Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   OZX61_RS01865 Genome accession   NZ_CP113932
Coordinates   406898..407695 (+) Length   265 a.a.
NCBI ID   WP_195771394.1    Uniprot ID   -
Organism   Acinetobacter sp. ESL0695     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 401898..412695
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZX61_RS01835 (OZX61_01835) ilvN 402466..402957 (+) 492 WP_195771400.1 acetolactate synthase small subunit -
  OZX61_RS01840 (OZX61_01840) ilvC 402992..404008 (+) 1017 WP_195771399.1 ketol-acid reductoisomerase -
  OZX61_RS01845 (OZX61_01845) - 404159..404710 (+) 552 WP_277095122.1 phosphatase PAP2 family protein -
  OZX61_RS01850 (OZX61_01850) iscX 404791..404991 (+) 201 WP_195771397.1 Fe-S cluster assembly protein IscX -
  OZX61_RS01855 (OZX61_01855) ndk 405097..405528 (+) 432 WP_195771396.1 nucleoside-diphosphate kinase -
  OZX61_RS01860 (OZX61_01860) rlmN 405648..406877 (+) 1230 WP_195771395.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  OZX61_RS01865 (OZX61_01865) pilF 406898..407695 (+) 798 WP_195771394.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  OZX61_RS01870 (OZX61_01870) - 407686..408471 (+) 786 WP_195771393.1 helix-turn-helix domain-containing protein -
  OZX61_RS01875 (OZX61_01875) ispG 408494..409612 (+) 1119 WP_277095123.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  OZX61_RS01880 (OZX61_01880) hisS 409609..410898 (+) 1290 WP_277095125.1 histidine--tRNA ligase -
  OZX61_RS01885 (OZX61_01885) - 410933..411640 (+) 708 WP_277095127.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29863.69 Da        Isoelectric Point: 8.9459

>NTDB_id=763613 OZX61_RS01865 WP_195771394.1 406898..407695(+) (pilF) [Acinetobacter sp. ESL0695]
MKKISSYAVVGLILSLTLQGCQTTQSNLKQDPKKSAEVRTQIAAEYIRSGDLDAAKRSLDEALQKDDRDATANMMMGVLL
QQEGSPSNLEKADRYFKRAISLNSTDAQIRNNYGRYLYQLGRYNEAEKQLQIAGSTLGYDQRAMALENLGQTYLRLGNTT
AAEQAFMSALRADQSSPVALVELAEIMYQRQQYNTASDFYEGYVRIVGSKNQSAQALWVGIRIARANGDNMSMQILVNQL
RALYPDSQEYQRYLKLQYSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=763613 OZX61_RS01865 WP_195771394.1 406898..407695(+) (pilF) [Acinetobacter sp. ESL0695]
GTGAAAAAAATATCATCATATGCAGTTGTGGGGCTAATTTTAAGTTTAACCTTACAGGGGTGCCAGACCACCCAAAGCAA
TCTGAAGCAAGATCCTAAAAAATCTGCTGAAGTAAGAACACAAATTGCAGCAGAATATATTCGTTCTGGCGATTTGGATG
CAGCAAAACGGTCTTTAGATGAGGCATTACAAAAAGATGACCGTGATGCAACTGCAAATATGATGATGGGTGTGTTATTA
CAACAAGAAGGTAGTCCAAGTAACCTTGAAAAAGCAGATCGTTACTTTAAAAGAGCAATTTCTTTAAATTCAACAGATGC
GCAAATTCGTAATAACTATGGTCGTTATCTTTACCAATTGGGTCGCTATAATGAGGCAGAGAAGCAATTACAAATTGCGG
GCTCTACATTAGGTTACGATCAAAGAGCCATGGCCTTAGAAAATCTTGGACAAACCTATTTGCGTTTGGGGAATACAACT
GCTGCTGAGCAGGCTTTCATGAGTGCACTAAGAGCTGATCAGAGTTCTCCTGTTGCTTTGGTAGAATTGGCAGAAATTAT
GTATCAGCGCCAACAATATAATACGGCTTCAGATTTTTATGAGGGCTATGTACGTATTGTTGGTAGCAAAAATCAAAGTG
CTCAAGCACTTTGGGTTGGAATTCGCATTGCGCGTGCGAATGGTGATAATATGAGTATGCAGATCTTGGTGAATCAGTTG
CGCGCATTATATCCTGATAGCCAAGAATATCAGCGTTATTTGAAATTACAGTACAGTACTGAGGCGGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(107-174)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

63.498

99.245

0.63
  pilF Acinetobacter baylyi ADP1

59.848

99.623

0.596