Detailed information NTG0763930

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OZ175_RS25765
• Genome accession: NZ_CP113974
• Coordinates: 5527101..5528249 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain M6A146
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5522101..5533249

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OZ175_RS25740 (OZ175_25740)	-	5523252..5523548 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
OZ175_RS25745 (OZ175_25745)	-	5523545..5524138 (-)	594	WP_003084544.1	YggT family protein	-
OZ175_RS25750 (OZ175_25750)	proC	5524149..5524970 (-)	822	WP_268858368.1	pyrroline-5-carboxylate reductase	-
OZ175_RS25755 (OZ175_25755)	-	5524982..5525674 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
OZ175_RS25760 (OZ175_25760)	pilT	5525889..5526923 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
OZ175_RS25765 (OZ175_25765)	pilU	5527101..5528249 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
OZ175_RS25770 (OZ175_25770)	czcD	5528257..5529156 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
OZ175_RS25775 (OZ175_25775)	-	5529310..5529714 (+)	405	WP_003084560.1	NINE protein	-
OZ175_RS25780 (OZ175_25780)	-	5529957..5531330 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
OZ175_RS25785 (OZ175_25785)	-	5531327..5532511 (+)	1185	WP_023086800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=763930 OZ175_RS25765 WP_003100959.1 5527101..5528249(+) (pilU) [Pseudomonas aeruginosa strain M6A146]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=763930 OZ175_RS25765 WP_003100959.1 5527101..5528249(+) (pilU) [Pseudomonas aeruginosa strain M6A146]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382