Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OUI59_RS06010 Genome accession   NZ_CP113789
Coordinates   1239139..1240119 (-) Length   326 a.a.
NCBI ID   WP_136086749.1    Uniprot ID   -
Organism   Klebsiella variicola subsp. tropica strain BSK_177V2     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1234139..1245119
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OUI59_RS05980 (OUI59_05980) endA 1234429..1235136 (+) 708 WP_100631832.1 deoxyribonuclease I -
  OUI59_RS05985 (OUI59_05985) rsmE 1235228..1235959 (+) 732 WP_061153332.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OUI59_RS05990 (OUI59_05990) gshB 1235980..1236930 (+) 951 WP_268657696.1 glutathione synthase -
  OUI59_RS05995 (OUI59_05995) - 1237105..1237668 (+) 564 WP_012540566.1 YqgE/AlgH family protein -
  OUI59_RS06000 (OUI59_06000) ruvX 1237668..1238084 (+) 417 WP_061156319.1 Holliday junction resolvase RuvX -
  OUI59_RS06005 (OUI59_06005) - 1238137..1238790 (-) 654 WP_074387594.1 LuxR family transcriptional regulator -
  OUI59_RS06010 (OUI59_06010) pilT 1239139..1240119 (-) 981 WP_136086749.1 type IV pilus twitching motility protein PilT Machinery gene
  OUI59_RS06015 (OUI59_06015) - 1240136..1240837 (+) 702 WP_061153329.1 YggS family pyridoxal phosphate-dependent enzyme -
  OUI59_RS06020 (OUI59_06020) - 1240858..1241424 (+) 567 WP_004205182.1 YggT family protein -
  OUI59_RS06025 (OUI59_06025) yggU 1241421..1241711 (+) 291 WP_002916614.1 DUF167 family protein YggU -
  OUI59_RS06030 (OUI59_06030) - 1241724..1242317 (+) 594 WP_074387592.1 XTP/dITP diphosphatase -
  OUI59_RS06035 (OUI59_06035) hemW 1242310..1243446 (+) 1137 WP_016161832.1 radical SAM family heme chaperone HemW -
  OUI59_RS06040 (OUI59_06040) - 1243487..1244206 (-) 720 WP_012540560.1 DUF2884 domain-containing protein -
  OUI59_RS06045 (OUI59_06045) - 1244257..1244583 (-) 327 WP_012540559.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35407.96 Da        Isoelectric Point: 6.9549

>NTDB_id=763090 OUI59_RS06010 WP_136086749.1 1239139..1240119(-) (pilT) [Klebsiella variicola subsp. tropica strain BSK_177V2]
MKLEEIVALSVKHNVSDLHLCNSAAPRWRRQGKLEPAPFPAPDILDLLHCRLDAAQLQHWQEQGQIDFALTLACGSRLRA
SAFAHTRGISLVLRLLPEQCPRLETLGAPPALSELLAEESGLLLVTGATGSGKSTTLAAMVGHLNQRLDGHILTLEDPVE
FIHHSERCLIQQREIGRHCPSFAAALRVALRQDPDVILLGELRDSETIRLALTAAETGHLVMATLHTRGAAPAVERLIDV
FPAEEKDQVRSQLAGSLCAVLAQKLLPARQGGRVALYELLVNTPAVANLIREGKVHQLPGVMQTGMQAGMLTFTQSFQQR
VAAGEL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=763090 OUI59_RS06010 WP_136086749.1 1239139..1240119(-) (pilT) [Klebsiella variicola subsp. tropica strain BSK_177V2]
ATGAAGCTGGAAGAAATCGTAGCCCTTAGTGTAAAGCATAATGTCTCCGATCTACACCTGTGCAATTCCGCCGCACCGCG
CTGGCGGCGGCAGGGCAAGCTGGAGCCCGCCCCGTTTCCCGCGCCGGATATCCTGGATTTACTCCACTGCAGGCTTGATG
CCGCACAGTTACAGCACTGGCAGGAACAGGGCCAGATTGACTTTGCGCTGACCCTGGCCTGCGGTTCGCGGCTGCGCGCC
AGCGCTTTCGCCCATACGCGTGGAATATCGCTGGTGCTACGCCTTCTGCCTGAACAGTGCCCGCGTCTGGAGACGCTGGG
CGCCCCGCCTGCGCTGAGCGAGCTGCTGGCGGAAGAGAGTGGCCTGCTGCTGGTCACCGGAGCGACGGGCAGCGGCAAGT
CGACCACCCTGGCGGCGATGGTGGGGCATCTCAACCAGCGTCTTGATGGCCATATTCTGACCCTGGAGGACCCGGTGGAG
TTTATTCATCACAGCGAGCGCTGCCTGATCCAGCAGCGGGAGATTGGCCGTCACTGTCCTTCATTCGCCGCGGCGCTGCG
CGTGGCGCTGCGCCAGGATCCGGATGTGATCCTGCTCGGCGAGCTACGCGACAGCGAAACCATCCGTCTGGCGTTAACGG
CTGCGGAGACCGGGCATCTGGTGATGGCGACGTTACACACCCGCGGTGCGGCGCCGGCAGTGGAGAGATTGATCGATGTC
TTTCCAGCAGAGGAGAAAGATCAGGTTCGTAGTCAGCTGGCCGGAAGCCTGTGCGCGGTGCTGGCGCAAAAATTGTTACC
TGCACGTCAGGGCGGGAGAGTTGCGCTATACGAGCTTTTGGTCAATACCCCCGCCGTGGCGAATTTGATCCGTGAAGGAA
AAGTGCACCAGTTGCCCGGCGTGATGCAGACCGGGATGCAGGCCGGAATGCTGACATTTACGCAGAGTTTTCAGCAACGT
GTCGCCGCAGGCGAGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.376

100

0.515
  pilT Vibrio cholerae strain A1552

51.376

100

0.515
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

47.401

100

0.475
  pilT Legionella pneumophila strain ERS1305867

47.531

99.387

0.472
  pilT Legionella pneumophila strain Lp02

47.531

99.387

0.472
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.164

97.239

0.429
  pilU Vibrio cholerae strain A1552

40.426

100

0.408
  pilU Pseudomonas stutzeri DSM 10701

40.303

100

0.408
  pilU Acinetobacter baylyi ADP1

38.941

98.466

0.383