Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   QMY17_RS08865 Genome accession   NZ_CP125812
Coordinates   1683717..1684127 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain N3378-3At     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1678717..1689127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMY17_RS08840 - 1679822..1680712 (-) 891 WP_014480326.1 LysR family transcriptional regulator -
  QMY17_RS08845 fumC 1680879..1682267 (+) 1389 WP_014480325.1 class II fumarate hydratase -
  QMY17_RS08850 - 1682486..1682698 (+) 213 Protein_1725 recombinase family protein -
  QMY17_RS08855 - 1682695..1682793 (-) 99 WP_031600702.1 hypothetical protein -
  QMY17_RS08860 sigK 1682793..1683521 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  QMY17_RS08865 nucA/comI 1683717..1684127 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  QMY17_RS08870 yqeB 1684160..1684882 (-) 723 WP_014480321.1 hypothetical protein -
  QMY17_RS08875 gnd 1685133..1686026 (+) 894 WP_014480320.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  QMY17_RS08880 yqeD 1686045..1686671 (-) 627 WP_014480319.1 TVP38/TMEM64 family protein -
  QMY17_RS08885 cwlH 1686858..1687610 (+) 753 WP_014480318.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  QMY17_RS08890 yqeF 1687862..1688593 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  QMY17_RS08895 - 1688899..1689039 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=764126 QMY17_RS08865 WP_009967785.1 1683717..1684127(+) (nucA/comI) [Bacillus subtilis strain N3378-3At]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=764126 QMY17_RS08865 WP_009967785.1 1683717..1684127(+) (nucA/comI) [Bacillus subtilis strain N3378-3At]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529