Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   O0R52_RS13195 Genome accession   NZ_CP114066
Coordinates   2616623..2617048 (-) Length   141 a.a.
NCBI ID   WP_256766241.1    Uniprot ID   -
Organism   Bacillus halotolerans strain B13     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2611623..2622048
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O0R52_RS13165 (O0R52_13165) - 2611863..2611997 (-) 135 WP_010335019.1 hypothetical protein -
  O0R52_RS13170 (O0R52_13170) - 2612075..2612806 (-) 732 WP_044154481.1 SGNH/GDSL hydrolase family protein -
  O0R52_RS13175 (O0R52_13175) - 2613053..2613805 (-) 753 WP_059335450.1 N-acetylmuramoyl-L-alanine amidase -
  O0R52_RS13180 (O0R52_13180) - 2614000..2614626 (+) 627 WP_024122122.1 TVP38/TMEM64 family protein -
  O0R52_RS13185 (O0R52_13185) gnd 2614647..2615540 (-) 894 WP_044154479.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  O0R52_RS13190 (O0R52_13190) - 2615861..2616589 (+) 729 WP_044154478.1 hypothetical protein -
  O0R52_RS13195 (O0R52_13195) nucA/comI 2616623..2617048 (-) 426 WP_256766241.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  O0R52_RS13200 (O0R52_13200) sigK 2617225..2617953 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  O0R52_RS13205 (O0R52_13205) - 2618074..2618247 (-) 174 Protein_2560 recombinase family protein -
  O0R52_RS13210 (O0R52_13210) - 2618205..2618383 (-) 179 Protein_2561 hypothetical protein -
  O0R52_RS13215 (O0R52_13215) - 2618985..2619695 (-) 711 WP_084995744.1 DUF421 domain-containing protein -
  O0R52_RS13220 (O0R52_13220) - 2619897..2620751 (+) 855 WP_084995746.1 ferritin family protein -
  O0R52_RS13225 (O0R52_13225) - 2621017..2621793 (-) 777 WP_222143204.1 sulfite exporter TauE/SafE family protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15526.71 Da        Isoelectric Point: 5.2215

>NTDB_id=764401 O0R52_RS13195 WP_256766241.1 2616623..2617048(-) (nucA/comI) [Bacillus halotolerans strain B13]
MKRWMAMLLLAAAVFLCLLGKDMTAPQQVKGAALYDKILYFPLSRYPETGDHIRDAIAEGHSDICTIDRDGADKRREESL
KGIPTKPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGAKVLFIVQ

Nucleotide


Download         Length: 426 bp        

>NTDB_id=764401 O0R52_RS13195 WP_256766241.1 2616623..2617048(-) (nucA/comI) [Bacillus halotolerans strain B13]
ATGAAAAGATGGATGGCAATGCTTCTTCTGGCGGCAGCGGTTTTTCTTTGTTTGTTGGGAAAGGATATGACGGCTCCGCA
ACAGGTCAAGGGGGCTGCTTTATATGACAAAATTTTATACTTTCCGTTGTCTCGTTATCCGGAAACCGGGGATCATATAA
GAGATGCGATTGCCGAAGGACATTCTGATATATGTACAATTGATCGTGATGGAGCGGACAAAAGGCGGGAGGAGTCATTA
AAAGGAATTCCGACGAAGCCTGGCTACGATCGGGATGAATGGCCGATGGCAGTTTGTGAAGAAGGAGGCGCCGGAGCCGA
TGTCCGATATGTAACGCCTTCTGATAATCGCGGCGCCGGTTCATGGGTAGGAAATCAAATGAGCAGTTATCCGGATGGCG
CCAAAGTGTTGTTTATTGTTCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(45-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

60.484

87.943

0.532