTTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] Prashant P Damke et al. (2019) Identification of the periplasmic DNA receptor for natural transformation of Helicobacter pylori. Nature Communications 10(1):5357. [PMID: 31767852]
[2] L C Smeets et al. (2000) comH, a novel gene essential for natural transformation of Helicobacter pylori. Journal of Bacteriology 182(14):3948-54. [PMID: 10869072]