Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   OZ429_RS13115 Genome accession   NZ_CP114134
Coordinates   2782867..2783997 (-) Length   376 a.a.
NCBI ID   WP_269122265.1    Uniprot ID   -
Organism   Xanthomonas fragariae strain YLX21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2777867..2788997
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZ429_RS13085 (OZ429_13085) - 2778341..2778943 (-) 603 WP_269122253.1 glycine zipper 2TM domain-containing protein -
  OZ429_RS13090 (OZ429_13090) - 2779096..2779530 (-) 435 WP_208590101.1 OsmC family protein -
  OZ429_RS13095 (OZ429_13095) - 2779757..2780704 (-) 948 WP_269122257.1 aspartate carbamoyltransferase catalytic subunit -
  OZ429_RS13100 (OZ429_13100) ruvX 2780718..2781185 (-) 468 WP_269122259.1 Holliday junction resolvase RuvX -
  OZ429_RS13105 (OZ429_13105) - 2781178..2781744 (-) 567 WP_269122261.1 YqgE/AlgH family protein -
  OZ429_RS13110 (OZ429_13110) - 2782039..2782596 (+) 558 WP_269122263.1 DNA-3-methyladenine glycosylase I -
  OZ429_RS13115 (OZ429_13115) pilU 2782867..2783997 (-) 1131 WP_269122265.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  OZ429_RS13120 (OZ429_13120) pilT 2784119..2785156 (-) 1038 WP_269122267.1 type IV pilus twitching motility protein PilT Machinery gene
  OZ429_RS13125 (OZ429_13125) - 2785511..2786203 (+) 693 WP_269122269.1 YggS family pyridoxal phosphate-dependent enzyme -
  OZ429_RS13130 (OZ429_13130) proC 2786261..2787115 (+) 855 WP_269122271.1 pyrroline-5-carboxylate reductase -
  OZ429_RS13135 (OZ429_13135) - 2787680..2788105 (+) 426 WP_269126964.1 HU family DNA-binding protein -
  OZ429_RS13140 (OZ429_13140) - 2788212..2788853 (+) 642 WP_269126966.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41959.18 Da        Isoelectric Point: 6.5894

>NTDB_id=764501 OZ429_RS13115 WP_269122265.1 2782867..2783997(-) (pilU) [Xanthomonas fragariae strain YLX21]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTTLQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLAMTKRGIIVFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIVNFFPDERRNQLLMDLSLNLKGVIAQQLIPTPDGRSRRVAMEILLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVQ

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=764501 OZ429_RS13115 WP_269122265.1 2782867..2783997(-) (pilU) [Xanthomonas fragariae strain YLX21]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTTATCACTTCGGGAAT
GCCGCCGGCGATCAAGGTTAATGGGAAGATCAGTCCGATCACCCAGACCCCGTTGACCGCGCAGCAGAGCCGCGACCTTG
TGTTGAATGTGATGACCACATTGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAATTTCGCCATTGGCGTGTCCGGG
GTGGGGCGTTTTCGTGTGAGCTGTTTTTATCAGCGTAACCAGGTCGGCATGGTGCTGCGGCGGATTGAAACGCGCATTCC
CACCGTCGACGAATTGAGTCTGCCGCCGGTGATCAAGACGCTGGCAATGACCAAGCGCGGCATCATCGTTTTTGTCGGTG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCAGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGTTGCATCATCACCCAGCGTGAAGTCGGAATCGACACCGATAG
CTGGGAAAATGCGCTGAAAAATACCCTGCGCCAGGCACCGGATGTGATCATGATCGGTGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCGATTGCCTTCGCCGAAACCGGTCATCTGGTGCTGTGCACGCTGCATGCCAATAACGCCAACCAGGCGATG
GACCGCATCGTCAACTTCTTCCCGGACGAGCGCCGCAACCAGCTGCTGATGGATCTGTCGCTGAATCTCAAGGGCGTGAT
CGCGCAGCAGTTGATTCCGACCCCGGATGGACGCAGCCGTCGCGTGGCGATGGAAATCCTGCTGGGCACGCCGTTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACAAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTCTACCAGGCCGGTGAAATCAGCTACGAAGACGCGCTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAGGGTGGTGATGCCAAGACGCTGTCGCAGGGCCTGGACGGCGTGGAAATTGCCG
AGGTTCAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(83-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.933

94.681

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367
  pilT Acinetobacter nosocomialis M2

39.826

91.489

0.364
  pilT Acinetobacter baumannii D1279779

39.826

91.489

0.364
  pilT Acinetobacter baumannii strain A118

39.826

91.489

0.364