Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OYW20_RS24570 Genome accession   NZ_CP113949
Coordinates   5436838..5437872 (-) Length   344 a.a.
NCBI ID   WP_268798451.1    Uniprot ID   -
Organism   Pseudomonas huanghezhanensis strain BSw22131     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5431838..5442872
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OYW20_RS24540 ruvX 5431887..5432327 (+) 441 WP_268798445.1 Holliday junction resolvase RuvX -
  OYW20_RS24545 pyrR 5432403..5432924 (+) 522 WP_268798446.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  OYW20_RS24550 - 5432951..5433955 (+) 1005 WP_268798447.1 aspartate carbamoyltransferase catalytic subunit -
  OYW20_RS24555 - 5433952..5435223 (+) 1272 WP_268798448.1 dihydroorotase -
  OYW20_RS24560 - 5435348..5435764 (-) 417 WP_268798449.1 TM2 domain-containing protein -
  OYW20_RS24565 - 5436015..5436749 (+) 735 WP_268798450.1 C40 family peptidase -
  OYW20_RS24570 pilT 5436838..5437872 (-) 1035 WP_268798451.1 type IV pilus twitching motility protein PilT Machinery gene
  OYW20_RS24575 - 5437927..5438613 (+) 687 WP_268798452.1 YggS family pyridoxal phosphate-dependent enzyme -
  OYW20_RS24580 proC 5438639..5439457 (+) 819 WP_268798453.1 pyrroline-5-carboxylate reductase -
  OYW20_RS24585 - 5439468..5440058 (+) 591 WP_268798454.1 YggT family protein -
  OYW20_RS24590 - 5440240..5441379 (+) 1140 WP_268798455.1 homoserine O-acetyltransferase -
  OYW20_RS24595 metW 5441387..5442007 (+) 621 WP_268798456.1 methionine biosynthesis protein MetW -
  OYW20_RS24600 - 5442043..5442495 (+) 453 WP_268798457.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37865.44 Da        Isoelectric Point: 6.7299

>NTDB_id=763773 OYW20_RS24570 WP_268798451.1 5436838..5437872(-) (pilT) [Pseudomonas huanghezhanensis strain BSw22131]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDQKQVQGLIYDIMNDKQRKDFEEDLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMVDYLNANKHHHILTIED
PIEFVHESKKSLINQREVHRDTHGFSEALRSALREDPDVILVGELRDLETIRLALTAAETGHLVFGTLHTTSAAKSIDRI
VDVFPAQEKSMIRSMLSESLHAIVSQALLKKVGGGRVAAHEIMIGTSAIRNLIREDKVAQMYSSIQTGGALGMQTLDMCL
KDLVSKGIITRESAREKAKTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=763773 OYW20_RS24570 WP_268798451.1 5436838..5437872(-) (pilT) [Pseudomonas huanghezhanensis strain BSw22131]
ATGGATATTACCGAGCTGCTTGCCTTCAGTGCCAAGCAAGGCGCGTCGGACTTACACCTCTCGGCTGGTCTGCCGCCGAT
GATTCGGGTCGACGGCGATGTGCGGCGAATCAACCTGCCGCCACTGGACCAGAAACAGGTTCAAGGGCTGATCTACGACA
TCATGAATGACAAGCAGCGCAAGGACTTCGAAGAAGATCTGGAAACGGACTTCTCGTTCGAAGTGCCAGGCGTCGCGCGC
TTTCGGGTCAACGCGTTCAACCAGAACCGGGGCGCAGGCGCTGTGTTTCGAACCATCCCGTCCAAGGTCCTGAGTATGGA
AGACCTGGGGATGGGAGAAGTGTTTCGCAAGATTACCGACGTGCCGCGCGGGCTGGTTCTGGTTACCGGGCCGACCGGCT
CGGGCAAGTCGACGACGCTGGCGGCGATGGTCGATTACCTCAATGCCAACAAACATCACCATATCCTCACGATTGAAGAC
CCTATCGAATTCGTTCACGAATCCAAGAAGAGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGTTTTTCCGA
GGCGTTGCGGTCGGCGCTGCGCGAAGACCCTGACGTGATTCTGGTGGGCGAGCTGCGAGACCTGGAAACGATCCGGCTGG
CGCTGACCGCTGCCGAGACTGGCCACCTTGTCTTCGGCACGCTGCACACCACGTCGGCGGCGAAAAGTATCGACCGCATC
GTCGATGTGTTCCCGGCGCAGGAGAAGTCGATGATTCGTTCGATGCTGTCTGAGTCGCTACACGCAATCGTGTCGCAGGC
GCTGCTGAAGAAGGTGGGCGGCGGGCGAGTGGCGGCTCACGAGATCATGATCGGCACGTCGGCGATCCGAAACCTGATCC
GCGAAGACAAGGTCGCGCAGATGTATTCGTCGATTCAGACTGGCGGGGCGCTTGGGATGCAGACGCTGGACATGTGCCTC
AAGGACCTGGTCAGCAAAGGCATCATCACGCGAGAAAGCGCCCGCGAGAAAGCAAAAACGCCCGACAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.244

100

0.892
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

79.651

100

0.797
  pilT Acinetobacter baumannii D1279779

79.36

100

0.794
  pilT Acinetobacter baumannii strain A118

79.36

100

0.794
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

73.373

98.256

0.721
  pilT Legionella pneumophila strain ERS1305867

73.373

98.256

0.721
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

68.249

97.965

0.669
  pilT Vibrio cholerae strain A1552

68.249

97.965

0.669
  pilT Neisseria meningitidis 8013

66.377

100

0.666
  pilT Neisseria gonorrhoeae MS11

66.087

100

0.663
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Pseudomonas stutzeri DSM 10701

41.791

97.384

0.407
  pilU Acinetobacter baylyi ADP1

39.595

100

0.398
  pilU Vibrio cholerae strain A1552

38.261

100

0.384