Detailed information NTG0763756

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OYW20_RS13340
• Genome accession: NZ_CP113949
• Coordinates: 2998055..2999230 (-)
• Length: 391 a.a.
• NCBI ID: WP_268796459.1
• Uniprot ID: -
• Organism: Pseudomonas huanghezhanensis strain BSw22131
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2993055..3004230

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OYW20_RS13320	-	2993379..2994530 (-)	1152	WP_268796455.1	phytanoyl-CoA dioxygenase family protein	-
OYW20_RS13325	-	2994527..2995387 (-)	861	WP_268796456.1	SDR family oxidoreductase	-
OYW20_RS13330	-	2995562..2996587 (+)	1026	WP_268796457.1	LacI family DNA-binding transcriptional regulator	-
OYW20_RS13335	iolG	2996662..2997660 (+)	999	WP_268796458.1	inositol 2-dehydrogenase	-
OYW20_RS13340	pilU	2998055..2999230 (-)	1176	WP_268796459.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
OYW20_RS13345	-	2999392..2999667 (+)	276	WP_237254458.1	peptidylprolyl isomerase	-
OYW20_RS13350	-	2999778..3000179 (-)	402	Protein_2667	PfkB family carbohydrate kinase	-
OYW20_RS13360	-	3001132..3002355 (+)	1224	WP_268796461.1	MFS transporter	-
OYW20_RS13365	-	3002488..3003429 (-)	942	WP_268796462.1	sugar kinase	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 43365.59 Da
• Isoelectric Point: 6.3928

>NTDB_id=763756 OYW20_RS13340 WP_268796459.1 2998055..2999230(-) (pilU) [Pseudomonas huanghezhanensis strain BSw22131]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLSTEALKPGDVAKIAESIMDSEQRHDFERDLEMNLAYSLGGIGR
FRINIFKQRNEVSVVARNIKLDIPKFEDLHLPPVLLDVVMEKRGLVLFVGATGSGKSTSLAALIDHRNRHASGHIITIED
PIEFIHKHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLQDLGNNLKAFVSQRLVKTMDGKRRAAVEVMLGTPTIRDLIHRNELTELKGIMDKSTNLGMQTFDNAL
YDLAVEGVITEEEALKNADSMNNVRLRLKLHSENAVTDITTPPPAPQPAAQVNVADWGLVDEKDEPGTPHF

Nucleotide

• Length: 1176 bp

>NTDB_id=763756 OYW20_RS13340 WP_268796459.1 2998055..2999230(-) (pilU) [Pseudomonas huanghezhanensis strain BSw22131]
ATGGATTTTCCGGCGTTGTTGAAGATTCTGGCCAGCCAGGACGGATCGGACCTTTATCTTTCCACCGGCGCGCCACCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCCCTGAGTACCGAGGCCTTGAAGCCCGGCGATGTCGCGAAAATCGCCGAATCCA
TCATGGACAGCGAACAGCGGCATGACTTCGAGCGTGATCTGGAAATGAACCTGGCCTATTCACTGGGCGGCATCGGGCGT
TTCCGGATCAACATTTTCAAGCAGCGTAACGAAGTGTCTGTCGTGGCGCGAAACATCAAACTCGACATTCCCAAATTCGA
AGACCTGCACCTGCCGCCTGTGTTGCTTGATGTGGTCATGGAAAAACGCGGTCTGGTGTTGTTCGTCGGCGCAACCGGCT
CTGGTAAATCGACTTCGCTCGCCGCGCTGATTGACCATCGCAATCGCCACGCCAGCGGGCACATCATCACCATCGAAGAC
CCGATCGAGTTCATTCACAAGCACAAGAAGTCGATCATCAACCAGCGCGAAGTCGGCGTTGATACGCGCAGTTTCCACGC
CGCCCTGAAAAACACCCTGCGTCAGGCGCCGGACGTGATCCTGATCGGTGAGATCCGCGACCGGGAAACCATGGAGCATG
CGCTGGCATTTGCGGACACAGGGCACCTGGCGATTTCGACGCTGCATGCCAACAACGCCAACCAGGCCCTGGACCGTATC
ATCAACTTCTTCCCGGAAGAACGCCGCCCGCAGTTGCTTCAGGACTTGGGTAACAACCTCAAGGCGTTCGTGTCGCAGCG
ACTGGTCAAGACCATGGACGGCAAGCGCCGCGCGGCGGTGGAGGTGATGCTCGGCACGCCGACCATTCGTGATCTGATCC
ATCGCAACGAATTGACCGAACTCAAAGGCATCATGGATAAGTCCACCAACCTGGGCATGCAGACGTTCGACAACGCCCTG
TACGACCTGGCCGTGGAAGGCGTGATTACAGAAGAGGAAGCGCTGAAGAACGCTGACTCGATGAACAACGTACGCCTGCG
CCTGAAACTGCACAGCGAAAACGCCGTCACTGACATCACCACACCGCCGCCAGCCCCCCAACCGGCCGCACAGGTGAATG
TCGCGGACTGGGGCCTGGTAGACGAAAAAGACGAACCGGGCACGCCGCATTTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.192	90.537	0.527
pilU	Acinetobacter baylyi ADP1	53.803	90.793	0.488
pilU	Vibrio cholerae strain A1552	51.714	89.514	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.773	86.701	0.371
pilT	Pseudomonas stutzeri DSM 10701	41.399	87.724	0.363
pilT	Pseudomonas aeruginosa PAK	41.716	86.445	0.361