Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   O0R49_RS12180 Genome accession   NZ_CP114177
Coordinates   2349009..2350079 (-) Length   356 a.a.
NCBI ID   WP_269195351.1    Uniprot ID   -
Organism   Bacillus safensis strain PLA 1006     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2344009..2355079
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O0R49_RS12130 (O0R49_12130) sipW 2344101..2344673 (-) 573 WP_269195347.1 signal peptidase I SipW -
  O0R49_RS12135 (O0R49_12135) tapA 2344726..2345253 (-) 528 WP_269195348.1 amyloid fiber anchoring/assembly protein TapA -
  O0R49_RS12140 (O0R49_12140) - 2345553..2345831 (+) 279 WP_223251017.1 DUF3889 domain-containing protein -
  O0R49_RS12145 (O0R49_12145) - 2345865..2346062 (-) 198 WP_095409000.1 YqzE family protein -
  O0R49_RS12150 (O0R49_12150) - 2346122..2346505 (-) 384 WP_269195349.1 competence type IV pilus minor pilin ComGG -
  O0R49_RS12155 (O0R49_12155) comGF 2346502..2347029 (-) 528 WP_186437312.1 competence type IV pilus minor pilin ComGF -
  O0R49_RS12160 (O0R49_12160) comGE 2346941..2347255 (-) 315 WP_144484624.1 competence type IV pilus minor pilin ComGE -
  O0R49_RS12165 (O0R49_12165) comGD 2347239..2347685 (-) 447 WP_197198255.1 competence type IV pilus minor pilin ComGD -
  O0R49_RS12170 (O0R49_12170) comGC 2347678..2347971 (-) 294 WP_024422977.1 competence type IV pilus major pilin ComGC Machinery gene
  O0R49_RS12175 (O0R49_12175) comGB 2347988..2349028 (-) 1041 WP_269195350.1 competence type IV pilus assembly protein ComGB -
  O0R49_RS12180 (O0R49_12180) comGA 2349009..2350079 (-) 1071 WP_269195351.1 competence type IV pilus ATPase ComGA Machinery gene
  O0R49_RS12185 (O0R49_12185) - 2350257..2351132 (-) 876 WP_095409007.1 STAS domain-containing protein -
  O0R49_RS12195 (O0R49_12195) - 2351356..2351736 (-) 381 WP_007501232.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  O0R49_RS12200 (O0R49_12200) - 2351949..2352191 (+) 243 WP_003217440.1 DUF2626 domain-containing protein -
  O0R49_RS12205 (O0R49_12205) - 2352239..2352877 (-) 639 WP_095409008.1 MBL fold metallo-hydrolase -
  O0R49_RS12210 (O0R49_12210) - 2353035..2353208 (+) 174 WP_003217333.1 DUF2759 domain-containing protein -
  O0R49_RS12215 (O0R49_12215) - 2353432..2353746 (-) 315 WP_048238883.1 MTH1187 family thiamine-binding protein -
  O0R49_RS12220 (O0R49_12220) - 2353823..2355076 (-) 1254 WP_095409009.1 MFS transporter -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40436.92 Da        Isoelectric Point: 9.5040

>NTDB_id=764717 O0R49_RS12180 WP_269195351.1 2349009..2350079(-) (comGA) [Bacillus safensis strain PLA 1006]
MYGIEYLRQELLEEACRMRASDVHIVPKEKEASVSFRVDSDLIQQRTIDKKSGERLIAHFKFLSSMDIGEKRRPQNGSLA
VMLRKGQVFIRMSTLPTVNDESLVIRILPQDHVPKIKHLSLFPKASTRLLSFLKHSHGLILFTGPTNSGKTTTLYSLIQF
AKKNFNRNIITLEDPVETRNEEVLQVQVNEKAGITYAAGLRAILRHDPDMIVLGEIRDAETARTAIRAALTGHLVLSTLH
AKNAKGALYRMLEFGVTMNELEQTIVAIAAQRLIELICPFCGETCQLYCKLNRPFRRTNVFELLYGKDLGECIKEAKGEY
AHSSYETLQRLIRKGVALGYLSKSTYHRWVYEEASL

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=764717 O0R49_RS12180 WP_269195351.1 2349009..2350079(-) (comGA) [Bacillus safensis strain PLA 1006]
TTGTATGGGATTGAATATTTAAGGCAAGAGCTGCTAGAAGAAGCATGCCGCATGAGGGCTTCTGATGTGCATATTGTGCC
AAAAGAAAAGGAAGCTTCGGTGTCTTTCCGTGTTGACTCAGATTTAATCCAGCAGCGAACGATTGATAAAAAAAGCGGAG
AGAGGCTTATTGCACATTTTAAATTTTTATCTTCTATGGATATCGGGGAAAAAAGGAGACCGCAAAATGGCTCACTAGCT
GTGATGCTAAGAAAAGGACAAGTGTTTATTCGGATGTCTACCTTGCCGACAGTGAATGATGAGAGCTTAGTGATTAGAAT
TTTACCGCAGGATCATGTTCCGAAAATAAAACATCTATCATTATTTCCGAAAGCCTCAACTAGATTATTATCATTTTTGA
AGCATTCGCATGGACTCATTTTATTTACTGGGCCAACAAATTCAGGGAAAACAACAACACTATATTCATTAATCCAGTTT
GCAAAAAAGAATTTTAATCGAAATATTATTACCCTTGAAGACCCTGTCGAAACGAGGAATGAAGAAGTGTTACAGGTTCA
AGTGAATGAAAAAGCCGGCATCACGTATGCCGCTGGATTACGTGCTATTTTAAGACATGATCCAGATATGATTGTGCTGG
GAGAAATAAGAGATGCGGAAACGGCTAGAACGGCGATTAGGGCAGCACTGACAGGCCATTTAGTATTGAGTACGCTTCAT
GCAAAAAATGCAAAAGGAGCCCTTTACCGCATGCTTGAATTTGGTGTGACGATGAATGAACTCGAACAAACCATTGTTGC
GATTGCTGCCCAGCGATTAATTGAGCTTATATGCCCTTTTTGCGGGGAAACATGTCAGCTTTACTGTAAATTAAATCGTC
CTTTCAGACGAACAAATGTATTTGAATTGCTTTATGGAAAGGATCTTGGTGAGTGTATCAAAGAGGCTAAAGGAGAATAC
GCTCATTCGTCATACGAAACACTACAAAGATTAATTCGGAAAGGAGTGGCACTCGGTTATTTATCAAAAAGCACATATCA
TCGCTGGGTTTATGAAGAAGCGAGCCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

64.972

99.438

0.646